This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lai, A.
Right arrow Articles by Branton, P. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lai, A.
Right arrow Articles by Branton, P. E.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 1999, p. 6632-6641, Vol. 19, No. 10
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

RBP1 Recruits Both Histone Deacetylase-Dependent and -Independent Repression Activities to Retinoblastoma Family Proteins

Albert Lai,1 Joseph M. Lee,1 Wen-Ming Yang,2 James A. DeCaprio,3 William G. Kaelin Jr.,3,4 Edward Seto,2 and Philip E. Branton1,5,*

Departments of Biochemistry1 and Oncology,5 McGill University, Montreal, Quebec, Canada H3G 1Y6; H. Lee Moffitt Cancer Center and Research Institute, Molecular Oncology Program, University of South Florida, Tampa, Florida 336122; and Dana-Farber Cancer Institute and Harvard Medical School3 and Howard Hughes Medical Institute,4 Boston, Massachusetts 02115

Received 3 May 1999/Returned for modification 1 June 1999/Accepted 18 June 1999

Retinoblastoma (RB) tumor suppressor family proteins block cell proliferation in part by repressing certain E2F-specific promoters. Both histone deacetylase (HDAC)-dependent and -independent repression activities are associated with the RB "pocket." The mechanism by which these two repression functions occupy the pocket is unknown. A known RB-binding protein, RBP1, was previously found by our group to be an active corepressor which, if overexpressed, represses E2F-mediated transcription via its association with the pocket. We show here that RBP1 contains two repression domains, one of which binds all three known HDACs and represses them in an HDAC-dependent manner while the other domain functions independently of the HDACs. Thus, RB family members repress transcription by recruiting RBP1 to the pocket. RBP1, in turn, serves as a bridging molecule to recruit HDACs and, in addition, provides a second HDAC-independent repression function.

* Corresponding author. Mailing address: Departments of Biochemistry and Oncology, McGill University, McIntyre Medical Building, 3655 Drummond St., Montreal, Quebec, Canada H3G 1Y6. Phone: (514) 398-7268. Fax: (514) 398-7384. E-mail: branton{at}med.mcgill.ca.

Molecular and Cellular Biology, October 1999, p. 6632-6641, Vol. 19, No. 10
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ishimaru, N., Arakaki, R., Yoshida, S., Yamada, A., Noji, S., Hayashi, Y. (2008). Expression of the retinoblastoma protein RbAp48 in exocrine glands leads to Sjogren's syndrome-like autoimmune exocrinopathy. JEM 205: 2915-2927 [Abstract] [Full Text]  
  • Wu, M.-Y., Eldin, K. W., Beaudet, A. L. (2008). Identification of Chromatin Remodeling Genes Arid4a and Arid4b as Leukemia Suppressor Genes. JNCI J Natl Cancer Inst 100: 1247-1259 [Abstract] [Full Text]  
  • Seifried, L. A., Talluri, S., Cecchini, M., Julian, L. M., Mymryk, J. S., Dick, F. A. (2008). pRB-E2F1 Complexes Are Resistant to Adenovirus E1A-Mediated Disruption. J. Virol. 82: 4511-4520 [Abstract] [Full Text]  
  • Hurst, D. R., Xie, Y., Vaidya, K. S., Mehta, A., Moore, B. P., Accavitti-Loper, M. A., Samant, R. S., Saxena, R., Silveira, A. C., Welch, D. R. (2008). Alterations of BRMS1-ARID4A Interaction Modify Gene Expression but Still Suppress Metastasis in Human Breast Cancer Cells. J. Biol. Chem. 283: 7438-7444 [Abstract] [Full Text]  
  • Kong, L., Yu, X.-P., Bai, X.-H., Zhang, W.-F., Zhang, Y., Zhao, W.-M., Jia, J.-H., Tang, W., Zhou, Y.-B., Liu, C.-j. (2007). RbAp48 Is a Critical Mediator Controlling the Transforming Activity of Human Papillomavirus Type 16 in Cervical Cancer. J. Biol. Chem. 282: 26381-26391 [Abstract] [Full Text]  
  • Escaffit, F., Vaute, O., Chevillard-Briet, M., Segui, B., Takami, Y., Nakayama, T., Trouche, D. (2007). Cleavage and Cytoplasmic Relocalization of Histone Deacetylase 3 Are Important for Apoptosis Progression. Mol. Cell. Biol. 27: 554-567 [Abstract] [Full Text]  
  • Wu, M.-Y., Tsai, T.-F., Beaudet, A. L. (2006). Deficiency of Rbbp1/Arid4a and Rbbp1l1/Arid4b alters epigenetic modifications and suppresses an imprinting defect in the PWS/AS domain.. Genes Dev. 20: 2859-2870 [Abstract] [Full Text]  
  • Monroe, D. G., Secreto, F. J., Hawse, J. R., Subramaniam, M., Khosla, S., Spelsberg, T. C. (2006). Estrogen Receptor Isoform-specific Regulation of the Retinoblastoma-binding Protein 1 (RBBP1) Gene: ROLES OF AF1 AND ENHANCER ELEMENTS. J. Biol. Chem. 281: 28596-28604 [Abstract] [Full Text]  
  • Harbour, J. W. (2006). Eye Cancer: Unique Insights into Oncogenesis The Cogan Lecture. IOVS 47: 1737-1745 [Full Text]  
  • Ishimaru, N., Arakaki, R., Omotehara, F., Yamada, K., Mishima, K., Saito, I., Hayashi, Y. (2006). Novel Role for RbAp48 in Tissue-Specific, Estrogen Deficiency-Dependent Apoptosis in the Exocrine Glands. Mol. Cell. Biol. 26: 2924-2935 [Abstract] [Full Text]  
  • Binda, O., Roy, J.-S., Branton, P. E. (2006). RBP1 Family Proteins Exhibit SUMOylation-Dependent Transcriptional Repression and Induce Cell Growth Inhibition Reminiscent of Senescence.. Mol. Cell. Biol. 26: 1917-1931 [Abstract] [Full Text]  
  • Singh, M., Krajewski, M., Mikolajka, A., Holak, T. A. (2005). Molecular Determinants for the Complex Formation between the Retinoblastoma Protein and LXCXE Sequences. J. Biol. Chem. 280: 37868-37876 [Abstract] [Full Text]  
  • Meloni, A. R., Lai, C.-H., Yao, T.-P., Nevins, J. R. (2005). A Mechanism of COOH-Terminal Binding Protein-Mediated Repression. Mol Cancer Res 3: 575-583 [Abstract] [Full Text]  
  • Gray, S. G., Iglesias, A. H., Lizcano, F., Villanueva, R., Camelo, S., Jingu, H., Teh, B. T., Koibuchi, N., Chin, W. W., Kokkotou, E., Dangond, F. (2005). Functional Characterization of JMJD2A, a Histone Deacetylase- and Retinoblastoma-binding Protein. J. Biol. Chem. 280: 28507-28518 [Abstract] [Full Text]  
  • Wright, E., Bain, M., Teague, L., Murphy, J., Sinclair, J. (2005). Ets-2 repressor factor recruits histone deacetylase to silence human cytomegalovirus immediate-early gene expression in non-permissive cells. J. Gen. Virol. 86: 535-544 [Abstract] [Full Text]  
  • Patsialou, A., Wilsker, D., Moran, E. (2005). DNA-binding properties of ARID family proteins. Nucleic Acids Res 33: 66-80 [Abstract] [Full Text]  
  • Ding, Z., Gillespie, L. L., Mercer, F. C., Paterno, G. D. (2004). The SANT Domain of Human MI-ER1 Interacts with Sp1 to Interfere with GC Box Recognition and Repress Transcription from Its Own Promoter. J. Biol. Chem. 279: 28009-28016 [Abstract] [Full Text]  
  • Pennaneach, V., Barbier, V., Regazzoni, K., Fotedar, R., Fotedar, A. (2004). Rb Inhibits E2F-1-induced Cell Death in a LXCXE-dependent Manner by Active Repression. J. Biol. Chem. 279: 23376-23383 [Abstract] [Full Text]  
  • Meehan, W. J., Samant, R. S., Hopper, J. E., Carrozza, M. J., Shevde, L. A., Workman, J. L., Eckert, K. A., Verderame, M. F., Welch, D. R. (2004). Breast Cancer Metastasis Suppressor 1 (BRMS1) Forms Complexes with Retinoblastoma-binding Protein 1 (RBP1) and the mSin3 Histone Deacetylase Complex and Represses Transcription. J. Biol. Chem. 279: 1562-1569 [Abstract] [Full Text]  
  • Siddiqui, H., Solomon, D. A., Gunawardena, R. W., Wang, Y., Knudsen, E. S. (2003). Histone Deacetylation of RB-Responsive Promoters: Requisite for Specific Gene Repression but Dispensable for Cell Cycle Inhibition. Mol. Cell. Biol. 23: 7719-7731 [Abstract] [Full Text]  
  • Childs, K. S., Goodbourn, S. (2003). Identification of novel co-repressor molecules for Interferon Regulatory Factor-2. Nucleic Acids Res 31: 3016-3026 [Abstract] [Full Text]  
  • Balasenthil, S., Vadlamudi, R. K. (2003). Functional Interactions between the Estrogen Receptor Coactivator PELP1/MNAR and Retinoblastoma Protein. J. Biol. Chem. 278: 22119-22127 [Abstract] [Full Text]  
  • Fleischer, T. C., Yun, U. J., Ayer, D. E. (2003). Identification and Characterization of Three New Components of the mSin3A Corepressor Complex. Mol. Cell. Biol. 23: 3456-3467 [Abstract] [Full Text]  
  • Zhang, Y., Woodford, N., Xia, X., Hamburger, A. W. (2003). Repression of E2F1-mediated transcription by the ErbB3 binding protein Ebp1 involves histone deacetylases. Nucleic Acids Res 31: 2168-2177 [Abstract] [Full Text]  
  • Wen, Y.-D., Cress, W. D., Roy, A. L., Seto, E. (2003). Histone Deacetylase 3 Binds to and Regulates the Multifunctional Transcription Factor TFII-I. J. Biol. Chem. 278: 1841-1847 [Abstract] [Full Text]  
  • Yochum, G. S., Ayer, D. E. (2002). Role for the Mortality Factors MORF4, MRGX, and MRG15 in Transcriptional Repression via Associations with Pf1, mSin3A, and Transducin-Like Enhancer of Split. Mol. Cell. Biol. 22: 7868-7876 [Abstract] [Full Text]  
  • Zhang, W., Kone, B. C. (2002). NF-kappa B inhibits transcription of the H+-K+-ATPase alpha 2-subunit gene: role of histone deacetylases. Am. J. Physiol. Renal Physiol. 283: F904-F911 [Abstract] [Full Text]  
  • Anderson, L. A., Perkins, N. D. (2002). The Large Subunit of Replication Factor C Interacts with the Histone Deacetylase, HDAC1. J. Biol. Chem. 277: 29550-29554 [Abstract] [Full Text]  
  • Yu, Z., Zhang, W., Kone, B. C. (2002). Histone Deacetylases Augment Cytokine Induction of the iNOS Gene. J. Am. Soc. Nephrol. 13: 2009-2017 [Abstract] [Full Text]  
  • Cook, J. L., Walker, T. A., Worthen, G. S., Radke, J. R. (2002). Role of the E1A Rb-binding domain in repression of the NF-kappa B-dependent defense against tumor necrosis factor-alpha. Proc. Natl. Acad. Sci. USA 99: 9966-9971 [Abstract] [Full Text]  
  • Sullivan, C. S., Pipas, J. M. (2002). T Antigens of Simian Virus 40: Molecular Chaperones for Viral Replication and Tumorigenesis. Microbiol. Mol. Biol. Rev. 66: 179-202 [Abstract] [Full Text]  
  • Galasinski, S. C., Resing, K. A., Goodrich, J. A., Ahn, N. G. (2002). Phosphatase Inhibition Leads to Histone Deacetylases 1 and 2 Phosphorylation and Disruption of Corepressor Interactions. J. Biol. Chem. 277: 19618-19626 [Abstract] [Full Text]  
  • Rayman, J. B., Takahashi, Y., Indjeian, V. B., Dannenberg, J.-H., Catchpole, S., Watson, R. J., te Riele, H., Dynlacht, B. D. (2002). E2F mediates cell cycle-dependent transcriptional repression in vivo by recruitment of an HDAC1/mSin3B corepressor complex. Genes Dev. 16: 933-947 [Abstract] [Full Text]  
  • Wilsker, D., Patsialou, A., Dallas, P. B., Moran, E. (2002). ARID Proteins: A Diverse Family of DNA Binding Proteins Implicated in the Control of Cell Growth, Differentiation, and Development. Cell Growth Differ. 13: 95-106 [Abstract] [Full Text]  
  • Kuzmichev, A., Zhang, Y., Erdjument-Bromage, H., Tempst, P., Reinberg, D. (2002). Role of the Sin3-Histone Deacetylase Complex in Growth Regulation by the Candidate Tumor Suppressor p33ING1. Mol. Cell. Biol. 22: 835-848 [Abstract] [Full Text]  
  • Nicolas, E., Ait-Si-Ali, S., Trouche, D. (2001). The histone deacetylase HDAC3 targets RbAp48 to the retinoblastoma protein. Nucleic Acids Res 29: 3131-3136 [Abstract] [Full Text]  
  • Kennedy, B. K., Liu, O. W., Dick, F. A., Dyson, N., Harlow, E., Vidal, M. (2001). Histone deacetylase-dependent transcriptional repression by pRB in yeast occurs independently of interaction through the LXCXE binding cleft. Proc. Natl. Acad. Sci. USA 10.1073/pnas.151240898v1 [Abstract] [Full Text]  
  • Lai, A., Kennedy, B. K., Barbie, D. A., Bertos, N. R., Yang, X. J., Theberge, M.-C., Tsai, S.-C., Seto, E., Zhang, Y., Kuzmichev, A., Lane, W. S., Reinberg, D., Harlow, E., Branton, P. E. (2001). RBP1 Recruits the mSIN3-Histone Deacetylase Complex to the Pocket of Retinoblastoma Tumor Suppressor Family Proteins Found in Limited Discrete Regions of the Nucleus at Growth Arrest. Mol. Cell. Biol. 21: 2918-2932 [Abstract] [Full Text]  
  • Ross, J. F., Näär, A., Cam, H., Gregory, R., Dynlacht, B. D. (2001). Active repression and E2F inhibition by pRB are biochemically distinguishable. Genes Dev. 15: 392-397 [Abstract] [Full Text]  
  • Hirsch, H. A., Gu, L., Henry, R. W. (2000). The Retinoblastoma Tumor Suppressor Protein Targets Distinct General Transcription Factors To Regulate RNA Polymerase III Gene Expression. Mol. Cell. Biol. 20: 9182-9191 [Abstract] [Full Text]  
  • Knudsen, K. E., Booth, D., Naderi, S., Sever-Chroneos, Z., Fribourg, A. F., Hunton, I. C., Feramisco, J. R., Wang, J. Y. J., Knudsen, E. S. (2000). RB-Dependent S-Phase Response to DNA Damage. Mol. Cell. Biol. 20: 7751-7763 [Abstract] [Full Text]  
  • BURKE, L. J., BANIAHMAD, A. (2000). Co-repressors 2000. FASEB J. 14: 1876-1888 [Abstract] [Full Text]  
  • Harbour, J. W., Dean, D. C. (2000). The Rb/E2F pathway: expanding roles and emerging paradigms. Genes Dev. 14: 2393-2409 [Full Text]  
  • Dallas, P. B., Pacchione, S., Wilsker, D., Bowrin, V., Kobayashi, R., Moran, E. (2000). The Human SWI-SNF Complex Protein p270 Is an ARID Family Member with Non-Sequence-Specific DNA Binding Activity. Mol. Cell. Biol. 20: 3137-3146 [Abstract] [Full Text]  
  • Lee, S.-K., Kim, J.-H., Lee, Y. C., Cheong, J., Lee, J. W. (2000). Silencing Mediator of Retinoic Acid and Thyroid Hormone Receptors, as a Novel Transcriptional Corepressor Molecule of Activating Protein-1, Nuclear Factor-kappa B, and Serum Response Factor. J. Biol. Chem. 275: 12470-12474 [Abstract] [Full Text]  
  • Nicolas, E., Morales, V., Magnaghi-Jaulin, L., Harel-Bellan, A., Richard-Foy, H., Trouche, D. (2000). RbAp48 Belongs to the Histone Deacetylase Complex That Associates with the Retinoblastoma Protein. J. Biol. Chem. 275: 9797-9804 [Abstract] [Full Text]  
  • Koipally, J., Georgopoulos, K. (2000). Ikaros Interactions with CtBP Reveal a Repression Mechanism That Is Independent of Histone Deacetylase Activity. J. Biol. Chem. 275: 19594-19602 [Abstract] [Full Text]  
  • Jang, M. K., Goo, Y. H., Sohn, Y. C., Kim, Y. S., Lee, S.-K., Kang, H., Cheong, J., Lee, J. W. (2001). Ca2+/Calmodulin-dependent Protein Kinase IV Stimulates Nuclear Factor-kappa B Transactivation via Phosphorylation of the p65 Subunit. J. Biol. Chem. 276: 20005-20010 [Abstract] [Full Text]  
  • Kennedy, B. K., Liu, O. W., Dick, F. A., Dyson, N., Harlow, E., Vidal, M. (2001). Histone deacetylase-dependent transcriptional repression by pRB in yeast occurs independently of interaction through the LXCXE binding cleft. Proc. Natl. Acad. Sci. USA 98: 8720-8725 [Abstract] [Full Text]  
  • Jiang, Z., Zacksenhaus, E. (2002). Activation of retinoblastoma protein in mammary gland leads to ductal growth suppression, precocious differentiation, and adenocarcinoma. JCB 156: 185-198 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»