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Molecular and Cellular Biology, March 1999, p. 2220-2230, Vol. 19, No. 3
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Mammalian Staufen Is a Double-Stranded-RNA- and Tubulin-Binding Protein Which Localizes to the Rough Endoplasmic Reticulum

Louise Wickham,1 Thomas Duchaîne,1 Ming Luo,1 Ivan R. Nabi,2 and Luc DesGroseillers1,*

Departments of Biochemistry1 and Pathology and Cell Biology,2 University of Montreal, Montreal, Quebec, Canada H3C 3J7

Received 9 September 1998/Returned for modification 23 October 1998/Accepted 17 November 1998

Staufen (Stau) is a double-stranded RNA (dsRNA)-binding protein involved in mRNA transport and localization in Drosophila. To understand the molecular mechanisms of mRNA transport in mammals, we cloned human (hStau) and mouse (mStau) staufen cDNAs. In humans, four transcripts arise by differential splicing of the Stau gene and code for two proteins with different N-terminal extremities. In vitro, hStau and mStau bind dsRNA via each of two full-length dsRNA-binding domains and tubulin via a region similar to the microtubule-binding domain of MAP-1B, suggesting that Stau cross-links cytoskeletal and RNA components. Immunofluorescent double labeling of transfected mammalian cells revealed that Stau is localized to the rough endoplasmic reticulum (RER), implicating this RNA-binding protein in mRNA targeting to the RER, perhaps via a multistep process involving microtubules. These results are the first demonstration of the association of an RNA-binding protein in addition to ribosomal proteins, with the RER, implicating this class of proteins in the transport of RNA to its site of translation.

* Corresponding author mailing address: Department of Biochemistry, University of Montreal, P.O. Box 6128, Station Centre Ville, Montreal, Quebec, Canada H3C 3J7. Phone: (514) 343-5802. Fax: (514) 343-2210. E-mail: desgros{at}bcm.umontreal.ca.

Molecular and Cellular Biology, March 1999, p. 2220-2230, Vol. 19, No. 3
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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