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Molecular and Cellular Biology, May 1999, p. 3600-3606, Vol. 19, No. 5
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Identification of the Novel Player
EF1 in
Estrogen Transcriptional Cascades
Elaine M.
Chamberlain and
Michel M.
Sanders*
Department of Biochemistry, Molecular
Biology, and Biophysics, University of Minnesota, Minneapolis,
Minnesota
Received 19 January 1999/Accepted 26 February 1999
Although many genes are regulated by estrogen, very few have been
shown to directly bind the estrogen receptor complex. Therefore, transcriptional cascades probably occur in which the estrogen receptor
directly binds to a target gene that encodes another transcription
factor that subsequently regulates additional genes. Through the use of
a differential display assay, a transcription factor has been
identified that may be involved in estrogen transcriptional cascades.
This report demonstrates that transcription factor
EF1 is induced
eightfold by estrogen in the chick oviduct. Furthermore, the regulation
by estrogen occurs at the transcriptional level and is likely to be a
direct effect of the estrogen receptor complex, as it does not require
concomitant protein synthesis. A putative binding site was identified
in the 5'-flanking region of the chick ovalbumin gene identifying it as
a possible target gene for regulation by
EF1. Characterization of
this binding site revealed that
EF1 binds to and regulates the chick
ovalbumin gene. Thus, a novel regulatory cascade that is triggered by
estrogen has been defined.
*
Corresponding author. Mailing address: Department of
Biochemistry, Molecular Biology, and Biophysics, University of
Minnesota, 435 Delaware St. SE, Minneapolis, MN 55455. Phone: (612)
624-9637. Fax: (612) 625-2163. E-mail:
sande001{at}tc.umn.edu.
Molecular and Cellular Biology, May 1999, p. 3600-3606, Vol. 19, No. 5
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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