This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ho, Y.
Right arrow Articles by Andrews, B. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ho, Y.
Right arrow Articles by Andrews, B. J.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, August 1999, p. 5267-5278, Vol. 19, No. 8
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Regulation of Transcription at the Saccharomyces cerevisiae Start Transition by Stb1, a Swi6-Binding Protein

Yuen Ho,1 Michael Costanzo,1 Lynda Moore,1 Ryuji Kobayashi,2 and Brenda J. Andrews1,*

Department of Molecular and Medical Genetics, University of Toronto, Toronto, Canada M5S 1A8,1 and Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 117242

Received 19 March 1999/Returned for modification 28 April 1999/Accepted 30 April 1999

In Saccharomyces cerevisiae, gene expression in the late G1 phase is activated by two transcription factors, SBF and MBF. SBF contains the Swi4 and Swi6 proteins and activates the transcription of G1 cyclin genes, cell wall biosynthesis genes, and the HO gene. MBF is composed of Mbp1 and Swi6 and activates the transcription of genes required for DNA synthesis. Mbp1 and Swi4 are the DNA binding subunits for MBF and SBF, while the common subunit, Swi6, is presumed to play a regulatory role in both complexes. We show that Stb1, a protein first identified in a two-hybrid screen with the transcriptional repressor Sin3, binds Swi6 in vitro. The STB1 transcript was cell cycle periodic and peaked in late G1 phase. In vivo accumulation of Stb1 phosphoforms was dependent on CLN1, CLN2, and CLN3, which encode G1-specific cyclins for the cyclin-dependent kinase Cdc28, and Stb1 was phosphorylated by Cln-Cdc28 kinases in vitro. Deletion of STB1 caused an exacerbated delay in G1 progression and the onset of Start transcription in a cln3Delta strain. Our results suggest a role for STB1 in controlling the timing of Start transcription that is revealed in the absence of the G1 regulator CLN3, and they implicate Stb1 as an in vivo target of G1-specific cyclin-dependent kinases.

* Corresponding author. Mailing address: Department of Molecular and Medical Genetics, University of Toronto, Rm. 4285, Medical Sciences Building, 1 Kings College Circle, Toronto, Ontario, Canada M5S 1A8. Phone: (416) 978-8562. Fax: (416) 978-6885. E-mail: brenda.andrews{at}utoronto.ca.

Molecular and Cellular Biology, August 1999, p. 5267-5278, Vol. 19, No. 8
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Scrimale, T., Didone, L., de Mesy Bentley, K. L., Krysan, D. J. (2009). The Unfolded Protein Response Is Induced by the Cell Wall Integrity Mitogen-activated Protein Kinase Signaling Cascade and Is Required for Cell Wall Integrity in Saccharomyces cerevisiae. Mol. Biol. Cell 20: 164-175 [Abstract] [Full Text]  
  • de Bruin, R. A. M., Kalashnikova, T. I., Wittenberg, C. (2008). Stb1 Collaborates with Other Regulators To Modulate the G1-Specific Transcriptional Circuit. Mol. Cell. Biol. 28: 6919-6928 [Abstract] [Full Text]  
  • Ashe, M., de Bruin, R. A. M., Kalashnikova, T., McDonald, W. H., Yates, J. R. III, Wittenberg, C. (2008). The SBF- and MBF-associated Protein Msa1 Is Required for Proper Timing of G1-specific Transcription in Saccharomyces cerevisiae. J. Biol. Chem. 283: 6040-6049 [Abstract] [Full Text]  
  • Chang, Y.-H., Wang, Y.-C., Chen, B.-S. (2006). Identification of transcription factor cooperativity via stochastic system model. Bioinformatics 22: 2276-2282 [Abstract] [Full Text]  
  • Tsai, H.-K., Lu, H. H.-S., Li, W.-H. (2005). Statistical methods for identifying yeast cell cycle transcription factors. Proc. Natl. Acad. Sci. USA 102: 13532-13537 [Abstract] [Full Text]  
  • Bean, J. M., Siggia, E. D., Cross, F. R. (2005). High Functional Overlap Between MluI Cell-Cycle Box Binding Factor and Swi4/6 Cell-Cycle Box Binding Factor in the G1/S Transcriptional Program in Saccharomyces cerevisiae. Genetics 171: 49-61 [Abstract] [Full Text]  
  • Nagamine, N., Kawada, Y., Sakakibara, Y. (2005). Identifying cooperative transcriptional regulations using protein-protein interactions. Nucleic Acids Res 33: 4828-4837 [Abstract] [Full Text]  
  • Geymonat, M., Spanos, A., Wells, G. P., Smerdon, S. J., Sedgwick, S. G. (2004). Clb6/Cdc28 and Cdc14 Regulate Phosphorylation Status and Cellular Localization of Swi6. Mol. Cell. Biol. 24: 2277-2285 [Abstract] [Full Text]  
  • Banerjee, N., Zhang, M. Q. (2003). Identifying cooperativity among transcription factors controlling the cell cycle in yeast. Nucleic Acids Res 31: 7024-7031 [Abstract] [Full Text]  
  • Yeger-Lotem, E., Margalit, H. (2003). Detection of regulatory circuits by integrating the cellular networks of protein-protein interactions and transcription regulation. Nucleic Acids Res 31: 6053-6061 [Abstract] [Full Text]  
  • Costanzo, M., Schub, O., Andrews, B. (2003). G1 Transcription Factors Are Differentially Regulated in Saccharomyces cerevisiae by the Swi6-Binding Protein Stb1. Mol. Cell. Biol. 23: 5064-5077 [Abstract] [Full Text]  
  • Sidorova, J. M., Breeden, L. L. (2003). Rad53 Checkpoint Kinase Phosphorylation Site Preference Identified in the Swi6 Protein of Saccharomyces cerevisiae. Mol. Cell. Biol. 23: 3405-3416 [Abstract] [Full Text]  
  • Lee, T. I., Rinaldi, N. J., Robert, F., Odom, D. T., Bar-Joseph, Z., Gerber, G. K., Hannett, N. M., Harbison, C. T., Thompson, C. M., Simon, I., Zeitlinger, J., Jennings, E. G., Murray, H. L., Gordon, D. B., Ren, B., Wyrick, J. J., Tagne, J.-B., Volkert, T. L., Fraenkel, E., Gifford, D. K., Young, R. A. (2002). Transcriptional Regulatory Networks in Saccharomyces cerevisiae. Science 298: 799-804 [Abstract] [Full Text]  
  • Porter, S. E., Washburn, T. M., Chang, M., Jaehning, J. A. (2002). The Yeast Paf1-RNA Polymerase II Complex Is Required for Full Expression of a Subset of Cell Cycle-Regulated Genes. Eukaryot Cell 1: 830-842 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»