This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Berry, L. D.
Right arrow Articles by Gould, K. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Berry, L. D.
Right arrow Articles by Gould, K. L.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, April 1999, p. 2535-2546, Vol. 19, No. 4
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The Schizosaccharomyces pombe dim1+ Gene Interacts with the Anaphase-Promoting Complex or Cyclosome (APC/C) Component lid1+ and Is Required for APC/C Function

Lynne D. Berry,1 Anna Feoktistova,2 Melanie D. Wright,1 and Kathleen L. Gould1,2,*

Howard Hughes Medical Institute2 and Department of Cell Biology,1 Vanderbilt University School of Medicine, Nashville, Tennessee 37232

Received 5 August 1998/Returned for modification 21 September 1998/Accepted 22 December 1998

The Schizosaccharomyces pombe dim1+ gene is required for entry into mitosis and for chromosome segregation during mitosis. To further understand dim1p function, we undertook a synthetic lethal screen with the temperature-sensitive dim1-35 mutant and isolated lid (for lethal in dim1-35) mutants. Here, we describe the temperature-sensitive lid1-6 mutant. At the restrictive temperature of 36°C, lid1-6 mutant cells arrest with a "cut" phenotype similar to that of cut4 and cut9 mutants. An epitope-tagged version of lid1p is a component of a multiprotein ~20S complex; the presence of lid1p in this complex depends upon functional cut9+. lid1p-myc coimmunoprecipitates with several other proteins, including cut9p and nuc2p, and the presence of cut9p in a 20S complex depends upon the activity of lid1+. Further, lid1+ function is required for the multiubiquitination of cut2p, an anaphase-promoting complex or cyclosome (APC/C) target. Thus, lid1p is a component of the S. pombe APC/C. In dim1 mutants, the abundances of lid1p and the APC/C complex decline significantly, and the ubiquitination of an APC/C target is abolished. These data suggest that at least one role of dim1p is to maintain or establish the steady-state level of the APC/C.

* Corresponding author. Mailing address: HHMI and Dept. of Cell Biology, Vanderbilt University School of Medicine, Nashville, TN 37232. Phone: (615) 343-9502. Fax: (615) 343-0723. E-mail: kathy.gould{at}mcmail.vanderbilt.edu.

Molecular and Cellular Biology, April 1999, p. 2535-2546, Vol. 19, No. 4
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Yoon, H.-J., Feoktistova, A., Chen, J.-S., Jennings, J. L., Link, A. J., Gould, K. L. (2006). Role of Hcn1 and Its Phosphorylation in Fission Yeast Anaphase-promoting Complex/Cyclosome Function. J. Biol. Chem. 281: 32284-32293 [Abstract] [Full Text]  
  • Ren, L., Feoktistova, A., McDonald, W. H., Haese, G. D., Morrell, J. L., Gould, K. L. (2005). Analysis of the Role of Phosphorylation in Fission Yeast Cdc13p/CyclinB Function. J. Biol. Chem. 280: 14591-14596 [Abstract] [Full Text]  
  • Carnahan, R. H., Feoktistova, A., Ren, L., Niessen, S., Yates, J. R. III, Gould, K. L. (2005). Dim1p Is Required for Efficient Splicing and Export of mRNA Encoding Lid1p, a Component of the Fission Yeast Anaphase-Promoting Complex. Eukaryot Cell 4: 577-587 [Abstract] [Full Text]  
  • Tarayre, S., Vinardell, J. M., Cebolla, A., Kondorosi, A., Kondorosi, E. (2004). Two Classes of the Cdh1-Type Activators of the Anaphase-Promoting Complex in Plants: Novel Functional Domains and Distinct Regulation. Plant Cell 16: 422-434 [Abstract] [Full Text]  
  • Yen, H.-C. S., Espiritu, C., Chang, E. C. (2003). Rpn5 Is a Conserved Proteasome Subunit and Required for Proper Proteasome Localization and Assembly. J. Biol. Chem. 278: 30669-30676 [Abstract] [Full Text]  
  • Tasto, J. J., Morrell, J. L., Gould, K. L. (2003). An anillin homologue, Mid2p, acts during fission yeast cytokinesis to organize the septin ring and promote cell separation. JCB 160: 1093-1103 [Abstract] [Full Text]  
  • Chang, L., Morrell, J. L., Feoktistova, A., Gould, K. L. (2001). Study of Cyclin Proteolysis in Anaphase-Promoting Complex (APC) Mutant Cells Reveals the Requirement for APC Function in the Final Steps of the Fission Yeast Septation Initiation Network. Mol. Cell. Biol. 21: 6681-6694 [Abstract] [Full Text]  
  • Blanco, M. A., Pelloquin, L., Moreno, S. (2001). Fission yeast mfr1 activates APC and coordinates meiotic nuclear division with sporulation. J. Cell Sci. 114: 2135-2143 [Abstract] [Full Text]  
  • Furuta, T., Tuck, S., Kirchner, J., Koch, B., Auty, R., Kitagawa, R., Rose, A. M., Greenstein, D. (2000). EMB-30: An APC4 Homologue Required for Metaphase-to-Anaphase Transitions during Meiosis and Mitosis in Caenorhabditis elegans. Mol. Biol. Cell 11: 1401-1419 [Abstract] [Full Text]  
  • ZHANG, Y.-Z., GOULD, K. L., DUNBRACK, R. L. JR., CHENG, H., RODER, H., GOLEMIS, E. A. (1999). The evolutionarily conserved Dim1 protein defines a novel branch of the thioredoxin fold superfamily. Physiol. Genomics 1: 109-118 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»