MCM Proteins Are Associated with RNA Polymerase II Holoenzyme

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Yankulov, K.
	Articles by Bentley, D. L.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Yankulov, K.
	Articles by Bentley, D. L.

Molecular and Cellular Biology, September 1999, p. 6154-6163, Vol. 19, No. 9
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

MCM Proteins Are Associated with RNA Polymerase II Holoenzyme

K. Yankulov,¹^,^* I. Todorov,² P. Romanowski,³ D. Licatalosi,⁴ K. Cilli,⁴ S. McCracken,⁵ R. Laskey,³ and D. L. Bentley⁴

Department of Molecular Biology and Genetics, University of Guelph, Guelph, Ontario N1G 2W1,¹ and Banting & Best Department of Medical Research, University of Toronto, Toronto, Ontario M5G 1L6,⁵ Canada; Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver, Colorado 80262⁴; Desmos Inc., San Diego, California 92121²; and Wellcome/CRC Institute, Cambridge CB2 1OR, United Kingdom³

Received 20 April 1999/Returned for modification 26 May 1999/Accepted 1 June 1999

MCMs are a family of proteins related to ATP-dependent helicases that bind to origin recognition complexes and are requiredfor initiation of DNA replication. We report that antibodies againstMCM2(BM28) specifically inhibited transcription by RNA polymeraseII (Pol II) in microinjected Xenopus oocytes. Consistent withthis observation, MCM2 and other MCMs copurified with Pol II andgeneral transcription factors (GTFs) in high-molecular-weightholoenzyme complexes isolated from Xenopus oocytes and HeLa cells.Pol II and GTFs also copurified with MCMs isolated by anti-MCM3immunoaffinity chromatography. MCMs were specifically displacedfrom the holoenzyme complex by antibody against the C-terminaldomain (CTD) of Pol II. In addition, MCMs bound to a CTD affinitycolumn, suggesting that their association with holoenzyme dependsin part on this domain of Pol II. These results suggest a newfunction for MCM proteins as components of the Pol II transcriptionalapparatus.

^* Corresponding author. Mailing address: Department of Molecular Biology and Genetics, University of Guelph, Guelph, OntarioN1G 2W1, Canada. Phone: (519) 824-4120, ext. 6466. Fax: (519)837-2075. E-mail: yankulov{at}uoguelph.ca.

Molecular and Cellular Biology, September 1999, p. 6154-6163, Vol. 19, No. 9
0270-7306/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

Ge, X. Q., Jackson, D. A., Blow, J. J. (2007). Dormant origins licensed by excess Mcm2 7 are required for human cells to survive replicative stress. Genes Dev. 21: 3331-3341 [Abstract] [Full Text]
Lake, C. M., Teeter, K., Page, S. L., Nielsen, R., Hawley, R. S. (2007). A Genetic Analysis of the Drosophila mcm5 Gene Defines a Domain Specifically Required for Meiotic Recombination. Genetics 176: 2151-2163 [Abstract] [Full Text]
Shin, J.-H., Santangelo, T. J., Xie, Y., Reeve, J. N., Kelman, Z. (2007). Archaeal Minichromosome Maintenance (MCM) Helicase Can Unwind DNA Bound by Archaeal Histones and Transcription Factors. J. Biol. Chem. 282: 4908-4915 [Abstract] [Full Text]
Shin, J.-H., Kelman, Z. (2006). The Replicative Helicases of Bacteria, Archaea, and Eukarya Can Unwind RNA-DNA Hybrid Substrates. J. Biol. Chem. 281: 26914-26921 [Abstract] [Full Text]
Gorisch, S. M., Wachsmuth, M., Toth, K. F., Lichter, P., Rippe, K. (2005). Histone acetylation increases chromatin accessibility. J. Cell Sci. 118: 5825-5834 [Abstract] [Full Text]
Snyder, M., He, W., Zhang, J. J. (2005). The DNA replication factor MCM5 is essential for Stat1-mediated transcriptional activation. Proc. Natl. Acad. Sci. USA 102: 14539-14544 [Abstract] [Full Text]
Shechter, D., Gautier, J. (2004). MCM proteins and checkpoint kinases get together at the fork. Proc. Natl. Acad. Sci. USA 101: 10845-10846 [Full Text]
Forsburg, S. L. (2004). Eukaryotic MCM Proteins: Beyond Replication Initiation. Microbiol. Mol. Biol. Rev. 68: 109-131 [Abstract] [Full Text]
Kim, S. J., Martinson, H. G. (2003). Poly(A)-dependent Transcription Termination: CONTINUED COMMUNICATION OF THE POLY(A) SIGNAL WITH THE POLYMERASE IS REQUIRED LONG AFTER EXTRUSION IN VIVO. J. Biol. Chem. 278: 41691-41701 [Abstract] [Full Text]
Dziak, R., Leishman, D., Radovic, M., Tye, B. K., Yankulov, K. (2003). Evidence for a Role of MCM (Mini-chromosome Maintenance)5 in Transcriptional Repression of Sub-telomeric and Ty-proximal Genes in Saccharomyces cerevisiae. J. Biol. Chem. 278: 27372-27381 [Abstract] [Full Text]
Fitch, M. J., Donato, J. J., Tye, B. K. (2003). Mcm7, a Subunit of the Presumptive MCM Helicase, Modulates Its Own Expression in Conjunction with Mcm1. J. Biol. Chem. 278: 25408-25416 [Abstract] [Full Text]
Bukholm, I R K, Bukholm, G, Holm, R, Nesland, J M (2003). Association between histology grade, expression of HsMCM2, and cyclin A in human invasive breast carcinomas. J. Clin. Pathol. 56: 368-373 [Abstract] [Full Text]
Gozuacik, D., Chami, M., Lagorce, D., Faivre, J., Murakami, Y., Poch, O., Biermann, E., Knippers, R., Brechot, C., Paterlini-Brechot, P. (2003). Identification and functional characterization of a new member of the human Mcm protein family: hMcm8. Nucleic Acids Res 31: 570-579 [Abstract] [Full Text]
Gauthier, L., Dziak, R., Kramer, D. J. H., Leishman, D., Song, X., Ho, J., Radovic, M., Bentley, D., Yankulov, K. (2002). The Role of the Carboxyterminal Domain of RNA Polymerase II in Regulating Origins of DNA Replication in Saccharomyces cerevisiae. Genetics 162: 1117-1129 [Abstract] [Full Text]
Schaarschmidt, D., Ladenburger, E.-M., Keller, C., Knippers, R. (2002). Human Mcm proteins at a replication origin during the G1 to S phase transition. Nucleic Acids Res 30: 4176-4185 [Abstract] [Full Text]
Shohet, J. M., Hicks, M. J., Plon, S. E., Burlingame, S. M., Stuart, S., Chen, S.-Y., Brenner, M. K., Nuchtern, J. G. (2002). Minichromosome Maintenance Protein MCM7 Is a Direct Target of the MYCN Transcription Factor in Neuroblastoma. Cancer Res. 62: 1123-1128 [Abstract] [Full Text]
Dimitrova, D. S., Prokhorova, T. A., Blow, J. J., Todorov, I. T., Gilbert, D. M. (2002). Mammalian nuclei become licensed for DNA replication during late telophase. J. Cell Sci. 115: 51-59 [Abstract] [Full Text]
Ishimi, Y., Komamura-Kohno, Y., Arai, K.-i., Masai, H. (2001). Biochemical Activities Associated with Mouse Mcm2 Protein. J. Biol. Chem. 276: 42744-42752 [Abstract] [Full Text]
Jenkins, H. L., Spencer, C. A. (2001). RNA Polymerase II Holoenzyme Modifications Accompany Transcription Reprogramming in Herpes Simplex Virus Type 1-Infected Cells. J. Virol. 75: 9872-9884 [Abstract] [Full Text]
Fong, N., Bentley, D. L. (2001). Capping, splicing, and 3' processing are independently stimulated by RNA polymerase II: different functions for different segments of the CTD. Genes Dev. 15: 1783-1795 [Abstract] [Full Text]
DaFonseca, C. J., Shu, F., Zhang, J. J. (2001). Identification of two residues in MCM5 critical for the assembly of minichromosome maintenance complexes and signal transducer and activator of transcription-mediated transcription activation in response to IFN-gamma. Proc. Natl. Acad. Sci. USA 10.1073/pnas.061487598v1 [Abstract] [Full Text]
Szentirmay, M. N., Sawadogo, M. (2000). SURVEY AND SUMMARY: Spatial organization of RNA polymerase II transcription in the nucleus. Nucleic Acids Res 28: 2019-2025 [Abstract] [Full Text]
Lim, C. R., Kimata, Y., Ohdate, H., Kokubo, T., Kikuchi, N., Horigome, T., Kohno, K. (2000). The Saccharomyces cerevisiae RuvB-like Protein, Tih2p, Is Required for Cell Cycle Progression and RNA Polymerase II-directed Transcription. J. Biol. Chem. 275: 22409-22417 [Abstract] [Full Text]
Alexandrow, M. G., Ritzi, M., Pemov, A., Hamlin, J. L. (2002). A Potential Role for Mini-chromosome Maintenance (MCM) Proteins in Initiation at the Dihydrofolate Reductase Replication Origin. J. Biol. Chem. 277: 2702-2708 [Abstract] [Full Text]
Tye, B. K., Sawyer, S. (2000). The Hexameric Eukaryotic MCM Helicase: Building Symmetry from Nonidentical Parts. J. Biol. Chem. 275: 34833-34836 [Full Text]
DaFonseca, C. J., Shu, F., Zhang, J. J. (2001). Identification of two residues in MCM5 critical for the assembly of MCM complexes and Stat1-mediated transcription activation in response to IFN-gamma. Proc. Natl. Acad. Sci. USA 98: 3034-3039 [Abstract] [Full Text]

J. Bacteriol.	J. Virol.	Eukaryot. Cell

Microbiol. Mol. Biol. Rev.	Clin. Vaccine Immunol.	All ASM Journals