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Molecular and Cellular Biology, May 1999, p. 3769-3778, Vol. 19, No. 5
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

TAFII40 Protein Is Encoded by the e(y)1 Gene: Biological Consequences of Mutations

Aleksei Soldatov,1,2,3 Elena Nabirochkina,1,3 Sofia Georgieva,1,2,4 Tatiana Belenkaja,1,2 and Pavel Georgiev1,3,*

Department of the Control of Genetic Processes1 and Centre for Medical Research of Oslo University,2 Institute of Gene Biology, and Engelhardt Institute of Molecular Biology,4 Russian Academy of Sciences, Russia, and International Centre of Genetic Engineering and Biotechnology, Trieste, Italy3

Received 1 December 1997/Returned for modification 20 January 1998/Accepted 13 January 1999

The enhancer of yellow 1 gene, e(y)1, of Drosophila melanogaster has been cloned and demonstrated to encode the TAFII40 protein. The e(y)1 gene is expressed in females much more strongly than in males due to the accumulation of e(y)1 mRNA in the ovaries. Two different e(y)1 mutations have been obtained. The e(y)1ul mutation, induced by the insertion of Stalker into the coding region, leads to the replacement of 25 carboxy-terminal amino acids by 17 amino acids encoded by the Stalker sequences and to a decrease of the e(y)1 transcription level. The latter is the main cause of dramatic underdevelopment of the ovaries and sterility of females bearing the e(y)1 mutation. This follows from the restoration of female fertility upon transformation of e(y)1u1 flies with a construction synthesizing the mutant protein. The e(y)1P1 mutation induced by P element insertion into the transcribed nontranslated region of the gene has almost no influence on the phenotype of flies. However, in combination with the phP1 mutation, which leads to a strong P element-mediated suppression of e(y)1 transcription, this mutation is lethal. Genetic studies of the e(y)1u1 mutation revealed a sensitivity of the yellow and white expression to the TAFII40/e(y)1 level. The su(Hw)-binding region, Drosophila insulator, stabilizes the expression of the white gene and makes it independent of the e(y)1u1 mutation.

* Corresponding author. Mailing address: Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., Moscow 117334, Russia. Phone: 7-095-1359734. Fax: 7-095-1354105. E-mail: pgeorg{at}biogen.msk.su.

Molecular and Cellular Biology, May 1999, p. 3769-3778, Vol. 19, No. 5
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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