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Molecular and Cellular Biology, May 1999, p. 3360-3371, Vol. 19, No. 5
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Transcriptional Induction by Aromatic Amino Acids
in Saccharomyces cerevisiae
Ismaïl
Iraqui,
Stéphan
Vissers,
Bruno
André, and
Antonio
Urrestarazu*
Laboratoire de Physiologie Cellulaire et de
Génétique des Levures, Université Libre de
Bruxelles
Campus Plaine CP244, B-1050 Brussels, Belgium
Received 20 July 1998/Returned for modification 30 September
1998/Accepted 15 February 1999
Aromatic aminotransferase II, product of the ARO9 gene,
catalyzes the first step of tryptophan, phenylalanine, and tyrosine catabolism in Saccharomyces cerevisiae. ARO9 expression is
under the dual control of specific induction and nitrogen source
regulation. We have here identified UASaro, a 36-bp
upstream element necessary and sufficient to promote transcriptional
induction of reporter gene expression in response to tryptophan,
phenylalanine, or tyrosine. We then isolated mutants in which
UASaro-mediated ARO9 transcription is
partially or totally impaired. Mutations abolishing
ARO9 induction affect a gene called ARO80
(YDR421w), coding for a
Zn2Cys6 family transcription factor. A sequence
highly similar to UASaro was found upstream from the
YDR380w gene encoding a homolog of bacterial indolepyruvate decarboxylase. In yeast, this enzyme is postulated to catalyze the second step of tryptophan catabolism to
tryptophol. We show that ARO9 and YDR380w
(named ARO10) have similar patterns of transcriptional
regulation and are both under the positive control of Aro80p. Nitrogen
regulation of ARO9 expression seems not directly to involve
the general factor Ure2p, Gln3p, Nil1p, Uga43p, or Gzf3p.
ARO9 expression appears, rather, to be mainly regulated by
inducer exclusion. Finally, we show that Gap1p, the general amino acid
permease, and Wap1p (Ycl025p), a newly discovered inducible amino
acid permease with broad specificity, are the main aromatic amino
acid transporters for catabolic purposes.
*
Corresponding author. Mailing address: Laboratoire de
Physiologie Cellulaire et de Génétique des Levures,
Université de Bruxelles
Campus Plaine CP244, Bd du Triomphe,
B-1050 Brussels, Belgium. Phone: 32-2-650-5428. Fax: 32-2-650-5421. E-mail: laurrest{at}ulb.ac.be.
Molecular and Cellular Biology, May 1999, p. 3360-3371, Vol. 19, No. 5
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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