This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, J. B.
Right arrow Articles by Sharp, P. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, J. B.
Right arrow Articles by Sharp, P. A.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, September 1999, p. 5960-5968, Vol. 19, No. 9
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Tat-SF1 Protein Associates with RAP30 and Human SPT5 Proteins

Jae B. Kim,1 Yuki Yamaguchi,2 Tadashi Wada,2 Hiroshi Handa,2 and Phillip A. Sharp1,*

Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139,1 and Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Midori-Ku, Yokohama 226-8501, Japan2

Received 5 April 1999/Returned for modification 17 May 1999/Accepted 9 June 1999

The potent transactivator Tat recognizes the transactivation response RNA element (TAR) of human immunodeficiency virus type 1 and stimulates the processivity of elongation of RNA polymerase (Pol) II complexes. The cellular proteins Tat-SF1 and human SPT5 (hSPT5) are required for Tat activation as shown by immunodepletion with specific sera and complementation with recombinant proteins. In nuclear extracts, small fractions of both hSPT5 and Pol II are associated with Tat-SF1 protein. Surprisingly, the RAP30 protein of the heterodimeric transcription TFIIF factor is associated with Tat-SF1, while the RAP74 subunit of TFIIF is not coimmunoprecipitated with Tat-SF1. Overexpression of Tat-SF1 and hSPT5 specifically stimulates the transcriptional activity of Tat in vivo. These results suggest that Tat-SF1 and hSPT5 are indispensable cellular factors supporting Tat-specific transcription activation and that they may interact with RAP30 in controlling elongation.

* Corresponding author. Mailing address: Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA 02139. Phone: (617) 253-6421. Fax: (617) 253-3867. E-mail: sharppa{at}mit.edu.

Molecular and Cellular Biology, September 1999, p. 5960-5968, Vol. 19, No. 9
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Zhou, Q., Yik, J. H. N. (2006). The Yin and Yang of P-TEFb Regulation: Implications for Human Immunodeficiency Virus Gene Expression and Global Control of Cell Growth and Differentiation. Microbiol. Mol. Biol. Rev. 70: 646-659 [Abstract] [Full Text]  
  • Wu, C.-H., Yamaguchi, Y., Benjamin, L. R., Horvat-Gordon, M., Washinsky, J., Enerly, E., Larsson, J., Lambertsson, A., Handa, H., Gilmour, D. (2003). NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila. Genes Dev. 17: 1402-1414 [Abstract] [Full Text]  
  • Narita, T., Yamaguchi, Y., Yano, K., Sugimoto, S., Chanarat, S., Wada, T., Kim, D.-k., Hasegawa, J., Omori, M., Inukai, N., Endoh, M., Yamada, T., Handa, H. (2003). Human Transcription Elongation Factor NELF: Identification of Novel Subunits and Reconstitution of the Functionally Active Complex. Mol. Cell. Biol. 23: 1863-1873 [Abstract] [Full Text]  
  • Lindstrom, D. L., Squazzo, S. L., Muster, N., Burckin, T. A., Wachter, K. C., Emigh, C. A., McCleery, J. A., Yates, J. R. III, Hartzog, G. A. (2003). Dual Roles for Spt5 in Pre-mRNA Processing and Transcription Elongation Revealed by Identification of Spt5-Associated Proteins. Mol. Cell. Biol. 23: 1368-1378 [Abstract] [Full Text]  
  • Surabhi, R. M., Gaynor, R. B. (2002). RNA Interference Directed against Viral and Cellular Targets Inhibits Human Immunodeficiency Virus Type 1 Replication. J. Virol. 76: 12963-12973 [Abstract] [Full Text]  
  • Reid, J., Murray, I., Watt, K., Betney, R., McEwan, I. J. (2002). The Androgen Receptor Interacts with Multiple Regions of the Large Subunit of General Transcription Factor TFIIF. J. Biol. Chem. 277: 41247-41253 [Abstract] [Full Text]  
  • Shim, E. Y., Walker, A. K., Shi, Y., Blackwell, T. K. (2002). CDK-9/cyclin T (P-TEFb) is required in two postinitiation pathways for transcription in the C. elegans embryo. Genes Dev. 16: 2135-2146 [Abstract] [Full Text]  
  • Yamaguchi, Y., Inukai, N., Narita, T., Wada, T., Handa, H. (2002). Evidence that Negative Elongation Factor Represses Transcription Elongation through Binding to a DRB Sensitivity-Inducing Factor/RNA Polymerase II Complex and RNA. Mol. Cell. Biol. 22: 2918-2927 [Abstract] [Full Text]  
  • Hoque, M., Young, T. M., Lee, C.-G., Serrero, G., Mathews, M. B., Pe'ery, T. (2002). The Growth Factor Granulin Interacts with Cyclin T1 and Modulates P-TEFb-Dependent Transcription. Mol. Cell. Biol. 23: 1688-1702 [Abstract] [Full Text]  
  • Taube, R., Lin, X., Irwin, D., Fujinaga, K., Peterlin, B. M. (2002). Interaction between P-TEFb and the C-Terminal Domain of RNA Polymerase II Activates Transcriptional Elongation from Sites Upstream or Downstream of Target Genes. Mol. Cell. Biol. 22: 321-331 [Abstract] [Full Text]  
  • Kaplan, C. D., Morris, J. R., Wu, C.-t., Winston, F. (2000). Spt5 and Spt6 are associated with active transcription and have characteristics of general elongation factors in D. melanogaster. Genes Dev. 14: 2623-2634 [Abstract] [Full Text]  
  • Fong, Y. W., Zhou, Q. (2000). Relief of Two Built-In Autoinhibitory Mechanisms in P-TEFb Is Required for Assembly of a Multicomponent Transcription Elongation Complex at the Human Immunodeficiency Virus Type 1 Promoter. Mol. Cell. Biol. 20: 5897-5907 [Abstract] [Full Text]  
  • Ivanov, D., Kwak, Y. T., Guo, J., Gaynor, R. B. (2000). Domains in the SPT5 Protein That Modulate Its Transcriptional Regulatory Properties. Mol. Cell. Biol. 20: 2970-2983 [Abstract] [Full Text]  
  • O'Keeffe, B., Fong, Y., Chen, D., Zhou, S., Zhou, Q. (2000). Requirement for a Kinase-specific Chaperone Pathway in the Production of a Cdk9/Cyclin T1 Heterodimer Responsible for P-TEFb-mediated Tat Stimulation of HIV-1 Transcription. J. Biol. Chem. 275: 279-287 [Abstract] [Full Text]  
  • Ping, Y.-H., Rana, T. M. (2001). DSIF and NELF Interact with RNA Polymerase II Elongation Complex and HIV-1 Tat Stimulates P-TEFb-mediated Phosphorylation of RNA Polymerase II and DSIF during Transcription Elongation. J. Biol. Chem. 276: 12951-12958 [Abstract] [Full Text]  
  • Chiu, Y.-L., Coronel, E., Ho, C. K., Shuman, S., Rana, T. M. (2001). HIV-1 Tat Protein Interacts with Mammalian Capping Enzyme and Stimulates Capping of TAR RNA. J. Biol. Chem. 276: 12959-12966 [Abstract] [Full Text]  
  • Wei, W., Dorjsuren, D., Lin, Y., Qin, W., Nomura, T., Hayashi, N., Murakami, S. (2001). Direct Interaction between the Subunit RAP30 of Transcription Factor IIF (TFIIF) and RNA Polymerase Subunit 5, Which Contributes to the Association between TFIIF and RNA Polymerase II. J. Biol. Chem. 276: 12266-12273 [Abstract] [Full Text]  
  • Kim, J. B., Sharp, P. A. (2001). Positive Transcription Elongation Factor b Phosphorylates hSPT5 and RNA Polymerase II Carboxyl-terminal Domain Independently of Cyclin-dependent Kinase-activating Kinase. J. Biol. Chem. 276: 12317-12323 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»