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Molecular and Cellular Biology, March 1999, p. 2118-2129, Vol. 19, No. 3
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

SWM1, a Developmentally Regulated Gene, Is Required for Spore Wall Assembly in Saccharomyces cerevisiae

Sandra Ufano, Pedro San-Segundo,dagger Francisco del Rey, and Carlos R. Vázquez de Aldana*

Departamento de Microbiología y Genética, Instituto de Microbiología-Bioquímica, Universidad de Salamanca/CSIC, Campus Miguel de Unamuno, 37007 Salamanca, Spain

Received 2 October 1998/Returned for modification 11 November 1998/Accepted 7 December 1998

Meiosis in Saccharomyces cerevisiae is followed by encapsulation of haploid nuclei within multilayered spore walls. Formation of this spore-specific wall requires the coordinated activity of enzymes involved in the biosynthesis of its components. Completion of late events in the sporulation program, leading to spore wall formation, requires the SWM1 gene. SWM1 is expressed at low levels during vegetative growth but its transcription is strongly induced under sporulating conditions, with kinetics similar to those of middle sporulation-specific genes. Homozygous swm1Delta diploids proceed normally through both meiotic divisions but fail to produce mature asci. Consistent with this finding, swm1Delta mutant asci display enhanced sensitivity to enzymatic digestion and heat shock. Deletion of SWM1 specifically affects the expression of mid-late and late sporulation-specific genes. All of the phenotypes observed are similar to those found for the deletion of SPS1 or SMK1, two putative components of a sporulation-specific MAP kinase cascade. However, epistasis analyses indicate that Swm1p does not form part of the Sps1p-Smk1p-MAP kinase pathway. We propose that Swm1p, a nuclear protein, would participate in a different signal transduction pathway that is also required for the coordination of the biochemical and morphological events occurring during the last phase of the sporulation program.

* Corresponding author. Mailing address: Departamento de Microbiología y Genética, Instituto de Microbiología-Bioquímica, Universidad de Salamanca/CSIC, Campus Miguel de Unamuno, 37007 Salamanca, Spain. Phone: (34) 923-294675. Fax: (34) 923-224876. E-mail: cvazquez{at}www-micro.usal.es.

dagger Present address: Howard Hughes Medical Institute, Yale University, New Haven, CT 06520-8103.

Molecular and Cellular Biology, March 1999, p. 2118-2129, Vol. 19, No. 3
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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