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Nuclear Inheritance of Erythromycin Resistance in Human Cells: New Class of Mitochondrial Protein Synthesis Mutants

Claus-Jens Doersen and Eric J. Stanbridge

¹ Department of Microbiology, California College of Medicine, University of California, Irvine, California 92717

ABSTRACT

The characterization of two new erythromycin-resistant mutants of HeLa cells is described. The strains ERY2305 and ERY2309 both exhibited resistance to erythromycin in growth assays and cell-free mitochondrial protein synthesis assays. The erythromycin resistance phenotype could not be transferred by cybridization. The mutation appeared to be encoded in the nucleus and inherited as a recessive trait. These two mutants, therefore, represent a new class of erythromycin-resistant mutants in human cells that is distinct from the cytoplasmically inherited mutation in strain ERY2301 described previously.

Present address: Division of Biology, California Institute of Technology, Pasadena, CA 91125