This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Morton, D. G. Articles by Sprague, K. U. Search for Related Content PubMed PubMed Citation Articles by Morton, D. G. Articles by Sprague, K. U.

 Previous Article  |  Next Article 

Mol Cell Biol. 1982 December; 2(12): 1524-1531
Copyright © 1982, American Society for Microbiology. All Rights Reserved.

Silkworm 5S RNA and Alanine tRNA Genes Share Highly Conserved 5' Flanking and Coding Sequences

Institute of Molecular Biology and Biology Department, University of Oregon, Eugene, Oregon 97403

ABSTRACT

A fragment of Bombyx mori genomic DNA containing one tRNA₂^Ala gene and one 5S RNA gene has been used to compare the structural features of silkworm 5S RNA and tRNA genes. The nucleotide sequences of both genes and of the primary transcripts produced from them in homologous in vitro transcription systems have been determined. Comparison of the sequences of these two genes with that of another previously analyzed B. mori tRNA₂^Ala gene reveals common oligonucleotides which may be important transcriptional signals. The oligonucleotides TA(C)TAT, AATTTT, and TTC are located approximately (±1 nucleotide) 29, 19, and 3 nucleotides, respectively, before the transcription initiation sites of the two tRNA₂^Ala genes and the one 5S RNA gene we have analyzed. The sequence GGGCGTAG(C)TCAG lies within the coding regions of all three genes. The functional significance of these sequences is suggested by their location within regions required for the transcription of silkworm alanine tRNA genes in vitro.

This article has been cited by other articles:

• Young, L., Dunstan, H., Witte, P., Smith, T., Ottonello, S, Sprague, K. (1991). A class III transcription factor composed of RNA. Science 252: 542-546 [Abstract]