MCB
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Delva, L.
Right arrow Articles by Chomienne, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Delva, L.
Right arrow Articles by Chomienne, C.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, October 1999, p. 7158-7167, Vol. 19, No. 10
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Physical and Functional Interactions between Cellular Retinoic Acid Binding Protein II and the Retinoic Acid-Dependent Nuclear Complex

Laurent Delva,1 Jean-Noël Bastie,1 Cécile Rochette-Egly,2 Radhia Kraïba,1 Nicole Balitrand,1 Gilles Despouy,1 Pierre Chambon,2 and Christine Chomienne1,*

Laboratoire de Biologie Cellulaire Hématopoïétique, EP-107 CNRS, Université D. Diderot-Paris VII, Institut d'Hématologie, Hôpital Saint-Louis, 75010 Paris,1 and Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP/Collège de France, 67404 Illkirch Cedex, CU de Strasbourg,2 France

Received 8 March 1999/Returned for modification 4 April 1999/Accepted 4 July 1999

Two sorts of proteins bind to, and mediate the developmental and homeostatic effects of, retinoic acid (RA): the RAR and RXR nuclear receptors, which act as ligand-dependent transcriptional regulators, and the cellular RA binding proteins (CRABPI and CRABPII). CRABPs are generally known to be implicated in the synthesis, degradation, and control of steady-state levels of RA, yet previous and recent data have indicated that they could play a role in the control of gene expression. Here we show for the first time that, both in vitro and in vivo, CRABPII is associated with RARalpha and RXRalpha in a ligand-independent manner in mammalian cells (HL-60, NB-4, and MCF-7). In the nucleus, this protein complex binds the RXR-RAR-specific response element of an RA target gene (RARE-DR5). Moreover, in the presence of retinoids that bind both the nuclear receptors and CRABPII, enhancement of transactivation by RXRalpha -RARalpha heterodimers is observed in the presence of CRABPII. Thus, CRABPII appears to be a novel transcriptional regulator involved in RA signaling.


* Corresponding author. Mailing address: Laboratoire de Biologie Cellulaire Hématopoïétique (LBCH), EP-107 CNRS, Université D. Diderot-Paris VII, Institut d'Hématologie, Hôpital Saint-Louis, 75010 Paris, France. Phone: 33 0 1 42 40 97 45. Fax: 33 0 1 42 00 01 60. E-mail: lbch{at}chu-stlouis.fr.


Molecular and Cellular Biology, October 1999, p. 7158-7167, Vol. 19, No. 10
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. J. Virol. Eukaryot. Cell
Microbiol. Mol. Biol. Rev. Clin. Vaccine Immunol. All ASM Journals

Copyright © 1999 by the American Society for Microbiology. All rights reserved.