This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kurjan, J. Articles by Hall, B. D. Search for Related Content PubMed PubMed Citation Articles by Kurjan, J. Articles by Hall, B. D.

 Previous Article  |  Next Article 

Mol Cell Biol. 1982 December; 2(12): 1501-1513
Copyright © 1982, American Society for Microbiology. All Rights Reserved.

Mutations at the Saccharomyces cerevisiae SUP4 tRNA^Tyr Locus: Isolation, Genetic Fine-Structure Mapping, and Correlation with Physical Structure

Janet Kurjan and Benjamin D. Hall

¹ Department of Genetics, University of Washington, Seattle, Washington 98195

ABSTRACT

The SUP4 tRNA^Tyr locus in Saccharomyces cerevisiae has been studied by the isolation and characterization of mutations at the SUP4 gene which result in the loss of suppressor function. Most of the mutations act as single-site mutations, whereas about a third of the mutations are deletions of the entire gene. Two meiotic fine-structure maps of the gene were made. The first mapping technique placed 10 mutations plus the sup4⁺ anticodon on a map by a measurement of levels of recombination between pairs of mutations. The second map utilized a more qualitative estimate of recombination frequency, allowing 69 mutations and the sup4⁺ anticodon to be mapped. The maps were compared with the physical structure of the gene for the 34 mutations whose nucleotide alteration has been determined by DNA sequencing (Koski et al., Cell 22:415-425, 1980; Kurjan et al., Cell 20:701-709, 1980). Both maps show a good correlation with the physical structure of the gene, even though certain properties of genetic fine-structure maps, such as marker effects and "map expansion," were seen.

Present address: Department of Biological Sciences, Columbia University, New York, NY 10027.

This article has been cited by other articles:

• Bobkova, E. V., Hall, B. D. (1997). Substrate Specificity of the RNase Activity of Yeast RNA Polymerase III. J. Biol. Chem. 272: 22832-22839 [Abstract] [Full Text]  
• Chalker, D L, Sandmeyer, S B (1992). Ty3 integrates within the region of RNA polymerase III transcription initiation.. Genes Dev. 6: 117-128 [Abstract]