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Molecular and Cellular Biology, April 1999, p. 2967-2976, Vol. 19, No. 4
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

An Activator Binding Module of Yeast RNA Polymerase II Holoenzyme

Young Chul Lee,dagger Jin Mo Park, Soyoung Min, Sang Jun Han, and Young-Joon Kim*

Center for Molecular Medicine, Samsung Biomedical Research Institute, Sungkyunkwan University College of Medicine, Kangnam-ku, Seoul 135-230, Korea

Received 4 September 1998/Returned for modification 27 October 1998/Accepted 11 January 1999

The Mediator complex of Saccharomyces cerevisiae is required for both general and regulated transcription of RNA polymerase II (PolII) and is composed of two stable subcomplexes (Srb4 and Rgr1 subcomplexes). To decipher the function of each Mediator subcomplex and to delineate the functional relationship between the subcomplexes, we characterized the compositions and biochemical activities of PolII-Mediator complexes (holoenzymes) prepared from several Mediator mutant strains of S. cerevisiae. We found that holoenzymes devoid of a functional Gal11 module were defective for activated but not basal transcription in a reconstituted in vitro system. This activation-specific defect was correlated with a crippled physical interaction to transcriptional activator proteins, which could be bypassed by artificial recruitment of a mutant holoenzyme to a promoter. Consistent with this observation, a direct interaction between Gal11 and gene-specific transcriptional activator proteins was detected by far-Western analyses and column binding assays. In contrast, the srb5 deletion mutant holoenzyme was defective for both basal and activated transcription, despite its capacity for activator binding that is comparable to that of the wild-type holoenzyme. These results demonstrate that the Gal11 module of the Rgr1 subcomplex is required for the efficient recruitment of PolII holoenzyme to a promoter via activator-specific interactions, while the Srb4 subcomplex functions in the modulation of general polymerase activity.

* Corresponding author. Mailing address: Center for Molecular Medicine, Samsung Biomedical Research Institute, Sungkyunkwan University College of Medicine, 50 Ilwong-dong, Kangnam-ku, Seoul 135-230, Korea. Phone: 82-2-3410-3638. Fax: 82-2-3410-3649. E-mail: yjkim{at}smc.samsung.co.kr.

dagger Present address: Center for Ligand and Transcription, Chonnam National University, 300 Yongbong-dong, Puk-ku, Kwangju 500-757, Korea.

Molecular and Cellular Biology, April 1999, p. 2967-2976, Vol. 19, No. 4
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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