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Expression of Bacterial ß-Galactosidase in Animal Cells

Gynheung An, Katsuhiko Hidaka and Louis Siminovitch

¹ Department of Medical Genetics, University of Toronto, and The Hospital for Sick Children, Toronto, Ontario, Canada, M5G 1X8

ABSTRACT

A recombinant plasmid containing the gene for bacterial ß-galactosidase, situated close to the simian virus 40 early promoter, has been constructed. Transfection of CHO, L, and COS-1 cells with this plasmid led to the expression and appearance of the enzyme. Using this system, we have developed a series of promoter cloning vehicles capable of accepting promoter signals for animal genes.

Present address: Department of Microbiology and Immunology, University of Washington, Seattle, WA 98195.

Present address: Department of Surgery, Saga-Medical School, Nabeshima-Machi, Saga-City 840-01, Japan.

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