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Molecular and Cellular Biology, May 1999, p. 3588-3599, Vol. 19, No. 5
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Yeast Mutants Affecting Possible Quality Control of Plasma Membrane Proteins

Yu Li, Thomas Kane, Christopher Tipper, Phyllis Spatrick, and Duane D. Jenness*

Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655-0122

Received 21 September 1998/Returned for modification 10 November 1998/Accepted 30 January 1999

Mutations gef1, stp22, STP26, and STP27 in Saccharomyces cerevisiae were identified as suppressors of the temperature-sensitive alpha -factor receptor (mutation ste2-3) and arginine permease (mutation can1ts). These suppressors inhibited the elimination of misfolded receptors (synthesized at 34°C) as well as damaged surface receptors (shifted from 22 to 34°C). The stp22 mutation (allelic to vps23 [M. Babst and S. Emr, personal communication] and the STP26 mutation also caused missorting of carboxypeptidase Y, and ste2-3 was suppressed by mutations vps1, vps8, vps10, and vps28 but not by mutation vps3. In the stp22 mutant, both the mutant and the wild-type receptors (tagged with green fluorescent protein [GFP]) accumulated within an endosome-like compartment and were excluded from the vacuole. GFP-tagged Stp22p also accumulated in this compartment. Upon reaching the vacuole, cytoplasmic domains of both mutant and wild-type receptors appeared within the vacuolar lumen. Stp22p and Gef1p are similar to tumor susceptibility protein TSG101 and voltage-gated chloride channel, respectively. These results identify potential elements of plasma membrane quality control and indicate that cytoplasmic domains of membrane proteins are translocated into the vacuolar lumen.

* Corresponding author. Mailing address: Department MGM, University of Massachusetts Medical School, Worcester, MA 01655-0122. Phone: (508) 856-2157. Fax: (508) 856-5920. E-mail: Duane.Jenness{at}ummed.edu.

Molecular and Cellular Biology, May 1999, p. 3588-3599, Vol. 19, No. 5
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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