This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Török, I.
Right arrow Articles by Mechler, B. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Török, I.
Right arrow Articles by Mechler, B. M.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, March 1999, p. 2308-2321, Vol. 19, No. 3
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Down-Regulation of RpS21, a Putative Translation Initiation Factor Interacting with P40, Produces Viable Minute Imagos and Larval Lethality with Overgrown Hematopoietic Organs and Imaginal Discs

István Török,1 Daniela Herrmann-Horle,1 István Kiss,2 Gabriela Tick,2 Gábor Speer,1,dagger Rolf Schmitt,1 and Bernard M. Mechler1,*

Department of Developmental Genetics, Deutsches Krebsforschungszentrum, D-69120 Heidelberg, Germany,1 and Institute of Genetics, Biological Research Center of the Hungarian Academy of Sciences, H-6701 Szeged, Hungary2

Received 27 August 1998/Returned for modification 27 October 1998/Accepted 7 December 1998

Down-regulation of the Drosophila ribosomal protein S21 gene (rpS21) causes a dominant weak Minute phenotype and recessively produces massive hyperplasia of the hematopoietic organs and moderate overgrowth of the imaginal discs during larval development. Here, we show that the S21 protein (RpS21) is bound to native 40S ribosomal subunits in a salt-labile association and is absent from polysomes, indicating that it acts as a translation initiation factor rather than as a core ribosomal protein. RpS21 can interact strongly with P40, a ribosomal peripheral protein encoded by the stubarista (sta) gene. Genetic studies reveal that P40 underexpression drastically enhances imaginal disc overgrowth in rpS21-deficient larvae, whereas viable combinations between rpS21 and sta affect the morphology of bristles, antennae, and aristae. These data demonstrate a strong interaction between components of the translation machinery and showed that their underexpression impairs the control of cell proliferation in both hematopoietic organs and imaginal discs.

* Corresponding author. Mailing address: Department of Developmental Genetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany. Phone: 49-6221-424502. Fax: 49-6221-424552. E-mail: dev.genetics{at}dkfz-heidelberg.de.

dagger Present address: First Department of Medicine, Semmelweis University Medical School, H-1083 Budapest, Hungary.

Molecular and Cellular Biology, March 1999, p. 2308-2321, Vol. 19, No. 3
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Perreault, A., Gascon, S., D'Amours, A., Aletta, J. M., Bachand, F. (2009). A Methyltransferase-independent Function for Rmt3 in Ribosomal Subunit Homeostasis. J. Biol. Chem. 284: 15026-15037 [Abstract] [Full Text]  
  • Malygin, A. A., Parakhnevitch, N. M., Ivanov, A. V., Eperon, I. C., Karpova, G. G. (2007). Human ribosomal protein S13 regulates expression of its own gene at the splicing step by a feedback mechanism. Nucleic Acids Res 35: 6414-6423 [Abstract] [Full Text]  
  • Liu, W., Silverstein, A. M., Shu, H., Martinez, B., Mumby, M. C. (2007). A Functional Genomics Analysis of the B56 Isoforms of Drosophila Protein Phosphatase 2A. Mol. Cell. Proteomics 6: 319-332 [Abstract] [Full Text]  
  • Bachand, F., Lackner, D. H., Bahler, J., Silver, P. A. (2006). Autoregulation of ribosome biosynthesis by a translational response in fission yeast.. Mol. Cell. Biol. 26: 1731-1742 [Abstract] [Full Text]  
  • Coelho, C. M. A., Kolevski, B., Walker, C. D., Lavagi, I., Shaw, T., Ebert, A., Leevers, S. J., Marygold, S. J. (2005). A Genetic Screen for Dominant Modifiers of a Small-Wing Phenotype in Drosophila melanogaster Identifies Proteins Involved in Splicing and Translation. Genetics 171: 597-614 [Abstract] [Full Text]  
  • Anne, J., Mechler, B. M. (2005). Valois, a component of the nuage and pole plasm, is involved in assembly of these structures, and binds to Tudor and the methyltransferase Capsuleen. Development 132: 2167-2177 [Abstract] [Full Text]  
  • Schulze, S. R., Sinclair, D. A. R., Fitzpatrick, K. A., Honda, B. M. (2005). A Genetic and Molecular Characterization of Two Proximal Heterochromatic Genes on Chromosome 3 of Drosophila melanogaster. Genetics 169: 2165-2177 [Abstract] [Full Text]  
  • Marygold, S. J., Coelho, C. M. A., Leevers, S. J. (2005). Genetic Analysis of RpL38 and RpL5, Two Minute Genes Located in the Centric Heterochromatin of Chromosome 2 of Drosophila melanogaster. Genetics 169: 683-695 [Abstract] [Full Text]  
  • Matsson, H., Davey, E. J., Draptchinskaia, N., Hamaguchi, I., Ooka, A., Leveen, P., Forsberg, E., Karlsson, S., Dahl, N. (2004). Targeted Disruption of the Ribosomal Protein S19 Gene Is Lethal Prior to Implantation. Mol. Cell. Biol. 24: 4032-4037 [Abstract] [Full Text]  
  • Tabb-Massey, A., Caffrey, J. M., Logsden, P., Taylor, S., Trent, J. O., Ellis, S. R. (2003). Ribosomal proteins Rps0 and Rps21 of Saccharomyces cerevisiae have overlapping functions in the maturation of the 3' end of 18S rRNA. Nucleic Acids Res 31: 6798-6805 [Abstract] [Full Text]  
  • Kunert, N., Marhold, J., Stanke, J., Stach, D., Lyko, F. (2003). A Dnmt2-like protein mediates DNA methylation in Drosophila. Development 130: 5083-5090 [Abstract] [Full Text]  
  • Lankenau, S., Barnickel, T., Marhold, J., Lyko, F., Mechler, B. M., Lankenau, D.-H. (2003). Knockout Targeting of the Drosophila Nap1 Gene and Examination of DNA Repair Tracts in the Recombination Products. Genetics 163: 611-623 [Abstract] [Full Text]  
  • Schenkel, H., Hanke, S., De Lorenzo, C., Schmitt, R., Mechler, B. M. (2002). P Elements Inserted in the Vicinity of or Within the Drosophila snRNP SmD3 Gene Nested in the First Intron of the Ornithine Decarboxylase Antizyme Gene Affect Only the Expression of SmD3. Genetics 161: 763-772 [Abstract] [Full Text]  
  • Aoyagi, N., Wassarman, D. A. (2001). Developmental and Transcriptional Consequences of Mutations in Drosophila TAFII60. Mol. Cell. Biol. 21: 6808-6819 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»