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Localization of Cap-Binding Protein in Subcellular Fractions of HeLa Cells

Joanna L. Hansen^1,, Diane O. Etchison², John W. B. Hershey² and Ellie Ehrenfeld^1,3

¹ Department of Cellular, Viral and Molecular Biology, University of Utah Medical Center, Salt Lake City, Utah 84132
³ Department of Biochemistry, University of Utah Medical Center, Salt Lake City, Utah 84132
² Department of Biological Chemistry, School of Medicine, University of California, Davis, California 95616

ABSTRACT

The 26,000-M_r cap-binding protein was analyzed by a cross-linking assay in cell fractions from uninfected and poliovirus-infected HeLa cells. Cap-binding protein was found in the postribosomal supernatant (S-200) and in the ribosomal salt wash. The cap-binding protein in the S-200 had a sedimentation coefficient of 5 to 7S and lacked the ability to restore translation in extracts of poliovirus-infected cells.

Present address: Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20205.

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