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Mol Cell Biol. 1982 November; 2(11): 1459-1462
Copyright © 1982, American Society for Microbiology. All Rights Reserved.

Alterations of Gene Structure in Ethyl Methane Sulfonate-Induced Mutants of Mammalian Cells

¹ Laboratory of Molecular Genetics, Clinical Research Institute of Montreal, Montreal, Quebec H2W 1R7, Canada
² Department of Biochemistry, St. Mary's Hospital Medical School, London W2 1PG, England

ABSTRACT

To determine the types of alterations in gene structure induced by DNA-alkylating agents, we analyzed the restriction enzyme cleavage patterns of adenine phosphoribosyltransferase gene sequences in mutant strains of Chinese hamster ovary cells deficient in this enzyme. Base pair changes as detected by loss of restriction enzyme sites were found, but no major internal gene rearrangements could be detected.