Tat-SF1 Protein Associates with RAP30 and Human SPT5 Proteins

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Kim, J. B.
	Articles by Sharp, P. A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Kim, J. B.
	Articles by Sharp, P. A.

Molecular and Cellular Biology, September 1999, p. 5960-5968, Vol. 19, No. 9
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Tat-SF1 Protein Associates with RAP30 and Human SPT5 Proteins

Jae B. Kim,¹ Yuki Yamaguchi,² Tadashi Wada,² Hiroshi Handa,² and Phillip A. Sharp¹^,^*

Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139,¹ and Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-Ku, Yokohama 226-8501, Japan²

Received 5 April 1999/Returned for modification 17 May 1999/Accepted 9 June 1999

The potent transactivator Tat recognizes the transactivation response RNA element (TAR) of human immunodeficiency virus type1 and stimulates the processivity of elongation of RNA polymerase(Pol) II complexes. The cellular proteins Tat-SF1 and human SPT5(hSPT5) are required for Tat activation as shown by immunodepletionwith specific sera and complementation with recombinant proteins.In nuclear extracts, small fractions of both hSPT5 and Pol IIare associated with Tat-SF1 protein. Surprisingly, the RAP30 proteinof the heterodimeric transcription TFIIF factor is associatedwith Tat-SF1, while the RAP74 subunit of TFIIF is not coimmunoprecipitatedwith Tat-SF1. Overexpression of Tat-SF1 and hSPT5 specificallystimulates the transcriptional activity of Tat in vivo. Theseresults suggest that Tat-SF1 and hSPT5 are indispensable cellularfactors supporting Tat-specific transcription activation and thatthey may interact with RAP30 in controllingelongation.

^* Corresponding author. Mailing address: Center for Cancer Research, Massachusetts Institute of Technology, 77 MassachusettsAve., Cambridge, MA 02139. Phone: (617) 253-6421. Fax: (617) 253-3867.E-mail: sharppa{at}mit.edu.

Molecular and Cellular Biology, September 1999, p. 5960-5968, Vol. 19, No. 9
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

Zhou, Q., Yik, J. H. N. (2006). The Yin and Yang of P-TEFb Regulation: Implications for Human Immunodeficiency Virus Gene Expression and Global Control of Cell Growth and Differentiation. Microbiol. Mol. Biol. Rev. 70: 646-659 [Abstract] [Full Text]
Wu, C.-H., Yamaguchi, Y., Benjamin, L. R., Horvat-Gordon, M., Washinsky, J., Enerly, E., Larsson, J., Lambertsson, A., Handa, H., Gilmour, D. (2003). NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila. Genes Dev. 17: 1402-1414 [Abstract] [Full Text]
Narita, T., Yamaguchi, Y., Yano, K., Sugimoto, S., Chanarat, S., Wada, T., Kim, D.-k., Hasegawa, J., Omori, M., Inukai, N., Endoh, M., Yamada, T., Handa, H. (2003). Human Transcription Elongation Factor NELF: Identification of Novel Subunits and Reconstitution of the Functionally Active Complex. Mol. Cell. Biol. 23: 1863-1873 [Abstract] [Full Text]
Lindstrom, D. L., Squazzo, S. L., Muster, N., Burckin, T. A., Wachter, K. C., Emigh, C. A., McCleery, J. A., Yates, J. R. III, Hartzog, G. A. (2003). Dual Roles for Spt5 in Pre-mRNA Processing and Transcription Elongation Revealed by Identification of Spt5-Associated Proteins. Mol. Cell. Biol. 23: 1368-1378 [Abstract] [Full Text]
Surabhi, R. M., Gaynor, R. B. (2002). RNA Interference Directed against Viral and Cellular Targets Inhibits Human Immunodeficiency Virus Type 1 Replication. J. Virol. 76: 12963-12973 [Abstract] [Full Text]
Reid, J., Murray, I., Watt, K., Betney, R., McEwan, I. J. (2002). The Androgen Receptor Interacts with Multiple Regions of the Large Subunit of General Transcription Factor TFIIF. J. Biol. Chem. 277: 41247-41253 [Abstract] [Full Text]
Shim, E. Y., Walker, A. K., Shi, Y., Blackwell, T. K. (2002). CDK-9/cyclin T (P-TEFb) is required in two postinitiation pathways for transcription in the C. elegans embryo. Genes Dev. 16: 2135-2146 [Abstract] [Full Text]
Yamaguchi, Y., Inukai, N., Narita, T., Wada, T., Handa, H. (2002). Evidence that Negative Elongation Factor Represses Transcription Elongation through Binding to a DRB Sensitivity-Inducing Factor/RNA Polymerase II Complex and RNA. Mol. Cell. Biol. 22: 2918-2927 [Abstract] [Full Text]
Hoque, M., Young, T. M., Lee, C.-G., Serrero, G., Mathews, M. B., Pe'ery, T. (2002). The Growth Factor Granulin Interacts with Cyclin T1 and Modulates P-TEFb-Dependent Transcription. Mol. Cell. Biol. 23: 1688-1702 [Abstract] [Full Text]
Taube, R., Lin, X., Irwin, D., Fujinaga, K., Peterlin, B. M. (2002). Interaction between P-TEFb and the C-Terminal Domain of RNA Polymerase II Activates Transcriptional Elongation from Sites Upstream or Downstream of Target Genes. Mol. Cell. Biol. 22: 321-331 [Abstract] [Full Text]
Kaplan, C. D., Morris, J. R., Wu, C.-t., Winston, F. (2000). Spt5 and Spt6 are associated with active transcription and have characteristics of general elongation factors in D. melanogaster. Genes Dev. 14: 2623-2634 [Abstract] [Full Text]
Fong, Y. W., Zhou, Q. (2000). Relief of Two Built-In Autoinhibitory Mechanisms in P-TEFb Is Required for Assembly of a Multicomponent Transcription Elongation Complex at the Human Immunodeficiency Virus Type 1 Promoter. Mol. Cell. Biol. 20: 5897-5907 [Abstract] [Full Text]
Ivanov, D., Kwak, Y. T., Guo, J., Gaynor, R. B. (2000). Domains in the SPT5 Protein That Modulate Its Transcriptional Regulatory Properties. Mol. Cell. Biol. 20: 2970-2983 [Abstract] [Full Text]
O'Keeffe, B., Fong, Y., Chen, D., Zhou, S., Zhou, Q. (2000). Requirement for a Kinase-specific Chaperone Pathway in the Production of a Cdk9/Cyclin T1 Heterodimer Responsible for P-TEFb-mediated Tat Stimulation of HIV-1 Transcription. J. Biol. Chem. 275: 279-287 [Abstract] [Full Text]
Ping, Y.-H., Rana, T. M. (2001). DSIF and NELF Interact with RNA Polymerase II Elongation Complex and HIV-1 Tat Stimulates P-TEFb-mediated Phosphorylation of RNA Polymerase II and DSIF during Transcription Elongation. J. Biol. Chem. 276: 12951-12958 [Abstract] [Full Text]
Chiu, Y.-L., Coronel, E., Ho, C. K., Shuman, S., Rana, T. M. (2001). HIV-1 Tat Protein Interacts with Mammalian Capping Enzyme and Stimulates Capping of TAR RNA. J. Biol. Chem. 276: 12959-12966 [Abstract] [Full Text]
Wei, W., Dorjsuren, D., Lin, Y., Qin, W., Nomura, T., Hayashi, N., Murakami, S. (2001). Direct Interaction between the Subunit RAP30 of Transcription Factor IIF (TFIIF) and RNA Polymerase Subunit 5, Which Contributes to the Association between TFIIF and RNA Polymerase II. J. Biol. Chem. 276: 12266-12273 [Abstract] [Full Text]
Kim, J. B., Sharp, P. A. (2001). Positive Transcription Elongation Factor b Phosphorylates hSPT5 and RNA Polymerase II Carboxyl-terminal Domain Independently of Cyclin-dependent Kinase-activating Kinase. J. Biol. Chem. 276: 12317-12323 [Abstract] [Full Text]

J. Bacteriol.	J. Virol.	Eukaryot. Cell

Microbiol. Mol. Biol. Rev.	Clin. Vaccine Immunol.	All ASM Journals