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Molecular and Cellular Biology, June 1999, p. 4516-4524, Vol. 19, No. 6
0270-7306/99/$04.00+0
Two Prion-Inducing Regions of Ure2p Are
Nonoverlapping
Marie-Lise
Maddelein and
Reed B.
Wickner*
Laboratory of Biochemistry and Genetics,
National Institute of Diabetes Digestive and Kidney Diseases,
National Institutes of Health, Bethesda, Maryland 20892-0830
Received 13 January 1999/Returned for modification 2 March
1999/Accepted 9 March 1999
Ure2p of Saccharomyces cerevisiae normally functions in
blocking utilization of a poor nitrogen source when a good nitrogen source is available. The non-Mendelian genetic element [URE3] is a
prion (infectious protein) form of Ure2p, so that overexpression of
Ure2p induces the de novo appearance of infectious [URE3]. Earlier
studies defined a prion domain comprising Ure2p residues 1 to 64 and a
nitrogen regulation domain included in residues 66 to 354. We find that
deletion of individual runs of asparagine within the prion domain
reduce prion-inducing activity. Although residues 1 to 64 are
sufficient for prion induction, the fragment from residues 1 to 80 is a
more efficient inducer of [URE3]. In-frame deletion of a region
around residue 224 does not affect nitrogen regulation but does
eliminate prion induction by the remainder of Ure2p. Larger deletions
removing the region around residue 224 and more of the C-terminal part
of Ure2p restore prion-inducing ability. A fragment of Ure2p lacking
the original prion domain does not induce [URE3], but surprisingly,
further deletion of residues 151 to 157 and 348 to 354 leaves a
fragment that can do so. The region from 66 to 80 and the region around
residue 224 are both necessary for this second prion-inducing activity. Thus, each of two nonoverlapping parts of Ure2p is sufficient to induce
the appearance of the [URE3] prion.
*
Corresponding author. Mailing address: Bldg. 8, Room
225, NIH, 8 Center Dr. MSC 0830, Bethesda, MD 20892-0830. Phone: (301) 496-3452. Fax: (301) 402-0240. E-mail:
wickner{at}helix.nih.gov.
Molecular and Cellular Biology, June 1999, p. 4516-4524, Vol. 19, No. 6
0270-7306/99/$04.00+0
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