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Molecular and Cellular Biology, May 1999, p. 3443-3456, Vol. 19, No. 5
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

The su(Hw) Insulator Can Disrupt Enhancer-Promoter Interactions When Located More than 20 Kilobases Away from the Drosophila achaete-scute Complex

Anton Golovnin,1,2 Maria Gause,1 Sofia Georgieva,1,2,3 Elena Gracheva,1 and Pavel Georgiev1,*

Department of the Control of Genetic Processes, Institute of Gene Biology,1 and Biomedical Center of Oslo University Institute of Gene Biology,2 Russian Academy of Sciences, Moscow 117334, and Engelghardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow 117984,3 Russia

Received 5 June 1998/Returned for modification 19 August 1998/Accepted 19 January 1999

Here we report that the su(Hw) insulator may not necessarily separate promoters from enhancers to allow inhibition of transcription by the su(Hw) protein. For this purpose we used the strains of Drosophila melanogaster which carry inversion of the region containing the yellow gene and the achaete-scute complex (AS-C). Despite the reverse orientation of the region, the AS-C enhancers continue to activate achaete and scute gene expression. The su(Hw) insulator, located more than 20 kb away from the inversion, facilitates strong suppression of achaete and scute gene expression, although is does not separate the promoters from the AS-C enhancers.

* Corresponding author. Mailing address: Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., Moscow 117334, Russia. Phone: 7-095-1359734. Fax: 7-095-1354105. E-mail: pgeorg{at}biogen.msk.su.

Molecular and Cellular Biology, May 1999, p. 3443-3456, Vol. 19, No. 5
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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