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Molecular and Cellular Biology, October 1999, p. 6972-6979, Vol. 19, No. 10
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Rsp5 Ubiquitin-Protein Ligase Mediates DNA Damage-Induced Degradation of the Large Subunit of RNA Polymerase II in Saccharomyces cerevisiae

Sylvie L. Beaudenon,1 Maria R. Huacani,2 Guangli Wang,1 Donald P. McDonnell,2 and Jon M. Huibregtse1,*

Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08855,1 and Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 277102

Received 26 April 1999/Returned for modification 10 June 1999/Accepted 1 July 1999

Rsp5 is an E3 ubiquitin-protein ligase of Saccharomyces cerevisiae that belongs to the hect domain family of E3 proteins. We have previously shown that Rsp5 binds and ubiquitinates the largest subunit of RNA polymerase II, Rpb1, in vitro. We show here that Rpb1 ubiquitination and degradation are induced in vivo by UV irradiation and by the UV-mimetic compound 4-nitroquinoline-1-oxide (4-NQO) and that a functional RSP5 gene product is required for this effect. The 26S proteasome is also required; a mutation of SEN3/RPN2 (sen3-1), which encodes an essential regulatory subunit of the 26S proteasome, partially blocks 4-NQO-induced degradation of Rpb1. These results suggest that Rsp5-mediated ubiquitination and degradation of Rpb1 are components of the response to DNA damage. A human WW domain-containing hect (WW-hect) E3 protein closely related to Rsp5, Rpf1/hNedd4, also binds and ubiquitinates both yeast and human Rpb1 in vitro, suggesting that Rpf1 and/or another WW-hect E3 protein mediates UV-induced degradation of the large subunit of polymerase II in human cells.

* Corresponding author. Mailing address: Department of Molecular Biology and Biochemistry, Rutgers University, Picataway, NJ 08855. Phone: (732) 445-0938. Fax: (732) 445-4213. E-mail: huibregt{at}waksman.rutgers.edu.

Molecular and Cellular Biology, October 1999, p. 6972-6979, Vol. 19, No. 10
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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