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Molecular and Cellular Biology, January 1999, p. 909-915, Vol. 19, No. 1
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Selected Elements of Herpes Simplex Virus Accessory Factor HCF Are Highly Conserved in Caenorhabditis elegans

Yi Liu, Michael O. Hengartner, and Winship Herr*

Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724

Received 23 June 1998/Returned for modification 23 July 1998/Accepted 7 October 1998

HCF is a mammalian nuclear protein that undergoes proteolytic processing and is required for cell proliferation. During productive herpes simplex virus (HSV) infection, the viral transactivator VP16 associates with HCF to initiate HSV gene transcription. Here, we show that the worm Caenorhabditis elegans possesses a functional homolog of mammalian HCF that can associate with and activate the viral protein VP16. The pattern of sequence conservation, however, is uneven. Sequences required for mammalian HCF processing are not present in C. elegans HCF. Furthermore, not all elements of mammalian HCF that are required for promoting cell proliferation are conserved. Nevertheless, unexpectedly, C. elegans HCF can promote mammalian cell proliferation because a region of HCF that is conserved can promote mammalian cell proliferation better than its human counterpart. These results suggest that HCF possesses a highly conserved role in metazoan cell proliferation which is targeted by VP16 to regulate HSV infection. The precise mechanisms, however, by which HCF functions in mammals and worms appear to differ.

* Corresponding author. Mailing address: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724. Phone: (516) 367-8401. Fax: (516) 367-8454. E-mail: herr{at}cshl.org.

Molecular and Cellular Biology, January 1999, p. 909-915, Vol. 19, No. 1
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lee, S., Herr, W. (2001). Stabilization but Not the Transcriptional Activity of Herpes Simplex Virus VP16-Induced Complexes Is Evolutionarily Conserved among HCF Family Members. J. Virol. 75: 12402-12411 [Abstract] [Full Text]  
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  • Lu, R., Misra, V. (2000). Zhangfei: a second cellular protein interacts with herpes simplex virus accessory factor HCF in a manner similar to Luman and VP16. Nucleic Acids Res 28: 2446-2454 [Abstract] [Full Text]  
  • Mahajan, S. S., Wilson, A. C. (2000). Mutations in Host Cell Factor 1 Separate Its Role in Cell Proliferation from Recruitment of VP16 and LZIP. Mol. Cell. Biol. 20: 919-928 [Abstract] [Full Text]  
  • HERR, W. (1998). The Herpes Simplex Virus VP16-induced Complex: Mechanisms of Combinatorial Transcriptional Regulation. Cold Spring Harb Symp Quant Biol 63: 599-608 [Abstract]  
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