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Molecular and Cellular Biology, July 1999, p. 4552-4560, Vol. 19, No. 7
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

An mRNA Stability Complex Functions with Poly(A)-Binding Protein To Stabilize mRNA In Vitro

Zuoren Wang, Nancy Day, Panayiota Trifillis, and Megerditch Kiledjian*

Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, New Jersey 08854-8082

Received 8 February 1999/Returned for modification 8 March 1999/Accepted 7 April 1999

The stable globin mRNAs provide an ideal system for studying the mechanism governing mammalian mRNA turnover. alpha -Globin mRNA stability is dictated by sequences in the 3' untranslated region (3'UTR) which form a specific ribonucleoprotein complex (alpha -complex) whose presence correlates with mRNA stability. One of the major protein components within this complex is a family of two polycytidylate-binding proteins, alpha CP1 and alpha CP2. Using an in vitro-transcribed and polyadenylated alpha -globin 3'UTR, we have devised an in vitro mRNA decay assay which reproduces the alpha -complex-dependent mRNA stability observed in cells. Incubation of the RNA with erythroleukemia K562 cytosolic extract results in deadenylation with distinct intermediates containing a periodicity of approximately 30 nucleotides, which is consistent with the binding of poly(A)-binding protein (PABP) monomers. Disruption of the alpha -complex by sequestration of alpha CP1 and alpha CP2 enhances deadenylation and decay of the mRNA, while reconstitution of the alpha -complex stabilizes the mRNA. Similarly, PABP is also essential for the stability of mRNA in vitro, since rapid deadenylation resulted upon its depletion. An RNA-dependent interaction between alpha CP1 and alpha CP2 with PABP suggests that the alpha -complex can directly interact with PABP. Therefore, the alpha -complex is an mRNA stability complex in vitro which could function at least in part by interacting with PABP.

* Corresponding author. Mailing address: Rutgers University, Department of Cell Biology and Neuroscience, 604 Allison Rd., Piscataway, NJ 08854-8082. Phone: (732) 445-0796. Fax: (732) 445-5870. E-mail: kiledjia{at}biology.rutgers.edu.

Molecular and Cellular Biology, July 1999, p. 4552-4560, Vol. 19, No. 7
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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