This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by An, G.
Right arrow Articles by Siminovitch, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by An, G.
Right arrow Articles by Siminovitch, L.

 Previous Article  |  Next Article 

Mol Cell Biol. 1982 December; 2(12): 1628-1632
Copyright © 1982, American Society for Microbiology. All Rights Reserved.

Expression of Bacterial ß-Galactosidase in Animal Cells

Gynheung An{dagger}, Katsuhiko Hidaka{ddagger} and Louis Siminovitch

1 Department of Medical Genetics, University of Toronto, and The Hospital for Sick Children, Toronto, Ontario, Canada, M5G 1X8

ABSTRACT

A recombinant plasmid containing the gene for bacterial ß-galactosidase, situated close to the simian virus 40 early promoter, has been constructed. Transfection of CHO, L, and COS-1 cells with this plasmid led to the expression and appearance of the enzyme. Using this system, we have developed a series of promoter cloning vehicles capable of accepting promoter signals for animal genes.

FOOTNOTES

{dagger} Present address: Department of Microbiology and Immunology, University of Washington, Seattle, WA 98195.

{ddagger} Present address: Department of Surgery, Saga-Medical School, Nabeshima-Machi, Saga-City 840-01, Japan.

Mol Cell Biol. 1982 December; 2(12): 1628-1632
Copyright © 1982, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Bolon, B. (2008). Whole Mount Enzyme Histochemistry as a Rapid Screen at Necropsy for Expression of {beta}-Galactosidase (LacZ)-Bearing Transgenes: Considerations for Separating Specific LacZ Activity from Nonspecific (Endogenous) Galactosidase Activity. Toxicol Pathol 36: 265-276 [Abstract] [Full Text]  
  • Cote-Velez, A, Perez-Martinez, L, Diaz-Gallardo, M Y, Perez-Monter, C, Carreon-Rodriguez, A, Charli, J-L, Joseph-Bravo, P (2005). Dexamethasone represses cAMP rapid upregulation of TRH gene transcription: identification of a composite glucocorticoid response element and a cAMP response element in TRH promoter. J Mol Endocrinol 34: 177-197 [Abstract] [Full Text]  
  • Piechocki, M. P., Burk, R. D., Ruch, R. J. (1999). Regulation of connexin32 and connexin43 gene expression by DNA methylation in rat liver cells. Carcinogenesis 20: 401-406 [Abstract] [Full Text]  
  • Zetser, A., Gredinger, E., Bengal, E. (1999). p38 Mitogen-activated Protein Kinase Pathway Promotes Skeletal Muscle Differentiation. PARTICIPATION OF THE MEF2C TRANSCRIPTION FACTOR. J. Biol. Chem. 274: 5193-5200 [Abstract] [Full Text]  
  • Ishiguro, H., Yamada, K., Ichino, N., Nagatsu, T. (1998). Identification and Characterization of a Novel Phorbol Ester-responsive DNA Sequence in the 5'-Flanking Region of the Human Dopamine beta -Hydroxylase Gene. J. Biol. Chem. 273: 21941-21949 [Abstract] [Full Text]  
  • Gredinger, E., Gerber, A. N., Tamir, Y., Tapscott, S. J., Bengal, E. (1998). Mitogen-activated Protein Kinase Pathway Is Involved in the Differentiation of Muscle Cells. J. Biol. Chem. 273: 10436-10444 [Abstract] [Full Text]  
  • Kumar, A. S., Venkatesh, V. C., Planer, B. C., Feinstein, S. I., Ballard, P. L. (1997). Phorbol Ester Down-regulation of Lung Surfactant Protein B Gene Expression by Cytoplasmic Trapping of Thyroid Transcription Factor-1 and Hepatocyte Nuclear Factor 3. J. Biol. Chem. 272: 20764-20773 [Abstract] [Full Text]  
  • Tinti, C., Yang, C., Seo, H., Conti, B., Kim, C., Joh, T. H., Kim, K.-S. (1997). Structure/Function Relationship of the cAMP Response Element in Tyrosine Hydroxylase Gene Transcription. J. Biol. Chem. 272: 19158-19164 [Abstract] [Full Text]  
  • Schuur, E. R., Henderson, G. A., Kmetec, L. A., Miller, J. D., Lamparski, H. G., Henderson, D. R. (1996). Prostate-specific Antigen Expression Is Regulated by an Upstream Enhancer. J. Biol. Chem. 271: 7043-7051 [Abstract] [Full Text]  
  • Chang, L., Thompson, M. A. (1996). Activity of the Distal Positive Element of the Peripherin Gene Is Dependent on Proteins Binding to an Ets-like Recognition Site and a Novel Inverted Repeat Site. J. Biol. Chem. 271: 6467-6475 [Abstract] [Full Text]  
  • Yao, H., Labudda, K., Rim, C., Capodieci, P., Loda, M., Stork, P. J. S. (1995). Cyclic Adenosine Monophosphate Can Convert Epidermal Growth Factor into a Differentiating Factor in Neuronal Cells. J. Biol. Chem. 270: 20748-20753 [Abstract] [Full Text]  
  • Carroll, J. M., Taichman, L. B. (1992). Characterization of the human involucrin promoter using a transient beta-galactosidase assay. J. Cell Sci. 103: 925-930 [Abstract]  
  • Edlund, T, Walker, M., Barr, P., Rutter, W. (1985). Cell-specific expression of the rat insulin gene: evidence for role of two distinct 5' flanking elements. Science 230: 912-916 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»