This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Johnston, S. D.
Right arrow Articles by Berman, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Johnston, S. D.
Right arrow Articles by Berman, J.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, January 1999, p. 923-933, Vol. 19, No. 1
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Gbp1p, a Protein with RNA Recognition Motifs, Binds Single-Stranded Telomeric DNA and Changes Its Binding Specificity upon Dimerization

Stephen D. Johnston, Jodi E. Lew, and Judith Berman*

Department of Plant Biology, University of Minnesota, St. Paul, Minnesota 55108

Received 18 June 1998/Returned for modification 2 August 1998/Accepted 20 October 1998

Gbp1p is a putative telomere-binding protein from Chlamydomonas reinhardtii that contains two RNA recognition motifs (RRMs) which are commonly found in heterogeneous nuclear ribonucleoproteins (hnRNPs). Previously we demonstrated that Gbp1p binds single-stranded DNA (ssDNA) containing the Chlamydomonas telomeric sequence but not the RNA containing the cognate sequence. Here we show that at lower protein concentrations Gbp1 can also bind an RNA containing the cognate sequence. We found that mutation of the two RRM motifs of Gbp1p to match the highly conserved region of hnRNP RRMs did not alter the affinity of Gbp1p for either RNA or DNA. The ability of Gbp1p to associate with either of these two nucleic acids is governed by the dimerization state of the protein. Monomeric Gbp1p associates with either ssDNA or RNA, showing a small binding preference for RNA. Dimeric Gbp1p has a strong preference for binding ssDNA and shows little affinity for RNA. To the best of our knowledge, this is the first example of a protein that qualitatively shifts its nucleic acid binding preference upon dimerization. The biological implications of a telomere-binding protein that is regulated by dimerization are discussed.

* Corresponding author. Mailing address: Department of Plant Biology, University of Minnesota, 220 Biological Sciences Center, 1445 Gortner Ave., St. Paul, MN 55108. Phone: (612) 625-1971. Fax: (612) 625-1738. E-mail: berma003{at}tc.umn.edu.

Molecular and Cellular Biology, January 1999, p. 923-933, Vol. 19, No. 1
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Benjamin, L. R., Chung, H.-J., Sanford, S., Kouzine, F., Liu, J., Levens, D. (2008). Hierarchical mechanisms build the DNA-binding specificity of FUSE binding protein. Proc. Natl. Acad. Sci. USA 105: 18296-18301 [Abstract] [Full Text]  
  • Messaoudi, L., Yang, Y.-G., Kinomura, A., Stavreva, D. A., Yan, G., Bortolin-Cavaille, M.-L., Arakawa, H., Buerstedde, J.-M., Hainaut, P., Cavaille, J., Takata, M., Van Dyck, E. (2007). Subcellular distribution of human RDM1 protein isoforms and their nucleolar accumulation in response to heat shock and proteotoxic stress. Nucleic Acids Res 35: 6571-6587 [Abstract] [Full Text]  
  • Suswam, E. A., Li, Y. Y., Mahtani, H., King, P. H. (2005). Novel DNA-binding properties of the RNA-binding protein TIAR. Nucleic Acids Res 33: 4507-4518 [Abstract] [Full Text]  
  • Myojin, R., Kuwahara, S., Yasaki, T., Matsunaga, T., Sakurai, T., Kimura, M., Uesugi, S., Kurihara, Y. (2004). Expression and Functional Significance of Mouse Paraspeckle Protein 1 on Spermatogenesis. Biol. Reprod. 71: 926-932 [Abstract] [Full Text]  
  • Shay, J. W., Zou, Y., Hiyama, E., Wright, W. E. (2001). Telomerase and cancer. Hum Mol Genet 10: 677-685 [Abstract] [Full Text]  
  • Ford, L. P., Suh, J. M., Wright, W. E., Shay, J. W. (2000). Heterogeneous Nuclear Ribonucleoproteins C1 and C2 Associate with the RNA Component of Human Telomerase. Mol. Cell. Biol. 20: 9084-9091 [Abstract] [Full Text]  
  • Dallaire, F., Dupuis, S., Fiset, S., Chabot, B. (2000). Heterogeneous Nuclear Ribonucleoprotein A1 and UP1 Protect Mammalian Telomeric Repeats and Modulate Telomere Replication in Vitro. J. Biol. Chem. 275: 14509-14516 [Abstract] [Full Text]  
  • Zhou, J., Hidaka, K., Futcher, B. (2000). The Est1 Subunit of Yeast Telomerase Binds the Tlc1 Telomerase RNA. Mol. Cell. Biol. 20: 1947-1955 [Abstract] [Full Text]  
  • Ding, J., Hayashi, M. K., Zhang, Y., Manche, L., Krainer, A. R., Xu, R.-M. (1999). Crystal structure of the two-RRM domain of hnRNP A1 (UP1) complexed with single-stranded telomeric DNA. Genes Dev. 13: 1102-1115 [Abstract] [Full Text]  
  • Chennathukuzhi, V. M., Kurihara, Y., Bray, J. D., Hecht, N. B. (2001). Trax (Translin-associated Factor X), a Primarily Cytoplasmic Protein, Inhibits the Binding of TB-RBP (Translin) to RNA. J. Biol. Chem. 276: 13256-13263 [Abstract] [Full Text]  
  • Cano, M. I. N., Blake, J. J., Blackburn, E. H., Agabian, N. (2002). A Trypanosoma brucei Protein Complex That Binds G-overhangs and Co-purifies with Telomerase Activity. J. Biol. Chem. 277: 896-906 [Abstract] [Full Text]  
  • Buratti, E., Baralle, F. E. (2001). Characterization and Functional Implications of the RNA Binding Properties of Nuclear Factor TDP-43, a Novel Splicing Regulator of CFTR Exon 9. J. Biol. Chem. 276: 36337-36343 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»