The Mre11-Rad50-Xrs2 Protein Complex Facilitates Homologous Recombination-Based Double-Strand Break Repair in Saccharomyces cerevisiae

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Bressan, D. A.
	Articles by Petrini, J. H. J.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Bressan, D. A.
	Articles by Petrini, J. H. J.

Previous Article | Next Article

Molecular and Cellular Biology, November 1999, p. 7681-7687, Vol. 19, No. 11
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Mre11-Rad50-Xrs2 Protein Complex Facilitates Homologous Recombination-Based Double-Strand Break Repair in Saccharomyces cerevisiae

Debra A. Bressan, Bonnie K. Baxter, and John H. J. Petrini^*

Laboratory of Genetics, University of Wisconsin Medical School, Madison, Wisconsin 53706

Received 16 June 1999/Returned for modification 30 July 1999/Accepted 19 August 1999

Saccharomyces cerevisiae mre11 $Delta$ mutants are profoundly deficient in double-strand break (DSB) repair, indicating that theMre11-Rad50-Xrs2 protein complex plays a central role in the cellularresponse to DNA DSBs. In this study, we examined the role of thecomplex in homologous recombination, the primary mode of DSB repairin yeast. We measured survival in synchronous cultures followingirradiation and scored sister chromatid and interhomologue recombinationgenetically. mre11 $Delta$ strains were profoundly sensitive to ionizingradiation (IR) throughout the cell cycle. Mutant strains exhibiteddecreased frequencies of IR-induced sister chromatid and interhomologuerecombination, indicating a general deficiency in homologous recombination-basedDSB repair. Since a nuclease-deficient mre11 mutant was not impairedin these assays, it appears that the role of the S. cerevisiaeMre11-Rad50-Xrs2 protein complex in facilitating homologous recombinationis independent of its nucleaseactivities.

^* Corresponding author. Mailing address: Laboratory of Genetics, University of Wisconsin Medical School, 445 Henry Mall, Madison,WI 53706. Phone: (608) 265-6043. Fax: (608) 262-2976. E-mail:jpetrini{at}facstaff.wisc.edu.

Manuscript 3538 from the University of Wisconsin $---$ Madison Laboratory ofGenetics.

Molecular and Cellular Biology, November 1999, p. 7681-7687, Vol. 19, No. 11
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

Coic, E., Feldman, T., Landman, A. S., Haber, J. E. (2008). Mechanisms of Rad52-Independent Spontaneous and UV-Induced Mitotic Recombination in Saccharomyces cerevisiae. Genetics 179: 199-211 [Abstract] [Full Text]
Wu, D., Topper, L. M., Wilson, T. E. (2008). Recruitment and Dissociation of Nonhomologous End Joining Proteins at a DNA Double-Strand Break in Saccharomyces cerevisiae. Genetics 178: 1237-1249 [Abstract] [Full Text]
Cullen, J. K., Hussey, S. P., Walker, C., Prudden, J., Wee, B.-Y., Dave, A., Findlay, J. S., Savory, A. P., Humphrey, T. C. (2007). Break-Induced Loss of Heterozygosity in Fission Yeast: Dual Roles for Homologous Recombination in Promoting Translocations and Preventing De Novo Telomere Addition. Mol. Cell. Biol. 27: 7745-7757 [Abstract] [Full Text]
Game, J. C., Williamson, M. S., Spicakova, T., Brown, J. M. (2006). The RAD6/BRE1 Histone Modification Pathway in Saccharomyces Confers Radiation Resistance Through a RAD51-Dependent Process That Is Independent of RAD18. Genetics 173: 1951-1968 [Abstract] [Full Text]
Cohen, P. E., Pollack, S. E., Pollard, J. W. (2006). Genetic Analysis of Chromosome Pairing, Recombination, and Cell Cycle Control during First Meiotic Prophase in Mammals. Endocr. Rev. 27: 398-426 [Abstract] [Full Text]
Mercier, G., Berthault, N., Touleimat, N., Kepes, F., Fourel, G., Gilson, E., Dutreix, M. (2005). A haploid-specific transcriptional response to irradiation in Saccharomyces cerevisiae. Nucleic Acids Res 33: 6635-6643 [Abstract] [Full Text]
Clerici, M., Mantiero, D., Lucchini, G., Longhese, M. P. (2005). The Saccharomyces cerevisiae Sae2 Protein Promotes Resection and Bridging of Double Strand Break Ends. J. Biol. Chem. 280: 38631-38638 [Abstract] [Full Text]
Hope, J. C., Maftahi, M., Freyer, G. A. (2005). A Postsynaptic Role for Rhp55/57 That Is Responsible for Cell Death in {Delta}rqh1 Mutants Following Replication Arrest in Schizosaccharomyces pombe. Genetics 170: 519-531 [Abstract] [Full Text]
Jiang, W.-Q., Zhong, Z.-H., Henson, J. D., Neumann, A. A., Chang, A. C.-M., Reddel, R. R. (2005). Suppression of Alternative Lengthening of Telomeres by Sp100-Mediated Sequestration of the MRE11/RAD50/NBS1 Complex. Mol. Cell. Biol. 25: 2708-2721 [Abstract] [Full Text]
Barber, L. J., Ward, T. A., Hartley, J. A., McHugh, P. J. (2005). DNA Interstrand Cross-Link Repair in the Saccharomyces cerevisiae Cell Cycle: Overlapping Roles for PSO2 (SNM1) with MutS Factors and EXO1 during S Phase. Mol. Cell. Biol. 25: 2297-2309 [Abstract] [Full Text]
Chen, L., Trujillo, K. M., Van Komen, S., Roh, D. H., Krejci, L., Lewis, L. K., Resnick, M. A., Sung, P., Tomkinson, A. E. (2005). Effect of Amino Acid Substitutions in the Rad50 ATP Binding Domain on DNA Double Strand Break Repair in Yeast. J. Biol. Chem. 280: 2620-2627 [Abstract] [Full Text]
Nag, D. K., Suri, M., Stenson, E. K. (2004). Both CAG repeats and inverted DNA repeats stimulate spontaneous unequal sister-chromatid exchange in Saccharomyces cerevisiae. Nucleic Acids Res 32: 5677-5684 [Abstract] [Full Text]
Demuth, I., Frappart, P.-O., Hildebrand, G., Melchers, A., Lobitz, S., Stockl, L., Varon, R., Herceg, Z., Sperling, K., Wang, Z.-Q., Digweed, M. (2004). An inducible null mutant murine model of Nijmegen breakage syndrome proves the essential function of NBS1 in chromosomal stability and cell viability. Hum Mol Genet 13: 2385-2397 [Abstract] [Full Text]
Baroni, E., Viscardi, V., Cartagena-Lirola, H., Lucchini, G., Longhese, M. P. (2004). The Functions of Budding Yeast Sae2 in the DNA Damage Response Require Mec1- and Tel1-Dependent Phosphorylation. Mol. Cell. Biol. 24: 4151-4165 [Abstract] [Full Text]
Cheng, W.-H., von Kobbe, C., Opresko, P. L., Arthur, L. M., Komatsu, K., Seidman, M. M., Carney, J. P., Bohr, V. A. (2004). Linkage between Werner Syndrome Protein and the Mre11 Complex via Nbs1. J. Biol. Chem. 279: 21169-21176 [Abstract] [Full Text]
Lewis, L. K., Storici, F., Van Komen, S., Calero, S., Sung, P., Resnick, M. A. (2004). Role of the Nuclease Activity of Saccharomyces cerevisiae Mre11 in Repair of DNA Double-Strand Breaks in Mitotic Cells. Genetics 166: 1701-1713 [Abstract] [Full Text]
Davis, A. P., Symington, L. S. (2004). RAD51-Dependent Break-Induced Replication in Yeast. Mol. Cell. Biol. 24: 2344-2351 [Abstract] [Full Text]
Grandin, N., Charbonneau, M. (2003). Mitotic Cyclins Regulate Telomeric Recombination in Telomerase-Deficient Yeast Cells. Mol. Cell. Biol. 23: 9162-9177 [Abstract] [Full Text]
Lee, J.-H., Ghirlando, R., Bhaskara, V., Hoffmeyer, M. R., Gu, J., Paull, T. T. (2003). Regulation of Mre11/Rad50 by Nbs1: EFFECTS ON NUCLEOTIDE-DEPENDENT DNA BINDING AND ASSOCIATION WITH ATAXIA-TELANGIECTASIA-LIKE DISORDER MUTANT COMPLEXES. J. Biol. Chem. 278: 45171-45181 [Abstract] [Full Text]
Allen, C., Halbrook, J., Nickoloff, J. A. (2003). Interactive Competition Between Homologous Recombination and Non-Homologous End Joining. Mol Cancer Res 1: 913-920 [Abstract] [Full Text]
Chahwan, C., Nakamura, T. M., Sivakumar, S., Russell, P., Rhind, N. (2003). The Fission Yeast Rad32 (Mre11)-Rad50-Nbs1 Complex Is Required for the S-Phase DNA Damage Checkpoint. Mol. Cell. Biol. 23: 6564-6573 [Abstract] [Full Text]
Tomita, K., Matsuura, A., Caspari, T., Carr, A. M., Akamatsu, Y., Iwasaki, H., Mizuno, K.-i., Ohta, K., Uritani, M., Ushimaru, T., Yoshinaga, K., Ueno, M. (2003). Competition between the Rad50 Complex and the Ku Heterodimer Reveals a Role for Exo1 in Processing Double-Strand Breaks but Not Telomeres. Mol. Cell. Biol. 23: 5186-5197 [Abstract] [Full Text]
Yoshida, J., Umezu, K., Maki, H. (2003). Positive and Negative Roles of Homologous Recombination in the Maintenance of Genome Stability in Saccharomyces cerevisiae. Genetics 164: 31-46 [Abstract] [Full Text]
Mirzoeva, O. K., Petrini, J. H.J. (2003). DNA Replication-Dependent Nuclear Dynamics of the Mre11 Complex. Mol Cancer Res 1: 207-218 [Abstract] [Full Text]
Chang, M., Bellaoui, M., Boone, C., Brown, G. W. (2002). A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage. Proc. Natl. Acad. Sci. USA 99: 16934-16939 [Abstract] [Full Text]
Symington, L. S. (2002). Role of RAD52 Epistasis Group Genes in Homologous Recombination and Double-Strand Break Repair. Microbiol. Mol. Biol. Rev. 66: 630-670 [Abstract] [Full Text]
Tseng, H.-M., Tomkinson, A. E. (2002). A Physical and Functional Interaction between Yeast Pol4 and Dnl4-Lif1 Links DNA Synthesis and Ligation in Nonhomologous End Joining. J. Biol. Chem. 277: 45630-45637 [Abstract] [Full Text]
Cummings, W. J., Merino, S. T., Young, K. G., Li, L., Johnson, C. W., Sierra, E. A., Zolan, M. E. (2002). The Coprinus cinereus adherin Rad9 functions in Mre11-dependent DNA repair, meiotic sister-chromatid cohesion, and meiotic homolog pairing. Proc. Natl. Acad. Sci. USA 99: 14958-14963 [Abstract] [Full Text]
Morgan, E. A., Shah, N., Symington, L. S. (2002). The Requirement for ATP Hydrolysis by Saccharomyces cerevisiae Rad51 Is Bypassed by Mating-Type Heterozygosity or RAD54 in High Copy. Mol. Cell. Biol. 22: 6336-6343 [Abstract] [Full Text]
de Jager, M., Kanaar, R. (2002). Genome instability and Rad50S: subtle yet severe. Genes Dev. 16: 2173-2178 [Full Text]
Bender, C. F., Sikes, M. L., Sullivan, R., Huye, L. E., Le Beau, M. M., Roth, D. B., Mirzoeva, O. K., Oltz, E. M., Petrini, J. H. J. (2002). Cancer predisposition and hematopoietic failure in Rad50S/S mice. Genes Dev. 16: 2237-2251 [Abstract] [Full Text]
Manthey, G. M., Bailis, A. M. (2002). Multiple Pathways Promote Short-Sequence Recombination in Saccharomyces cerevisiae. Mol. Cell. Biol. 22: 5347-5356 [Abstract] [Full Text]
Kolodner, R. D., Putnam, C. D., Myung, K. (2002). Maintenance of Genome Stability in Saccharomyces cerevisiae. Science 297: 552-557 [Abstract] [Full Text]
Robinson, N. P., McCulloch, R., Conway, C., Browitt, A., Barry, J. D. (2002). Inactivation of Mre11 Does Not Affect VSG Gene Duplication Mediated by Homologous Recombination in Trypanosoma brucei. J. Biol. Chem. 277: 26185-26193 [Abstract] [Full Text]
Cohen, Y., Dardalhon, M., Averbeck, D. (2002). Homologous recombination is essential for RAD51 up-regulation in Saccharomyces cerevisiae following DNA crosslinking damage. Nucleic Acids Res 30: 1224-1232 [Abstract] [Full Text]
Lewis, L. K., Karthikeyan, G., Westmoreland, J. W., Resnick, M. A. (2002). Differential Suppression of DNA Repair Deficiencies of Yeast rad50, mre11 and xrs2 Mutants by EXO1 and TLC1 (the RNA Component of Telomerase). Genetics 160: 49-62 [Abstract] [Full Text]
Bucholc, M., Park, Y., Lustig, A. J. (2001). Intrachromatid Excision of Telomeric DNA as a Mechanism for Telomere Size Control in Saccharomyces cerevisiae. Mol. Cell. Biol. 21: 6559-6573 [Abstract] [Full Text]
Maser, R. S., Mirzoeva, O. K., Wells, J., Olivares, H., Williams, B. R., Zinkel, R. A., Farnham, P. J., Petrini, J. H. J. (2001). Mre11 Complex and DNA Replication: Linkage to E2F and Sites of DNA Synthesis. Mol. Cell. Biol. 21: 6006-6016 [Abstract] [Full Text]
Sonoda, E., Takata, M., Yamashita, Y. M., Morrison, C., Takeda, S. (2001). Homologous DNA recombination in vertebrate cells. Proc. Natl. Acad. Sci. USA 98: 8388-8394 [Abstract] [Full Text]
Fasullo, M., Giallanza, P., Dong, Z., Cera, C., Bennett, T. (2001). Saccharomyces cerevisiae rad51 Mutants Are Defective in DNA Damage-Associated Sister Chromatid Exchanges but Exhibit Increased Rates of Homology-Directed Translocations. Genetics 158: 959-972 [Abstract] [Full Text]
Signon, L., Malkova, A., Naylor, M. L., Klein, H., Haber, J. E. (2001). Genetic Requirements for RAD51- and RAD54-Independent Break-Induced Replication Repair of a Chromosomal Double-Strand Break. Mol. Cell. Biol. 21: 2048-2056 [Abstract] [Full Text]
de Jager, M., Dronkert, M. L. G., Modesti, M., Beerens, C. E. M. T., Kanaar, R., van Gent, D. C. (2001). DNA-binding and strand-annealing activities of human Mre11: implications for its roles in DNA double-strand break repair pathways. Nucleic Acids Res 29: 1317-1325 [Abstract] [Full Text]
Chin, G. M., Villeneuve, A. M. (2001). C. elegans mre-11 is required for meiotic recombination and DNA repair but is dispensable for the meiotic G2 DNA damage checkpoint. Genes Dev. 15: 522-534 [Abstract] [Full Text]
Clikeman, J. A., Khalsa, G. J., Barton, S. L., Nickoloff, J. A. (2001). Homologous Recombinational Repair of Double-Strand Breaks in Yeast Is Enhanced by MAT Heterozygosity Through yKU-Dependent and -Independent Mechanisms. Genetics 157: 579-589 [Abstract] [Full Text]
Mirzoeva, O. K., Petrini, J. H. J. (2001). DNA Damage-Dependent Nuclear Dynamics of the Mre11 Complex. Mol. Cell. Biol. 21: 281-288 [Abstract] [Full Text]
Symington, L. S., Kang, L. E., Moreau, S. (2000). Alteration of gene conversion tract length and associated crossing over during plasmid gap repair in nuclease-deficient strains of Saccharomyces cerevisiae. Nucleic Acids Res 28: 4649-4656 [Abstract] [Full Text]
Hopfner, K.-P., Karcher, A., Shin, D., Fairley, C., Tainer, J. A., Carney, J. P. (2000). Mre11 and Rad50 from Pyrococcus furiosus: Cloning and Biochemical Characterization Reveal an Evolutionarily Conserved Multiprotein Machine. J. Bacteriol. 182: 6036-6041 [Abstract] [Full Text]
Merino, S. T., Cummings, W. J., Acharya, S. N., Zolan, M. E. (2000). Replication-dependent early meiotic requirement for Spo11 and Rad50. Proc. Natl. Acad. Sci. USA 10.1073/pnas.190346097v1 [Abstract] [Full Text]
Girard, P.-M., Foray, N., Stumm, M., Waugh, A., Riballo, E., Maser, R. S., Phillips, W. P., Petrini, J., Arlett, C. F., Jeggo, P. A. (2000). Radiosensitivity in Nijmegen Breakage Syndrome Cells Is Attributable to a Repair Defect and not Cell Cycle Checkpoint Defects. Cancer Res. 60: 4881-4888 [Abstract] [Full Text]
Lee, K.-J., Huang, J., Takeda, Y., Dynan, W. S. (2000). DNA Ligase IV and XRCC4 Form a Stable Mixed Tetramer That Functions Synergistically with Other Repair Factors in a Cell-free End-joining System. J. Biol. Chem. 275: 34787-34796 [Abstract] [Full Text]
Paull, T. T., Gellert, M. (2000). A mechanistic basis for Mre11-directed DNA joining at microhomologies. Proc. Natl. Acad. Sci. USA 97: 6409-6414 [Abstract] [Full Text]
Paull, T. T., Cortez, D., Bowers, B., Elledge, S. J., Gellert, M. (2001). From the Cover: Direct DNA binding by Brca1. Proc. Natl. Acad. Sci. USA 98: 6086-6091 [Abstract] [Full Text]
Merino, S. T., Cummings, W. J., Acharya, S. N., Zolan, M. E. (2000). Replication-dependent early meiotic requirement for Spo11 and Rad50. Proc. Natl. Acad. Sci. USA 97: 10477-10482 [Abstract] [Full Text]

J. Bacteriol.	J. Virol.	Eukaryot. Cell

Microbiol. Mol. Biol. Rev.	Clin. Vaccine Immunol.	All ASM Journals