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Molecular and Cellular Biology, May 1999, p. 3904-3915, Vol. 19, No. 5
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Sequence-Independent Assembly of Spermatid mRNAs into Messenger Ribonucleoprotein Particles

Edward E. Schmidt,1,* Eric S. Hanson,2 and Mario R. Capecchi1,*

Howard Hughes Medical Institute1 and Eccles Program in Human Molecular Biology and Genetics,2 Eccles Institute of Human Genetics, University of Utah, Salt Lake City, Utah 84112

Received 22 December 1998/Accepted 8 February 1999

During mammalian spermatogenesis, meiosis is followed by a brief period of high transcriptional activity. At this time a large amount of mRNA is stored as messenger ribonucleoprotein (mRNP) particles. All subsequent processes of sperm maturation occur in the complete absence of transcription, primarily using proteins which are newly synthesized from these stored mRNAs. By expressing transgene mRNAs in the early haploid spermatids of mice, we have investigated the sequence requirements for determining whether specific mRNAs in these cells will be stored as mRNP particles or be assembled into polysomes. The results suggest that mRNAs which are transcribed in spermatids are assembled into mRNP particles by a mechanism that acts independently of mRNA sequence. Our findings reveal a fundamental similarity between the mechanisms of translational control used in spermatogenesis and oogenesis.

* Corresponding author. Mailing address: Howard Hughes Medical Institute, 5400 Eccles Institute of Human Genetics, University of Utah, Salt Lake City, UT 84112. Phone: (801) 581-7097. Fax: (801) 585-3425. E-mail for Edward E. Schmidt: eschmidt{at}howard.genetics.utah.edu.

Molecular and Cellular Biology, May 1999, p. 3904-3915, Vol. 19, No. 5
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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