Previous Article | Next Article 
Mol Cell Biol. 1982 June; 2(6): 617-624
Transcripts from the Cellular Homologs of Retroviral Oncogenes: Distribution Among Chicken Tissues
Thomas J. Gonda,
Diana K. Sheiness and
J. Michael Bishop
1 Department of Microbiology and Immunology, University of California, San Francisco, California 94143
ABSTRACT
The oncogenes (v-onc genes) of rapidly transforming retroviruses have homologs (c-onc genes) in the genomes of normal cells. In this study, we characterized and quantitated transcription from four c-onc genes, c-myb, c-myc, c-erb, and c-src, in a variety of chicken cells and tissues. Electrophoretic analysis of polyadenylated RNA, followed by transfer to nitrocellulose and hybridization to cloned onc probes showed that c-myb, c-myc, and c-src each give rise to a single mature transcript, whereas c-erb gives rise to multiple transcripts (B. Vennstrom and J. M. Bishop, Cell, in press) which vary in abundance among different cells and tissues. Transcription from c-myb, c-myc, c-erb, and c-src was quantitated by a "dot-blot" hybridization assay. We found that c-myc, c-erb, and c-src transcription could be detected in nearly all cells and tissues examined, whereas c-myb transcription was detected only in some hemopoietic cells; these cells, however, belong to several different lineages. Thus, in no case was expression of a c-onc gene restricted to a single cell lineage. There appeared to be a correlation between levels of c-myb expression and hemopoietic activity of the tissues and cells examined, which suggests that c-myb may be expressed primarily in immature hemopoietic cells. An examination of c-onc RNA levels in target cells and tissues for viruses carrying the corresponding v-onc genes revealed no obvious correlation, direct or inverse, between susceptibility to transformation by a given v-onc gene and expression of the homologous c-onc gene.
Mol Cell Biol. 1982 June; 2(6): 617-624
This article has been cited by other articles:
-
Drabsch, Y., Hugo, H., Zhang, R., Dowhan, D. H., Miao, Y. R., Gewirtz, A. M., Barry, S. C., Ramsay, R. G., Gonda, T. J.
(2007). Mechanism of and requirement for estrogen-regulated MYB expression in estrogen-receptor-positive breast cancer cells. Proc. Natl. Acad. Sci. USA
104: 13762-13767
[Abstract]
[Full Text]
-
Shor, A. C., Keschman, E. A., Lee, F. Y., Muro-Cacho, C., Letson, G. D., Trent, J. C., Pledger, W. J., Jove, R.
(2007). Dasatinib Inhibits Migration and Invasion in Diverse Human Sarcoma Cell Lines and Induces Apoptosis in Bone Sarcoma Cells Dependent on Src Kinase for Survival. Cancer Res.
67: 2800-2808
[Abstract]
[Full Text]
-
Sinclair, A. M., Bench, A. J., Bloor, A. J. C., Li, J., Gottgens, B., Stanley, M. L., Miller, J., Piltz, S., Hunter, S., Nacheva, E. P., Sanchez, M.-J., Green, A. R.
(2002). Rescue of the lethal scl-/- phenotype by the human SCL locus. Blood
99: 3931-3938
[Abstract]
[Full Text]
-
Hardy, M. P., Owczarek, C. M., Trajanovska, S., Liu, X., Kola, I., Hertzog, P. J.
(2001). The soluble murine type I interferon receptor Ifnar-2 is present in serum, is independently regulated, and has both agonistic and antagonistic properties. Blood
97: 473-482
[Abstract]
[Full Text]
-
Tavner, F. J., Simpson, R., Tashiro, S., Favier, D., Jenkins, N. A., Gilbert, D. J., Copeland, N. G., Macmillan, E. M., Lutwyche, J., Keough, R. A., Ishii, S., Gonda, T. J.
(1998). Molecular Cloning Reveals that the p160 Myb-Binding Protein Is a Novel, Predominantly Nucleolar Protein Which May Play a Role in Transactivation by Myb. Mol. Cell. Biol.
18: 989-1002
[Abstract]
[Full Text]
-
Owczarek, C. M., Hwang, S. Y., Holland, K. A., Gulluyan, L. M., Tavaria, M., Weaver, B., Reich, N. C., Kola, I., Hertzog, P. J.
(1997). Cloning and Characterization of Soluble and Transmembrane Isoforms of a Novel Component of the Murine Type I Interferon Receptor, IFNAR 2. J. Biol. Chem.
272: 23865-23870
[Abstract]
[Full Text]
-
Holland, K. A., Owczarek, C. M., Hwang, S. Y., Tymms, M. J., Constantinescu, S. N., Pfeffer, L. M., Kola, I., Hertzog, P. J.
(1997). A Type I Interferon Signaling Factor, ISF21, Encoded on Chromosome 21 Is Distinct from Receptor Components and Their Down-regulation and Is Necessary for Transcriptional Activation of Interferon-regulated Genes. J. Biol. Chem.
272: 21045-21051
[Abstract]
[Full Text]
-
Katzen, A. L., Bishop, J. M.
(1996). myb provides an essential function during Drosophila development. Proc. Natl. Acad. Sci. USA
93: 13955-13960
[Abstract]
[Full Text]
-
Owczarek, C. M., Layton, M. J., Robb, L. G., Nicola, N. A., Begley, C. G.
(1996). Molecular Basis of the Soluble and Membrane-bound Forms of the Murine Leukemia Inhibitory Factor Receptor alpha-Chain. J. Biol. Chem.
271: 5495-5504
[Abstract]
[Full Text]
-
Dike, L., Ingber, D.
(1996). Integrin-dependent induction of early growth response genes in capillary endothelial cells. J. Cell Sci.
109: 2855-2863
[Abstract]
-
Toth, C. R., Hostutler, R. F., Baldwin, A. S.:Jr., Bender, T. P.
(1995). Members of the Nuclear Factor kappaB Family Transactivate the Murine c-myb Gene. J. Biol. Chem.
270: 7661-7671
[Abstract]
[Full Text]
-
Chow, R., Roux, G., Roghani, M, Palmer, M., Rifkin, D., Moscatelli, D., Lang, R.
(1995). FGF suppresses apoptosis and induces differentiation of fibre cells in the mouse lens. Development
121: 4383-4393
[Abstract]
-
Ness, S A, Kowenz-Leutz, E, Casini, T, Graf, T, Leutz, A
(1993). Myb and NF-M: combinatorial activators of myeloid genes in heterologous cell types.. Genes Dev.
7: 749-759
[Abstract]
-
Leonard, M., Lim, K., Engel, J.
(1993). Expression of the chicken GATA factor family during early erythroid development and differentiation. Development
119: 519-531
[Abstract]
-
Murphy, M, Reid, K, Brown, M., Bartlett, P.
(1993). Involvement of leukemia inhibitory factor and nerve growth factor in the development of dorsal root ganglion neurons. Development
117: 1173-1182
[Abstract]
-
Luscher, B, Eisenman, R N
(1990). New light on Myc and Myb. Part II. Myb.. Genes Dev.
4: 2235-2241
-
Tice-Baldwin, K, Fink, G., Arndt, K.
(1989). BAS1 has a Myb motif and activates HIS4 transcription only in combination with BAS2. Science
246: 931-935
[Abstract]
-
Klempnauer, K H, Arnold, H, Biedenkapp, H
(1989). Activation of transcription by v-myb: evidence for two different mechanisms.. Genes Dev.
3: 1582-1589
[Abstract]
-
Bender, T., Thompson, C., Kuehl, W.
(1987). Differential expression of c-myb mRNA in murine B lymphomas by a block to transcription elongation. Science
237: 1473-1476
[Abstract]
-
Barletta, C, Pelicci, P., Kenyon, L., Smith, S., Dalla-Favera, R
(1987). Relationship between the c-myb locus and the 6q-chromosomal aberration in leukemias and lymphomas. Science
235: 1064-1067
[Abstract]
-
Stern, J., Smith, K.
(1986). Interleukin-2 induction of T-cell G1 progression and c-myb expression. Science
233: 203-206
[Abstract]
-
Bishop, J.M., Drees, B., Katzen, A.L., Kornberg, T.B., Simon, M.A.
(1985). Proto-oncogenes of Drosophila melanogaster. Cold Spring Harb Symp Quant Biol
50: 727-731
[Abstract]
-
Mountz, J., Steinberg, A., Klinman, D., Smith, H., Mushinski, J.
(1984). Autoimmunity and increased c-myb transcription. Science
226: 1087-1089
[Abstract]
-
Stewart, T., Bellve, A., Leder, P
(1984). Transcription and promoter usage of the myc gene in normal somatic and spermatogenic cells. Science
226: 707-710
[Abstract]
-
CLINE, M. J., SLAMON, D. J., LIPSICK, J. S.
(1984). Oncogenes: Implications for the Diagnosis and Treatment of Cancer. ANN INTERN MED
101: 223-233
[Abstract]
-
Slamon, D., deKernion, J., Verma, I., Cline, M.
(1984). Expression of cellular oncogenes in human malignancies. Science
224: 256-262
[Abstract]