This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Klasson, H.
Right arrow Articles by Ljungdahl, P. O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Klasson, H.
Right arrow Articles by Ljungdahl, P. O.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, August 1999, p. 5405-5416, Vol. 19, No. 8
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Ssy1p and Ptr3p Are Plasma Membrane Components of a Yeast System That Senses Extracellular Amino Acids

Hanna Klasson,1 Gerald R. Fink,2 and Per O. Ljungdahl1,*

Ludwig Institute for Cancer Research, S-171 77 Stockholm, Sweden1 and Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 021422

Received 4 January 1999/Returned for modification 22 February 1999/Accepted 4 May 1999

Mutations in SSY1 and PTR3 were identified in a genetic selection for components required for the proper uptake and compartmentalization of histidine in Saccharomyces cerevisiae. Ssy1p is a unique member of the amino acid permease gene family, and Ptr3p is predicted to be a hydrophilic protein that lacks known functional homologs. Both Ssy1p and Ptr3p have previously been implicated in relaying signals regarding the presence of extracellular amino acids. We have found that ssy1 and ptr3 mutants belong to the same epistasis group; single and ssy1 ptr3 double-mutant strains exhibit indistinguishable phenotypes. Mutations in these genes cause the nitrogen-regulated general amino acid permease gene (GAP1) to be abnormally expressed and block the nonspecific induction of arginase (CAR1) and the peptide transporter (PTR2). ssy1 and ptr3 mutations manifest identical differential effects on the functional expression of multiple specific amino acid transporters. ssy1 and ptr3 mutants have increased vacuolar pools of histidine and arginine and exhibit altered cell growth morphologies accompanied by exaggerated invasive growth. Subcellular fractionation experiments reveal that both Ssy1p and Ptr3p are localized to the plasma membrane (PM). Ssy1p requires the endoplasmic reticulum protein Shr3p, the amino acid permease-specific packaging chaperonin, to reach the PM, whereas Ptr3p does not. These findings suggest that Ssy1p and Ptr3p function in the PM as components of a sensor of extracellular amino acids.

* Corresponding author. Mailing address: Ludwig Institute for Cancer Research, Box 240, S-171 77 Stockholm, Sweden. Phone: 46 8 728 7108. Fax: 46 8 33 28 12. E-mail: plju{at}licr.ki.se.

Molecular and Cellular Biology, August 1999, p. 5405-5416, Vol. 19, No. 8
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Thevelein, J. M., Voordeckers, K. (2009). Functioning and Evolutionary Significance of Nutrient Transceptors. Mol Biol Evol 26: 2407-2414 [Abstract] [Full Text]  
  • Darlyuk, I., Goldman, A., Roberts, S. C., Ullman, B., Rentsch, D., Zilberstein, D. (2009). Arginine Homeostasis and Transport in the Human Pathogen Leishmania donovani. J. Biol. Chem. 284: 19800-19807 [Abstract] [Full Text]  
  • Shin, C.-S., Kim, S. Y., Huh, W.-K. (2009). TORC1 controls degradation of the transcription factor Stp1, a key effector of the SPS amino-acid-sensing pathway in Saccharomyces cerevisiae. J. Cell Sci. 122: 2089-2099 [Abstract] [Full Text]  
  • Hundal, H. S., Taylor, P. M. (2009). Amino acid transceptors: gate keepers of nutrient exchange and regulators of nutrient signaling. Am. J. Physiol. Endocrinol. Metab. 296: E603-E613 [Abstract] [Full Text]  
  • Apostolaki, A., Erpapazoglou, Z., Harispe, L., Billini, M., Kafasla, P., Kizis, D., Penalva, M. A., Scazzocchio, C., Sophianopoulou, V. (2009). AgtA, the Dicarboxylic Amino Acid Transporter of Aspergillus nidulans, Is Concertedly Down-Regulated by Exquisite Sensitivity to Nitrogen Metabolite Repression and Ammonium-Elicited Endocytosis. Eukaryot Cell 8: 339-352 [Abstract] [Full Text]  
  • Dabas, N., Schneider, S., Morschhauser, J. (2009). Mutational Analysis of the Candida albicans Ammonium Permease Mep2p Reveals Residues Required for Ammonium Transport and Signaling. Eukaryot Cell 8: 147-160 [Abstract] [Full Text]  
  • Xia, Z., Turner, G. C., Hwang, C.-S., Byrd, C., Varshavsky, A. (2008). Amino Acids Induce Peptide Uptake via Accelerated Degradation of CUP9, the Transcriptional Repressor of the PTR2 Peptide Transporter. J. Biol. Chem. 283: 28958-28968 [Abstract] [Full Text]  
  • Liu, Z., Thornton, J., Spirek, M., Butow, R. A. (2008). Activation of the SPS Amino Acid-Sensing Pathway in Saccharomyces cerevisiae Correlates with the Phosphorylation State of a Sensor Component, Ptr3. Mol. Cell. Biol. 28: 551-563 [Abstract] [Full Text]  
  • Cai, H., Hauser, M., Naider, F., Becker, J. M. (2007). Differential Regulation and Substrate Preferences in Two Peptide Transporters of Saccharomyces cerevisiae. Eukaryot Cell 6: 1805-1813 [Abstract] [Full Text]  
  • Boban, M., Ljungdahl, P. O. (2007). Dal81 Enhances Stp1- and Stp2-Dependent Transcription Necessitating Negative Modulation by Inner Nuclear Membrane Protein Asi1 in Saccharomyces cerevisiae. Genetics 176: 2087-2097 [Abstract] [Full Text]  
  • Godard, P., Urrestarazu, A., Vissers, S., Kontos, K., Bontempi, G., van Helden, J., Andre, B. (2007). Effect of 21 Different Nitrogen Sources on Global Gene Expression in the Yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 27: 3065-3086 [Abstract] [Full Text]  
  • Kota, J., Melin-Larsson, M., Ljungdahl, P. O., Forsberg, H. (2007). Ssh4, Rcr2 and Rcr1 Affect Plasma Membrane Transporter Activity in Saccharomyces cerevisiae. Genetics 175: 1681-1694 [Abstract] [Full Text]  
  • Vargas, R. C., Garcia-Salcedo, R., Tenreiro, S., Teixeira, M. C., Fernandes, A. R., Ramos, J., Sa-Correia, I. (2007). Saccharomyces cerevisiae Multidrug Resistance Transporter Qdr2 Is Implicated in Potassium Uptake, Providing a Physiological Advantage to Quinidine-Stressed Cells. Eukaryot Cell 6: 134-142 [Abstract] [Full Text]  
  • Zargari, A., Boban, M., Heessen, S., Andreasson, C., Thyberg, J., Ljungdahl, P. O. (2007). Inner Nuclear Membrane Proteins Asi1, Asi2, and Asi3 Function in Concert to Maintain the Latent Properties of Transcription Factors Stp1 and Stp2. J. Biol. Chem. 282: 594-605 [Abstract] [Full Text]  
  • Kim, S.-K., Bissati, K. E., Mamoun, C. B. (2006). Amino acids mediate colony and cell differentiation in the fungal pathogen Candida parapsilosis.. Microbiology 152: 2885-2894 [Abstract] [Full Text]  
  • Wu, B., Ottow, K., Poulsen, P., Gaber, R. F., Albers, E., Kielland-Brandt, M. C. (2006). Competitive intra- and extracellular nutrient sensing by the transporter homologue Ssy1p. JCB 173: 327-331 [Abstract] [Full Text]  
  • Kingsbury, J. M., Goldstein, A. L., McCusker, J. H. (2006). Role of Nitrogen and Carbon Transport, Regulation, and Metabolism Genes for Saccharomyces cerevisiae Survival In Vivo. Eukaryot Cell 5: 816-824 [Abstract] [Full Text]  
  • Daniel, H., Spanier, B., Kottra, G., Weitz, D. (2006). From Bacteria to Man: Archaic Proton-Dependent Peptide Transporters at Work. Physiology 21: 93-102 [Abstract] [Full Text]  
  • Poulsen, P., Lo Leggio, L., Kielland-Brandt, M. C. (2006). Mapping of an Internal Protease Cleavage Site in the Ssy5p Component of the Amino Acid Sensor of Saccharomyces cerevisiae and Functional Characterization of the Resulting Pro- and Protease Domains by Gain-of-Function Genetics. Eukaryot Cell 5: 601-608 [Abstract] [Full Text]  
  • Eckert-Boulet, N., Larsson, K., Wu, B., Poulsen, P., Regenberg, B., Nielsen, J., Kielland-Brandt, M. C. (2006). Deletion of RTS1, Encoding a Regulatory Subunit of Protein Phosphatase 2A, Results in Constitutive Amino Acid Signaling via Increased Stp1p Processing. Eukaryot Cell 5: 174-179 [Abstract] [Full Text]  
  • Martinez, P., Ljungdahl, P. O. (2005). Divergence of Stp1 and Stp2 Transcription Factors in Candida albicans Places Virulence Factors Required for Proper Nutrient Acquisition under Amino Acid Control. Mol. Cell. Biol. 25: 9435-9446 [Abstract] [Full Text]  
  • Aouida, M., Leduc, A., Poulin, R., Ramotar, D. (2005). AGP2 Encodes the Major Permease for High Affinity Polyamine Import in Saccharomyces cerevisiae. J. Biol. Chem. 280: 24267-24276 [Abstract] [Full Text]  
  • Poulsen, P., Wu, B., Gaber, R. F., Kielland-Brandt, M. C. (2005). Constitutive Signal Transduction by Mutant Ssy5p and Ptr3p Components of the SPS Amino Acid Sensor System in Saccharomyces cerevisiae. Eukaryot Cell 4: 1116-1124 [Abstract] [Full Text]  
  • Maidan, M. M., De Rop, L., Serneels, J., Exler, S., Rupp, S., Tournu, H., Thevelein, J. M., Van Dijck, P. (2005). The G Protein-coupled Receptor Gpr1 and the G{alpha} Protein Gpa2 Act through the cAMP-Protein Kinase A Pathway to Induce Morphogenesis in Candida albicans. Mol. Biol. Cell 16: 1971-1986 [Abstract] [Full Text]  
  • Abdel-Sater, F., El Bakkoury, M., Urrestarazu, A., Vissers, S., Andre, B. (2004). Amino Acid Signaling in Yeast: Casein Kinase I and the Ssy5 Endoprotease Are Key Determinants of Endoproteolytic Activation of the Membrane-Bound Stp1 Transcription Factor. Mol. Cell. Biol. 24: 9771-9785 [Abstract] [Full Text]  
  • Spielewoy, N., Flick, K., Kalashnikova, T. I., Walker, J. R., Wittenberg, C. (2004). Regulation and Recognition of SCFGrr1 Targets in the Glucose and Amino Acid Signaling Pathways. Mol. Cell. Biol. 24: 8994-9005 [Abstract] [Full Text]  
  • Andreasson, C., Ljungdahl, P. O. (2004). The N-Terminal Regulatory Domain of Stp1p Is Modular and, Fused to an Artificial Transcription Factor, Confers Full Ssy1p-Ptr3p-Ssy5p Sensor Control. Mol. Cell. Biol. 24: 7503-7513 [Abstract] [Full Text]  
  • Abdel-Sater, F., Iraqui, I., Urrestarazu, A., Andre, B. (2004). The External Amino Acid Signaling Pathway Promotes Activation of Stp1 and Uga35/Dal81 Transcription Factors for Induction of the AGP1 Gene in Saccharomyces cerevisiae. Genetics 166: 1727-1739 [Abstract] [Full Text]  
  • Brega, E., Zufferey, R., Mamoun, C. B. (2004). Candida albicans Csy1p Is a Nutrient Sensor Important for Activation of Amino Acid Uptake and Hyphal Morphogenesis. Eukaryot Cell 3: 135-143 [Abstract] [Full Text]  
  • Guaragnella, N., Butow, R. A. (2003). ATO3 Encoding a Putative Outward Ammonium Transporter Is an RTG-independent Retrograde Responsive Gene Regulated by GCN4 and the Ssy1-Ptr3-Ssy5 Amino Acid Sensor System. J. Biol. Chem. 278: 45882-45887 [Abstract] [Full Text]  
  • Santiago, T. C., Mamoun, C. B. (2003). Genome Expression Analysis in Yeast Reveals Novel Transcriptional Regulation by Inositol and Choline and New Regulatory Functions for Opi1p, Ino2p, and Ino4p. J. Biol. Chem. 278: 38723-38730 [Abstract] [Full Text]  
  • Gaber, R. F., Ottow, K., Andersen, H. A., Kielland-Brandt, M. C. (2003). Constitutive and Hyperresponsive Signaling by Mutant Forms of Saccharomyces cerevisiae Amino Acid Sensor Ssy1. Eukaryot Cell 2: 922-929 [Abstract] [Full Text]  
  • Bolton, E. C., Boeke, J. D. (2003). Transcriptional Interactions Between Yeast tRNA Genes, Flanking Genes and Ty Elements: A Genomic Point of View. Genome Res 13: 254-263 [Abstract] [Full Text]  
  • Andreasson, C., Ljungdahl, P. O. (2002). Receptor-mediated endoproteolytic activation of two transcription factors in yeast. Genes Dev. 16: 3158-3172 [Abstract] [Full Text]  
  • Chen, E. J., Kaiser, C. A. (2002). Amino acids regulate the intracellular trafficking of the general amino acid permease of Saccharomycescerevisiae. Proc. Natl. Acad. Sci. USA 99: 14837-14842 [Abstract] [Full Text]  
  • Palmer, L. K., Wolfe, D., Keeley, J. L., Keil, R. L. (2002). Volatile Anesthetics Affect Nutrient Availability in Yeast. Genetics 161: 563-574 [Abstract] [Full Text]  
  • Palecek, S. P., Parikh, A. S., Kron, S. J. (2002). Sensing, signalling and integrating physical processes during Saccharomyces cerevisiae invasive and filamentous growth. Microbiology 148: 893-907 [Full Text]  
  • Bertram, P. G., Choi, J. H., Carvalho, J., Chan, T.-F., Ai, W., Zheng, X. F. S. (2002). Convergence of TOR-Nitrogen and Snf1-Glucose Signaling Pathways onto Gln3. Mol. Cell. Biol. 22: 1246-1252 [Abstract] [Full Text]  
  • Anthony, T. G., Reiter, A. K., Anthony, J. C., Kimball, S. R., Jefferson, L. S. (2001). Deficiency of dietary EAA preferentially inhibits mRNA translation of ribosomal proteins in liver of meal-fed rats. Am. J. Physiol. Endocrinol. Metab. 281: E430-E439 [Abstract] [Full Text]  
  • Forsberg, H., Hammar, M., Andreasson, C., Moliner, A., Ljungdahl, P. O. (2001). Suppressors of ssy1 and ptr3 Null Mutations Define Novel Amino Acid Sensor-Independent Genes in Saccharomyces cerevisiae. Genetics 158: 973-988 [Abstract] [Full Text]  
  • Forsberg, H., Ljungdahl, P. O. (2001). Genetic and Biochemical Analysis of the Yeast Plasma Membrane Ssy1p-Ptr3p-Ssy5p Sensor of Extracellular Amino Acids. Mol. Cell. Biol. 21: 814-826 [Abstract] [Full Text]  
  • Gilstring, C. F., Ljungdahl, P. O. (2000). A Method for Determining the in Vivo Topology of Yeast Polytopic Membrane Proteins Demonstrates That Gap1p Fully Integrates into the Membrane Independently of Shr3p. J. Biol. Chem. 275: 31488-31495 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»