This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gonda, T. J.
Right arrow Articles by Bishop, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gonda, T. J.
Right arrow Articles by Bishop, J. M.

 Previous Article  |  Next Article 

Mol Cell Biol. 1982 June; 2(6): 617-624

Transcripts from the Cellular Homologs of Retroviral Oncogenes: Distribution Among Chicken Tissues

Thomas J. Gonda, Diana K. Sheiness and J. Michael Bishop

1 Department of Microbiology and Immunology, University of California, San Francisco, California 94143

ABSTRACT

The oncogenes (v-onc genes) of rapidly transforming retroviruses have homologs (c-onc genes) in the genomes of normal cells. In this study, we characterized and quantitated transcription from four c-onc genes, c-myb, c-myc, c-erb, and c-src, in a variety of chicken cells and tissues. Electrophoretic analysis of polyadenylated RNA, followed by transfer to nitrocellulose and hybridization to cloned onc probes showed that c-myb, c-myc, and c-src each give rise to a single mature transcript, whereas c-erb gives rise to multiple transcripts (B. Vennstrom and J. M. Bishop, Cell, in press) which vary in abundance among different cells and tissues. Transcription from c-myb, c-myc, c-erb, and c-src was quantitated by a "dot-blot" hybridization assay. We found that c-myc, c-erb, and c-src transcription could be detected in nearly all cells and tissues examined, whereas c-myb transcription was detected only in some hemopoietic cells; these cells, however, belong to several different lineages. Thus, in no case was expression of a c-onc gene restricted to a single cell lineage. There appeared to be a correlation between levels of c-myb expression and hemopoietic activity of the tissues and cells examined, which suggests that c-myb may be expressed primarily in immature hemopoietic cells. An examination of c-onc RNA levels in target cells and tissues for viruses carrying the corresponding v-onc genes revealed no obvious correlation, direct or inverse, between susceptibility to transformation by a given v-onc gene and expression of the homologous c-onc gene.

Mol Cell Biol. 1982 June; 2(6): 617-624




This article has been cited by other articles:

  • Drabsch, Y., Hugo, H., Zhang, R., Dowhan, D. H., Miao, Y. R., Gewirtz, A. M., Barry, S. C., Ramsay, R. G., Gonda, T. J. (2007). Mechanism of and requirement for estrogen-regulated MYB expression in estrogen-receptor-positive breast cancer cells. Proc. Natl. Acad. Sci. USA 104: 13762-13767 [Abstract] [Full Text]  
  • Shor, A. C., Keschman, E. A., Lee, F. Y., Muro-Cacho, C., Letson, G. D., Trent, J. C., Pledger, W. J., Jove, R. (2007). Dasatinib Inhibits Migration and Invasion in Diverse Human Sarcoma Cell Lines and Induces Apoptosis in Bone Sarcoma Cells Dependent on Src Kinase for Survival. Cancer Res. 67: 2800-2808 [Abstract] [Full Text]  
  • Sinclair, A. M., Bench, A. J., Bloor, A. J. C., Li, J., Gottgens, B., Stanley, M. L., Miller, J., Piltz, S., Hunter, S., Nacheva, E. P., Sanchez, M.-J., Green, A. R. (2002). Rescue of the lethal scl-/- phenotype by the human SCL locus. Blood 99: 3931-3938 [Abstract] [Full Text]  
  • Hardy, M. P., Owczarek, C. M., Trajanovska, S., Liu, X., Kola, I., Hertzog, P. J. (2001). The soluble murine type I interferon receptor Ifnar-2 is present in serum, is independently regulated, and has both agonistic and antagonistic properties. Blood 97: 473-482 [Abstract] [Full Text]  
  • Tavner, F. J., Simpson, R., Tashiro, S., Favier, D., Jenkins, N. A., Gilbert, D. J., Copeland, N. G., Macmillan, E. M., Lutwyche, J., Keough, R. A., Ishii, S., Gonda, T. J. (1998). Molecular Cloning Reveals that the p160 Myb-Binding Protein Is a Novel, Predominantly Nucleolar Protein Which May Play a Role in Transactivation by Myb. Mol. Cell. Biol. 18: 989-1002 [Abstract] [Full Text]  
  • Owczarek, C. M., Hwang, S. Y., Holland, K. A., Gulluyan, L. M., Tavaria, M., Weaver, B., Reich, N. C., Kola, I., Hertzog, P. J. (1997). Cloning and Characterization of Soluble and Transmembrane Isoforms of a Novel Component of the Murine Type I Interferon Receptor, IFNAR 2. J. Biol. Chem. 272: 23865-23870 [Abstract] [Full Text]  
  • Holland, K. A., Owczarek, C. M., Hwang, S. Y., Tymms, M. J., Constantinescu, S. N., Pfeffer, L. M., Kola, I., Hertzog, P. J. (1997). A Type I Interferon Signaling Factor, ISF21, Encoded on Chromosome 21 Is Distinct from Receptor Components and Their Down-regulation and Is Necessary for Transcriptional Activation of Interferon-regulated Genes. J. Biol. Chem. 272: 21045-21051 [Abstract] [Full Text]  
  • Katzen, A. L., Bishop, J. M. (1996). myb provides an essential function during Drosophila development. Proc. Natl. Acad. Sci. USA 93: 13955-13960 [Abstract] [Full Text]  
  • Owczarek, C. M., Layton, M. J., Robb, L. G., Nicola, N. A., Begley, C. G. (1996). Molecular Basis of the Soluble and Membrane-bound Forms of the Murine Leukemia Inhibitory Factor Receptor alpha-Chain. J. Biol. Chem. 271: 5495-5504 [Abstract] [Full Text]  
  • Dike, L., Ingber, D. (1996). Integrin-dependent induction of early growth response genes in capillary endothelial cells. J. Cell Sci. 109: 2855-2863 [Abstract]  
  • Toth, C. R., Hostutler, R. F., Baldwin, A. S.:Jr., Bender, T. P. (1995). Members of the Nuclear Factor kappaB Family Transactivate the Murine c-myb Gene. J. Biol. Chem. 270: 7661-7671 [Abstract] [Full Text]  
  • Chow, R., Roux, G., Roghani, M, Palmer, M., Rifkin, D., Moscatelli, D., Lang, R. (1995). FGF suppresses apoptosis and induces differentiation of fibre cells in the mouse lens. Development 121: 4383-4393 [Abstract]  
  • Ness, S A, Kowenz-Leutz, E, Casini, T, Graf, T, Leutz, A (1993). Myb and NF-M: combinatorial activators of myeloid genes in heterologous cell types.. Genes Dev. 7: 749-759 [Abstract]  
  • Leonard, M., Lim, K., Engel, J. (1993). Expression of the chicken GATA factor family during early erythroid development and differentiation. Development 119: 519-531 [Abstract]  
  • Murphy, M, Reid, K, Brown, M., Bartlett, P. (1993). Involvement of leukemia inhibitory factor and nerve growth factor in the development of dorsal root ganglion neurons. Development 117: 1173-1182 [Abstract]  
  • Luscher, B, Eisenman, R N (1990). New light on Myc and Myb. Part II. Myb.. Genes Dev. 4: 2235-2241  
  • Tice-Baldwin, K, Fink, G., Arndt, K. (1989). BAS1 has a Myb motif and activates HIS4 transcription only in combination with BAS2. Science 246: 931-935 [Abstract]  
  • Klempnauer, K H, Arnold, H, Biedenkapp, H (1989). Activation of transcription by v-myb: evidence for two different mechanisms.. Genes Dev. 3: 1582-1589 [Abstract]  
  • Bender, T., Thompson, C., Kuehl, W. (1987). Differential expression of c-myb mRNA in murine B lymphomas by a block to transcription elongation. Science 237: 1473-1476 [Abstract]  
  • Barletta, C, Pelicci, P., Kenyon, L., Smith, S., Dalla-Favera, R (1987). Relationship between the c-myb locus and the 6q-chromosomal aberration in leukemias and lymphomas. Science 235: 1064-1067 [Abstract]  
  • Stern, J., Smith, K. (1986). Interleukin-2 induction of T-cell G1 progression and c-myb expression. Science 233: 203-206 [Abstract]  
  • Bishop, J.M., Drees, B., Katzen, A.L., Kornberg, T.B., Simon, M.A. (1985). Proto-oncogenes of Drosophila melanogaster. Cold Spring Harb Symp Quant Biol 50: 727-731 [Abstract]  
  • Mountz, J., Steinberg, A., Klinman, D., Smith, H., Mushinski, J. (1984). Autoimmunity and increased c-myb transcription. Science 226: 1087-1089 [Abstract]  
  • Stewart, T., Bellve, A., Leder, P (1984). Transcription and promoter usage of the myc gene in normal somatic and spermatogenic cells. Science 226: 707-710 [Abstract]  
  • CLINE, M. J., SLAMON, D. J., LIPSICK, J. S. (1984). Oncogenes: Implications for the Diagnosis and Treatment of Cancer. ANN INTERN MED 101: 223-233 [Abstract]  
  • Slamon, D., deKernion, J., Verma, I., Cline, M. (1984). Expression of cellular oncogenes in human malignancies. Science 224: 256-262 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»