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Molecular and Cellular Biology, April 1999, p. 2594-2600, Vol. 19, No. 4
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
The c-fos Proto-Oncogene Is a Target for
Transactivation by the p53 Tumor Suppressor
Adi
Elkeles,
Tamar
Juven-Gershon,
David
Israeli,
Sylvia
Wilder,
Amir
Zalcenstein, and
Moshe
Oren*
Department of Molecular Cell Biology, The
Weizmann Institute of Science, Rehovot 76100, Israel
Received 26 October 1998/Returned for modification 21 December
1998/Accepted 6 January 1999
The p53 tumor suppressor gene is mutated in over 50% of human
cancers, resulting in inactivation of the wild-type (wt) p53 protein. The most notable biochemical feature of p53 is its ability to
act as a sequence-specific transcriptional activator. Through use
of the suppression subtractive hybridization differential screening
technique, we identified c-fos as a target for
transcriptional stimulation by p53 in cells undergoing
p53-mediated apoptosis. Overexpression of wt p53 induces
c-fos mRNA and protein. Moreover, in vivo induction of
c-fos in the thymus following whole-body exposure to
ionizing radiation is p53 dependent. p53 responsiveness does not reside
in the basal c-fos promoter. Rather, a distinct region
within the c-fos gene first intron binds
specifically to p53 and confers upon the c-fos promoter
the ability to become transcriptionally activated by wt p53.
Identification of c-fos as a specific target for
transcriptional activation by p53 establishes a direct link between
these two pivotal regulatory proteins and raises the possibility that
c-fos contributes to some of the biological effects of p53.
*
Corresponding author. Mailing address: Department
of Molecular Cell Biology, The Weizmann Institute of Science,
Rehovot 76100, Israel. Phone: (972) 8-9342358. Fax: (972)
8-9465223. E-mail: lioren{at}dapsas1.weizmann.ac.il.
Molecular and Cellular Biology, April 1999, p. 2594-2600, Vol. 19, No. 4
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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