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Molecular and Cellular Biology, March 1999, p. 2308-2321, Vol. 19, No. 3
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Down-Regulation of RpS21, a Putative Translation Initiation Factor Interacting with P40, Produces Viable Minute Imagos and Larval Lethality with Overgrown Hematopoietic Organs and Imaginal Discs

István Török,1 Daniela Herrmann-Horle,1 István Kiss,2 Gabriela Tick,2 Gábor Speer,1,dagger Rolf Schmitt,1 and Bernard M. Mechler1,*

Department of Developmental Genetics, Deutsches Krebsforschungszentrum, D-69120 Heidelberg, Germany,1 and Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, H-6701 Szeged, Hungary2

Received 27 August 1998/Returned for modification 27 October 1998/Accepted 7 December 1998

Down-regulation of the Drosophila ribosomal protein S21 gene (rpS21) causes a dominant weak Minute phenotype and recessively produces massive hyperplasia of the hematopoietic organs and moderate overgrowth of the imaginal discs during larval development. Here, we show that the S21 protein (RpS21) is bound to native 40S ribosomal subunits in a salt-labile association and is absent from polysomes, indicating that it acts as a translation initiation factor rather than as a core ribosomal protein. RpS21 can interact strongly with P40, a ribosomal peripheral protein encoded by the stubarista (sta) gene. Genetic studies reveal that P40 underexpression drastically enhances imaginal disc overgrowth in rpS21-deficient larvae, whereas viable combinations between rpS21 and sta affect the morphology of bristles, antennae, and aristae. These data demonstrate a strong interaction between components of the translation machinery and showed that their underexpression impairs the control of cell proliferation in both hematopoietic organs and imaginal discs.

* Corresponding author. Mailing address: Department of Developmental Genetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany. Phone: 49-6221-424502. Fax: 49-6221-424552. E-mail: dev.genetics{at}dkfz-heidelberg.de.

dagger Present address: First Department of Medicine, Semmelweis University Medical School, H-1083 Budapest, Hungary.

Molecular and Cellular Biology, March 1999, p. 2308-2321, Vol. 19, No. 3
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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