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Molecular and Cellular Biology, September 1999, p. 5952-5959, Vol. 19, No. 9
Karolinska Institute,
Received 18 February 1999/Returned for modification 15 April
1999/Accepted 18 June 1999
Previous studies have shown that the Ada adapter proteins are
important for glucocorticoid receptor (GR)-mediated gene activation in
yeast. The N-terminal transactivation domain of GR,
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Histone Acetyltransferase Complexes Can Mediate
Transcriptional Activation by the Major Glucocorticoid Receptor
Activation Domain
1, is dependent
upon Ada2, Ada3, and Gcn5 for transactivation in vitro and in vivo.
Using in vitro techniques, we demonstrate that the GR-
1 interacts
directly with the native Ada containing histone acetyltransferase (HAT)
complex SAGA but not the related Ada complex. Mutations in
1 that
reduce
1 transactivation activity in vivo lead to a reduced binding
of
1 to the SAGA complex and conversely, mutations increasing the
transactivation activity of
1 lead to an increased binding of
1
to SAGA. In addition, the Ada-independent NuA4 HAT complex also
interacts with
1. GAL4-
1-driven transcription from chromatin
templates is stimulated by SAGA and NuA4 in an acetyl coenzyme
A-dependent manner. Low-activity
1 mutants reduce SAGA- and
NuA4-stimulated transcription while high-activity
1 mutants increase
transcriptional activation, specifically from chromatin templates. Our
results demonstrate that the targeting of native HAT complexes by the
GR-
1 activation domain mediates transcriptional stimulation from
chromatin templates.
*
Corresponding author. Mailing address: Karolinska
Institute, Department of Biosciences, NOVUM, S-14157 Huddinge, Sweden.
Phone: 46-8-6089155. Fax: 46-8-7745538. E-mail:
annika.wallberg{at}csb.ki.se.
Molecular and Cellular Biology, September 1999, p. 5952-5959, Vol. 19, No. 9
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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