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Molecular and Cellular Biology, February 1999, p. 1470-1478, Vol. 19, No. 2
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The SWI/SNF Complex Creates Loop Domains in DNA and Polynucleosome Arrays and Can Disrupt DNA-Histone Contacts within These Domains

David P. Bazett-Jones,1,* Jacques Côté,2 Carolyn C. Landel,3 Craig L. Peterson,3 and Jerry L. Workman4

Department of Cell Biology and Anatomy, University of Calgary, Calgary, Alberta, Canada T2N 4N11; Laval University Cancer Research Centre, Hôtel-Dieu de Québec, Québec City, Québec, Canada G1R 2J62; Howard Hughes Medical Institute and Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802-45004; and Department of Biochemistry and Molecular Biology, Program in Molecular Medicine, University of Massachusetts, Worcester, Massachusetts 016053

Received 7 July 1998/Returned for modification 21 August 1998/Accepted 21 September 1998

To understand the mechanisms by which the chromatin-remodeling SWI/SNF complex interacts with DNA and alters nucleosome organization, we have imaged the SWI/SNF complex with both naked DNA and nucleosomal arrays by using energy-filtered microscopy. By making ATP-independent contacts with DNA at multiple sites on its surface, SWI/SNF creates loops, bringing otherwise-distant sites into close proximity. In the presence of ATP, SWI/SNF action leads to the disruption of nucleosomes within domains that appear to be topologically constrained by the complex. The data indicate that the action of one SWI/SNF complex on an array of nucleosomes can lead to the formation of a region where multiple nucleosomes are disrupted. Importantly, nucleosome disruption by SWI/SNF results in a loss of DNA content from the nucleosomes. This indicates a mechanism by which SWI/SNF unwraps part of the nucleosomal DNA.

* Corresponding author. Mailing address: Department of Cell Biology and Anatomy, University of Calgary, Calgary, AB, Canada T2N 4N1. Phone: (403) 220-3025. Fax: (403) 270-0737. E-mail: bazett{at}acs.ucalgary.ca.

Molecular and Cellular Biology, February 1999, p. 1470-1478, Vol. 19, No. 2
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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