This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Han, S. J.
Right arrow Articles by Kim, Y.-J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Han, S. J.
Right arrow Articles by Kim, Y.-J.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, February 1999, p. 979-988, Vol. 19, No. 2
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Activator-Specific Requirement of Yeast Mediator Proteins for RNA Polymerase II Transcriptional Activation

Sang Jun Han,1 Young Chul Lee,1,dagger Byung Soo Gim,1 Gi-Hyuck Ryu,1 Soon Jung Park,1,Dagger William S. Lane,2 and Young-Joon Kim1,*

Center for Molecular Medicine, Samsung Biomedical Research Institute, Sungkyunkwan University College of Medicine, Seoul 135-230, Korea,1 and Harvard Microchemistry Facility, Harvard University, Cambridge, Massachusetts 021382

Received 15 September 1998/Returned for modification 26 October 1998/Accepted 3 November 1998

The multisubunit Mediator complex of Saccharomyces cerevisiae is required for most RNA polymerase II (Pol II) transcription. The Mediator complex is composed of two subcomplexes, the Rgr1 and Srb4 subcomplexes, which appear to function in the reception of activator signals and the subsequent modulation of Pol II activity, respectively. In order to determine the precise composition of the Mediator complex and to explore the specific role of each Mediator protein, our goal was to identify all of the Mediator components. To this end, we cloned three previously unidentified Mediator subunits, Med9/Cse2, Med10/Nut2, and Med11, and isolated mutant forms of each of them to analyze their transcriptional defects. Differential display and Northern analyses of mRNAs from wild-type and Mediator mutant cells demonstrated an activator-specific requirement for each Mediator subunit. Med9/Cse2 and Med10/Nut2 were required, respectively, for Bas1/Bas2- and Gcn4-mediated transcription of amino acid biosynthetic genes. Gal11 was required for Gal4- and Rap1-mediated transcriptional activation. Med11 was also required specifically for MFalpha 1 transcription. On the other hand, Med6 was required for all of these transcriptional activation processes. These results suggest that distinct Mediator proteins in the Rgr1 subcomplex are required for activator-specific transcriptional activation and that the activation signals mediated by these Mediator proteins converge on Med6 (or the Srb4 subcomplex) to modulate Pol II activity.

* Corresponding author. Mailing address: Center for Molecular Medicine, Samsung Biomedical Research Institute, Sungkyunkwan University College of Medicine, 50 IIwon-dong, Kangnam-ku, Seoul 135-230, Korea. Phone: 82-2-3410-3638. Fax: 82-2-3410-3649. E-mail: yjkim{at}smc.samsung.co.kr.

dagger Present address: Center for Ligand and Transcription, Chonnam National University, Kwangju 500-757, Korea.

Dagger Present address: Department of Parasitology, College of Medicine, Yonsei University, Seoul 120-752, Korea.

Molecular and Cellular Biology, February 1999, p. 979-988, Vol. 19, No. 2
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Takahashi, H., Kasahara, K., Kokubo, T. (2009). Saccharomyces cerevisiae Med9 comprises two functionally distinct domains that play different roles in transcriptional regulation. GENES CELLS 14: 53-67 [Abstract] [Full Text]  
  • He, Q., Battistella, L., Morse, R. H. (2008). Mediator Requirement Downstream of Chromatin Remodeling during Transcriptional Activation of CHA1 in Yeast. J. Biol. Chem. 283: 5276-5286 [Abstract] [Full Text]  
  • Kim, D.-H., Kim, G. S., Yun, C. H., Lee, Y. C. (2008). Functional Conservation of the Glutamine-Rich Domains of Yeast Gal11 and Human SRC-1 in the Transactivation of Glucocorticoid Receptor Tau 1 in Saccharomyces cerevisiae. Mol. Cell. Biol. 28: 913-925 [Abstract] [Full Text]  
  • Baidoobonso, S. M., Guidi, B. W., Myers, L. C. (2007). Med19(Rox3) Regulates Intermodule Interactions in the Saccharomyces cerevisiae Mediator Complex. J. Biol. Chem. 282: 5551-5559 [Abstract] [Full Text]  
  • Martchenko, M., Levitin, A., Whiteway, M. (2007). Transcriptional Activation Domains of the Candida albicans Gcn4p and Gal4p Homologs. Eukaryot Cell 6: 291-301 [Abstract] [Full Text]  
  • Leroy, C., Cormier, L., Kuras, L. (2006). Independent Recruitment of Mediator and SAGA by the Activator Met4. Mol. Cell. Biol. 26: 3149-3163 [Abstract] [Full Text]  
  • Singh, H., Erkine, A. M., Kremer, S. B., Duttweiler, H. M., Davis, D. A., Iqbal, J., Gross, R. R., Gross, D. S. (2006). A Functional Module of Yeast Mediator That Governs the Dynamic Range of Heat-Shock Gene Expression. Genetics 172: 2169-2184 [Abstract] [Full Text]  
  • Gulshan, K., Rovinsky, S. A., Coleman, S. T., Moye-Rowley, W. S. (2005). Oxidant-specific Folding of Yap1p Regulates Both Transcriptional Activation and Nuclear Localization. J. Biol. Chem. 280: 40524-40533 [Abstract] [Full Text]  
  • Guglielmi, B., van Berkum, N. L., Klapholz, B., Bijma, T., Boube, M., Boschiero, C., Bourbon, H.-M., Holstege, F. C. P., Werner, M. (2004). A high resolution protein interaction map of the yeast Mediator complex. Nucleic Acids Res 32: 5379-5391 [Abstract] [Full Text]  
  • Kim, T. W., Kwon, Y.-J., Kim, J. M., Song, Y.-H., Kim, S. N., Kim, Y.-J. (2004). MED16 and MED23 of Mediator are coactivators of lipopolysaccharide- and heat-shock-induced transcriptional activators. Proc. Natl. Acad. Sci. USA 101: 12153-12158 [Abstract] [Full Text]  
  • Zhang, F., Sumibcay, L., Hinnebusch, A. G., Swanson, M. J. (2004). A Triad of Subunits from the Gal11/Tail Domain of Srb Mediator Is an In Vivo Target of Transcriptional Activator Gcn4p. Mol. Cell. Biol. 24: 6871-6886 [Abstract] [Full Text]  
  • Cantin, G. T., Stevens, J. L., Berk, A. J. (2003). Activation domain-mediator interactions promote transcription preinitiation complex assembly on promoter DNA. Proc. Natl. Acad. Sci. USA 100: 12003-12008 [Abstract] [Full Text]  
  • Lewis, B. A., Reinberg, D. (2003). The mediator coactivator complex: functional and physical roles in transcriptional regulation. J. Cell Sci. 116: 3667-3675 [Abstract] [Full Text]  
  • Park, J. M., Kim, J. M., Kim, L. K., Kim, S. N., Kim-Ha, J., Kim, J. H., Kim, Y.-J. (2003). Signal-Induced Transcriptional Activation by Dif Requires the dTRAP80 Mediator Module. Mol. Cell. Biol. 23: 1358-1367 [Abstract] [Full Text]  
  • Balciunas, D., Hallberg, M., Bjorklund, S., Ronne, H. (2003). Functional Interactions within Yeast Mediator and Evidence of Differential Subunit Modifications. J. Biol. Chem. 278: 3831-3839 [Abstract] [Full Text]  
  • Gauthier, L., Dziak, R., Kramer, D. J. H., Leishman, D., Song, X., Ho, J., Radovic, M., Bentley, D., Yankulov, K. (2002). The Role of the Carboxyterminal Domain of RNA Polymerase II in Regulating Origins of DNA Replication in Saccharomyces cerevisiae. Genetics 162: 1117-1129 [Abstract] [Full Text]  
  • Shim, E. Y., Walker, A. K., Blackwell, T. K. (2002). Broad Requirement for the Mediator Subunit RGR-1 for Transcription in the Caenorhabditis elegans Embryo. J. Biol. Chem. 277: 30413-30416 [Abstract] [Full Text]  
  • Lu, Z., Ansari, A. Z., Lu, X., Ogirala, A., Ptashne, M. (2002). A target essential for the activity of a nonacidic yeast transcriptional activator. Proc. Natl. Acad. Sci. USA 99: 8591-8596 [Abstract] [Full Text]  
  • Hinnebusch, A. G., Natarajan, K. (2002). Gcn4p, a Master Regulator of Gene Expression, Is Controlled at Multiple Levels by Diverse Signals of Starvation and Stress. Eukaryot Cell 1: 22-32 [Full Text]  
  • Kang, J. S., Kim, S. H., Hwang, M. S., Han, S. J., Lee, Y. C., Kim, Y.-J. (2001). The Structural and Functional Organization of the Yeast Mediator Complex. J. Biol. Chem. 276: 42003-42010 [Abstract] [Full Text]  
  • Bhoite, L. T., Yu, Y., Stillman, D. J. (2001). The Swi5 activator recruits the Mediator complex to the HO promoter without RNA polymerase II. Genes Dev. 15: 2457-2469 [Abstract] [Full Text]  
  • Howard, S. C., Chang, Y.-W., Budovskaya, Y. V., Herman, P. K. (2001). The Ras/PKA Signaling Pathway of Saccharomyces cerevisiae Exhibits a Functional Interaction With the Sin4p Complex of the RNA Polymerase II Holoenzyme. Genetics 159: 77-89 [Abstract] [Full Text]  
  • Gim, B. S., Park, J. M., Yoon, J. H., Kang, C., Kim, Y.-J. (2001). Drosophila Med6 Is Required for Elevated Expression of a Large but Distinct Set of Developmentally Regulated Genes. Mol. Cell. Biol. 21: 5242-5255 [Abstract] [Full Text]  
  • Wang, G., Cantin, G. T., Stevens, J. L., Berk, A. J. (2001). Characterization of Mediator Complexes from HeLa Cell Nuclear Extract. Mol. Cell. Biol. 21: 4604-4613 [Abstract] [Full Text]  
  • Chang, Y.-W., Howard, S. C., Budovskaya, Y. V., Rine, J., Herman, P. K. (2001). The rye Mutants Identify a Role for Ssn/Srb Proteins of the RNA Polymerase II Holoenzyme During Stationary Phase Entry in Saccharomyces cerevisiae. Genetics 157: 17-26 [Abstract] [Full Text]  
  • Park, J. M., Kim, H.-S., Han, S. J., Hwang, M.-S., Lee, Y. C., Kim, Y.-J. (2000). In Vivo Requirement of Activator-Specific Binding Targets of Mediator. Mol. Cell. Biol. 20: 8709-8719 [Abstract] [Full Text]  
  • Costa, P. J., Arndt, K. M. (2000). Synthetic Lethal Interactions Suggest a Role for the Saccharomyces cerevisiae Rtf1 Protein in Transcription Elongation. Genetics 156: 535-547 [Abstract] [Full Text]  
  • Kim, S., Cabane, K., Hampsey, M., Reinberg, D. (2000). Genetic Analysis of the Ydr1-Bur6 Repressor Complex Reveals an Intricate Balance among Transcriptional Regulatory Proteins in Yeast. Mol. Cell. Biol. 20: 2455-2465 [Abstract] [Full Text]  
  • Kwon, J. Y., Park, J. M., Gim, B. S., Han, S. J., Lee, J., Kim, Y.-J. (1999). Caenorhabditis elegans Mediator complexes are required for developmental-specific transcriptional activation. Proc. Natl. Acad. Sci. USA 96: 14990-14995 [Abstract] [Full Text]  
  • Lee, D.-k., Kim, S., Lis, J. T. (1999). Different upstream transcriptional activators have distinct coactivator requirements. Genes Dev. 13: 2934-2939 [Abstract] [Full Text]  
  • Yankulov, K., Todorov, I., Romanowski, P., Licatalosi, D., Cilli, K., McCracken, S., Laskey, R., Bentley, D. L. (1999). MCM Proteins Are Associated with RNA Polymerase II Holoenzyme. Mol. Cell. Biol. 19: 6154-6163 [Abstract] [Full Text]  
  • Lee, Y. C., Park, J. M., Min, S., Han, S. J., Kim, Y.-J. (1999). An Activator Binding Module of Yeast RNA Polymerase II Holoenzyme. Mol. Cell. Biol. 19: 2967-2976 [Abstract] [Full Text]  
  • Liu, Y., Ranish, J. A., Aebersold, R., Hahn, S. (2001). Yeast Nuclear Extract Contains Two Major Forms of RNA Polymerase II Mediator Complexes. J. Biol. Chem. 276: 7169-7175 [Abstract] [Full Text]  
  • Han, S. J., Lee, J.-S., Kang, J. S., Kim, Y.-J. (2001). Med9/Cse2 and Gal11 Modules Are Required for Transcriptional Repression of Distinct Group of Genes. J. Biol. Chem. 276: 37020-37026 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»