This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Masumi, A.
Right arrow Articles by Ozato, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Masumi, A.
Right arrow Articles by Ozato, K.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, March 1999, p. 1810-1820, Vol. 19, No. 3
0270-7306/99

The Histone Acetylase PCAF Is a Phorbol-Ester-Inducible Coactivator of the IRF Family That Confers Enhanced Interferon Responsiveness

Atsuko Masumi,dagger I-Ming Wang, Bruno Lefebvre, Xing-Jiao Yang, Yoshihiro Nakatani, and Keiko Ozato*

Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2753

Received 6 July 1998/Returned for modification 14 September 1998/Accepted 19 November 1998

Transcription factors of the interferon regulatory factor (IRF) family bind to the type I interferon (IFN)-responsive element (ISRE) and activate transcription from IFN-inducible genes. To identify cofactors that associate with IRF proteins, DNA affinity binding assays were performed with nuclear extracts prepared from tissue culture cells. The results demonstrated that the endogenous IRFs bound to the ISRE are complexed with the histone acetylases, PCAF, GCN5, and p300/CREB binding protein and that histone acetylase activities are accumulated on the IRF-ISRE complexes. By testing recombinant proteins, we show that PCAF directly binds to some but not all members of the IRF family through distinct domains of the two proteins. This interaction was functionally significant, since transfection of PCAF strongly enhanced IRF-1- and IRF-2-dependent promoter activities. Further studies showed that expression of PCAF and other histone acetylases was markedly induced in U937 cells upon phorbol ester treatment, which led to increased recruitment of PCAF to the IRF-ISRE complexes. Coinciding with the induction of histone acetylases, phorbol ester markedly enhanced IFN-alpha -stimulated gene expression in U937 cells. Supporting the role for PCAF in conferring IFN responsiveness, transfection of PCAF into U937 cells led to a large increase in IFN-alpha -inducible promoter activity. These results demonstrate that PCAF is a phorbol ester-inducible coactivator of the IRF proteins which contributes to the establishment of type I IFN responsiveness.

* Corresponding author. Mailing address: Laboratory of Molecular Growth Regulation, Bldg. 6, Rm. 2A01, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-2735. Phone: (301) 496-9184. Fax: (301) 480-9354. E-mail: ozatok{at}nih.gov.

dagger Present address: National Institute of Infectious Diseases, Tokyo 208, Japan.

Molecular and Cellular Biology, March 1999, p. 1810-1820, Vol. 19, No. 3
0270-7306/99


This article has been cited by other articles:

  • Koeppel, M., van Heeringen, S. J., Smeenk, L., Navis, A. C., Janssen-Megens, E. M., Lohrum, M. (2009). The novel p53 target gene IRF2BP2 participates in cell survival during the p53 stress response. Nucleic Acids Res 37: 322-335 [Abstract] [Full Text]  
  • Matsumura, S., Fujita, Y., Gomez, E., Tanese, N., Wilson, A. C. (2005). Activation of the Kaposi's Sarcoma-Associated Herpesvirus Major Latency Locus by the Lytic Switch Protein RTA (ORF50). J. Virol. 79: 8493-8505 [Abstract] [Full Text]  
  • Matsumura, T., Suzuki, T., Aizawa, K., Munemasa, Y., Muto, S., Horikoshi, M., Nagai, R. (2005). The Deacetylase HDAC1 Negatively Regulates the Cardiovascular Transcription Factor Kruppel-like Factor 5 through Direct Interaction. J. Biol. Chem. 280: 12123-12129 [Abstract] [Full Text]  
  • Chung, M.-C., Kawamoto, S. (2004). IRF-2 Is Involved in Up-regulation of Nonmuscle Myosin Heavy Chain II-A Gene Expression during Phorbol Ester-induced Promyelocytic HL-60 Differentiation. J. Biol. Chem. 279: 56042-56052 [Abstract] [Full Text]  
  • Nguyen, T. L.-A., Calomme, C., Wijmeersch, G., Nizet, S., Veithen, E., Portetelle, D., de Launoit, Y., Burny, A., Van Lint, C. (2004). Deacetylase Inhibitors and the Viral Transactivator TaxBLV Synergistically Activate Bovine Leukemia Virus Gene Expression via a cAMP-responsive Element- and cAMP-responsive Element-binding Protein-dependent Mechanism. J. Biol. Chem. 279: 35025-35036 [Abstract] [Full Text]  
  • Lubyova, B., Kellum, M. J., Frisancho, A. J., Pitha, P. M. (2004). Kaposi's Sarcoma-associated Herpesvirus-encoded vIRF-3 Stimulates the Transcriptional Activity of Cellular IRF-3 and IRF-7. J. Biol. Chem. 279: 7643-7654 [Abstract] [Full Text]  
  • Miyamoto, S., Suzuki, T., Muto, S., Aizawa, K., Kimura, A., Mizuno, Y., Nagino, T., Imai, Y., Adachi, N., Horikoshi, M., Nagai, R. (2003). Positive and Negative Regulation of the Cardiovascular Transcription Factor KLF5 by p300 and the Oncogenic Regulator SET through Interaction and Acetylation on the DNA-Binding Domain. Mol. Cell. Biol. 23: 8528-8541 [Abstract] [Full Text]  
  • Nishikawa, K., Kobayashi, M., Masumi, A., Lyons, S. E., Weinstein, B. M., Liu, P. P., Yamamoto, M. (2003). Self-Association of Gata1 Enhances Transcriptional Activity In Vivo in Zebra Fish Embryos. Mol. Cell. Biol. 23: 8295-8305 [Abstract] [Full Text]  
  • Xie, R.-L., Gupta, S., Miele, A., Shiffman, D., Stein, J. L., Stein, G. S., van Wijnen, A. J. (2003). The Tumor Suppressor Interferon Regulatory Factor 1 Interferes with SP1 Activation to Repress the Human CDK2 Promoter. J. Biol. Chem. 278: 26589-26596 [Abstract] [Full Text]  
  • Masumi, A., Yamakawa, Y., Fukazawa, H., Ozato, K., Komuro, K. (2003). Interferon Regulatory Factor-2 Regulates Cell Growth through Its Acetylation. J. Biol. Chem. 278: 25401-25407 [Abstract] [Full Text]  
  • Childs, K. S., Goodbourn, S. (2003). Identification of novel co-repressor molecules for Interferon Regulatory Factor-2. Nucleic Acids Res 31: 3016-3026 [Abstract] [Full Text]  
  • Suh, N., Roberts, A. B., Birkey Reffey, S., Miyazono, K., Itoh, S., Dijke, P. t., Heiss, E. H., Place, A. E., Risingsong, R., Williams, C. R., Honda, T., Gribble, G. W., Sporn, M. B. (2003). Synthetic Triterpenoids Enhance Transforming Growth Factor {beta}/Smad Signaling. Cancer Res. 63: 1371-1376 [Abstract] [Full Text]  
  • Yamagoe, S., Kanno, T., Kanno, Y., Sasaki, S., Siegel, R. M., Lenardo, M. J., Humphrey, G., Wang, Y., Nakatani, Y., Howard, B. H., Ozato, K. (2003). Interaction of Histone Acetylases and Deacetylases In Vivo. Mol. Cell. Biol. 23: 1025-1033 [Abstract] [Full Text]  
  • Kuwata, T., Gongora, C., Kanno, Y., Sakaguchi, K., Tamura, T., Kanno, T., Basrur, V., Martinez, R., Appella, E., Golub, T., Ozato, K. (2002). Gamma Interferon Triggers Interaction between ICSBP (IRF-8) and TEL, Recruiting the Histone Deacetylase HDAC3 to the Interferon-Responsive Element. Mol. Cell. Biol. 22: 7439-7448 [Abstract] [Full Text]  
  • Shuling Zhang, , Thomas, K., Blanco, J. C.G., Salkowski, C. A., Vogel, S. N. (2002). The role of the interferon regulatory factors, IRF-1 and IRF-2, in LPS-induced cyclooxygenase-2 (COX-2) expression in vivo and in vitro. Innate Immunity 8: 381-390 [Abstract]  
  • Xie, R.-L., van Wijnen, A. J., van der Meijden, C. M., Stein, J. L., Stein, G. S. (2002). Forced Expression of the Interferon Regulatory Factor 2 Oncoprotein Causes Polyploidy and Cell Death in FDC-P1 Myeloid Hematopoietic Progenitor Cells. Cancer Res. 62: 2510-2515 [Abstract] [Full Text]  
  • Lee, D., Hwang, S. G., Kim, J., Choe, J. (2002). Functional Interaction between p/CAF and Human Papillomavirus E2 Protein. J. Biol. Chem. 277: 6483-6489 [Abstract] [Full Text]  
  • Kollet, J., Witek, C., Gentry, J. D., Liu, X., Schwartzbach, S. D., Petro, T. M. (2001). Deletional Analysis of the Murine IL-12 p35 Promoter Comparing IFN-{gamma} and Lipopolysaccharide Stimulation. J. Immunol. 167: 5653-5663 [Abstract] [Full Text]  
  • Au, W.-C., Pitha, P. M. (2001). Recruitment of Multiple Interferon Regulatory Factors and Histone Acetyltransferase to the Transcriptionally Active Interferon A Promoters. J. Biol. Chem. 276: 41629-41637 [Abstract] [Full Text]  
  • Marecki, S., Riendeau, C. J., Liang, M. D., Fenton, M. J. (2001). PU.1 and Multiple IFN Regulatory Factor Proteins Synergize to Mediate Transcriptional Activation of the Human IL-1{{beta}} Gene. J. Immunol. 166: 6829-6838 [Abstract] [Full Text]  
  • Shestakova, E., Bandu, M.-T., Doly, J., Bonnefoy, E. (2001). Inhibition of Histone Deacetylation Induces Constitutive Derepression of the Beta Interferon Promoter and Confers Antiviral Activity. J. Virol. 75: 3444-3452 [Abstract] [Full Text]  
  • Chen, J., Ueda, K., Sakakibara, S., Okuno, T., Parravicini, C., Corbellino, M., Yamanishi, K. (2001). Activation of latent Kaposi's sarcoma-associated herpesvirus by demethylation of the promoter of the lytic transactivator. Proc. Natl. Acad. Sci. USA 98: 4119-4124 [Abstract] [Full Text]  
  • Spilianakis, C., Papamatheakis, J., Kretsovali, A. (2000). Acetylation by PCAF Enhances CIITA Nuclear Accumulation and Transactivation of Major Histocompatibility Complex Class II Genes. Mol. Cell. Biol. 20: 8489-8498 [Abstract] [Full Text]  
  • Li, M., Damania, B., Alvarez, X., Ogryzko, V., Ozato, K., Jung, J. U. (2000). Inhibition of p300 Histone Acetyltransferase by Viral Interferon Regulatory Factor. Mol. Cell. Biol. 20: 8254-8263 [Abstract] [Full Text]  
  • Wang, I-M., Contursi, C., Masumi, A., Ma, X., Trinchieri, G., Ozato, K. (2000). An IFN-{gamma}-Inducible Transcription Factor, IFN Consensus Sequence Binding Protein (ICSBP), Stimulates IL-12 p40 Expression in Macrophages. J. Immunol. 165: 271-279 [Abstract] [Full Text]  
  • Blanco, J. C. G., Contursi, C., Salkowski, C. A., DeWitt, D. L., Ozato, K., Vogel, S. N. (2000). Interferon Regulatory Factor (Irf)-1 and Irf-2 Regulate Interferon {gamma}-Dependent Cyclooxygenase 2 Expression. JEM 191: 2131-2144 [Abstract] [Full Text]  
  • Li, X.-L., Blackford, J. A., Judge, C. S., Liu, M., Xiao, W., Kalvakolanu, D. V., Hassel, B. A. (2000). RNase-L-dependent Destabilization of Interferon-induced mRNAs. A ROLE FOR THE 2-5A SYSTEM IN ATTENUATION OF THE INTERFERON RESPONSE. J. Biol. Chem. 275: 8880-8888 [Abstract] [Full Text]  
  • Huang, S., Brandt, S. J. (2000). mSin3A Regulates Murine Erythroleukemia Cell Differentiation through Association with the TAL1 (or SCL) Transcription Factor. Mol. Cell. Biol. 20: 2248-2259 [Abstract] [Full Text]  
  • Yeow, W.-S., Au, W.-C., Juang, Y.-T., Fields, C. D., Dent, C. L., Gewert, D. R., Pitha, P. M. (2000). Reconstitution of Virus-mediated Expression of Interferon alpha Genes in Human Fibroblast Cells by Ectopic Interferon Regulatory Factor-7. J. Biol. Chem. 275: 6313-6320 [Abstract] [Full Text]  
  • Contursi, C., Wang, I-M., Gabriele, L., Gadina, M., O'Shea, J., Morse, H. C. III, Ozato, K. (2000). IFN consensus sequence binding protein potentiates STAT1-dependent activation of IFNgamma -responsive promoters in macrophages. Proc. Natl. Acad. Sci. USA 97: 91-96 [Abstract] [Full Text]  
  • Meraro, D., Hashmueli, S., Koren, B., Azriel, A., Oumard, A., Kirchhoff, S., Hauser, H., Nagulapalli, S., Atchison, M. L., Levi, B.-Z. (1999). Protein-Protein and DNA-Protein Interactions Affect the Activity of Lymphoid-Specific IFN Regulatory Factors. J. Immunol. 163: 6468-6478 [Abstract] [Full Text]  
  • Jayachandra, S., Low, K. G., Thlick, A.-E., Yu, J., Ling, P. D., Chang, Y., Moore, P. S. (1999). Three unrelated viral transforming proteins (vIRF, EBNA2, and E1A) induce the MYC oncogene through the interferon-responsive PRF element by using different transcription coadaptors. Proc. Natl. Acad. Sci. USA 96: 11566-11571 [Abstract] [Full Text]  
  • Kurooka, H., Honjo, T. (2000). Functional Interaction between the Mouse Notch1 Intracellular Region and Histone Acetyltransferases PCAF and GCN5. J. Biol. Chem. 275: 17211-17220 [Abstract] [Full Text]  
  • Xie, R., van Wijnen, A. J., van der Meijden, C., Luong, M. X., Stein, J. L., Stein, G. S. (2001). The Cell Cycle Control Element of Histone H4 Gene Transcription Is Maximally Responsive to Interferon Regulatory Factor Pairs IRF-1/IRF-3 and IRF-1/IRF-7. J. Biol. Chem. 276: 18624-18632 [Abstract] [Full Text]  
  • Masumi, A., Ozato, K. (2001). Coactivator p300 Acetylates the Interferon Regulatory Factor-2 in U937 Cells following Phorbol Ester Treatment. J. Biol. Chem. 276: 20973-20980 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»