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Molecular and Cellular Biology, May 1999, p. 3736-3747, Vol. 19, No. 5
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Activation of Somatostatin Receptor II Expression by Transcription Factors MIBP1 and SEF-2 in the Murine Brain

Ulrike Dörflinger,1 Armin Pscherer,2 Markus Moser,3 Petra Rümmele,2 Roland Schüle,1 and Reinhard Buettner2,*

Institut für Experimentelle Krebsforschung, Klinik für Tumorbiologie an der Universität Freiburg, D-79106 Freiburg,1 Institut für Pathologie, Universität Regensburg, D-93042 Regensburg,2 and Institut für Pathologie, Klinikum der RWTH Aachen, D-52074 Aachen,3 Germany

Received 5 November 1998/Returned for modification 30 November 1998/Accepted 2 February 1999

Somatostatin receptor type II expression in the mammalian brain displays a spatially and temporally very restricted pattern. In an investigation of the molecular mechanisms controlling these patterns, we have recently shown that binding of the transcription factor SEF-2 to a novel initiator element in the SSTR-2 promoter is essential for SSTR-2 gene expression. Further characterization of the promoter identified a species-conserved TC-rich enhancer element. By screening a mouse brain cDNA expression library, we cloned a cDNA encoding the transcription factor MIBP1. MIBP1 interacts specifically with both the TC box in the SSTR-2 promoter and with the SEF-2 initiator-binding protein to enhance transcription from the basal SSTR-2 promoter. We then investigated SSTR-2, SEF-2, and MIBP1 mRNA expression patterns in the developing and adult murine brain by Northern blotting and in situ hybridization. While SEF-2 is widely expressed in many neuronal and nonneuronal tissues, MIBP1 expression overlapped precisely with expression of SSTR-2 in the frontal cortex and hippocampus. In summary, our data for the first time define a regulatory role for the transcription factor MIBP1 in mediating spatially and temporally regulated SSTR-2 expression in the brain.

* Corresponding author. Present address: Institute for Pathology, University Hospital, RWTH Aachen, Pauwelsstrasse 30, D-52074 Aachen, Germany. Phone: (49) 241-8089281. Fax: (49) 241-8888439. E-mail: Buettner{at}pat.rwth-aachen.de.

Molecular and Cellular Biology, May 1999, p. 3736-3747, Vol. 19, No. 5
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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