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Mol Cell Biol. 1982 June; 2(6): 701-707

Further Evidence That the Majority of Primary Nuclear RNA Transcripts in Mammalian Cells Do Not Contribute to mRNA

¹ The Rockefeller University, New York, New York 10021

ABSTRACT

Nuclear RNA from Chinese hamster ovary cells was effectively separated into polyadenylic acid [poly(A)]-containing [poly (A)⁺] and non-poly(A)-containing [poly(A)^–] fractions so that 90% of the poly(A) was present in the (A)⁺ fraction. Only 25% of the 5'-terminal caps of the large nuclear molecules were present in the (A)⁺ class, but about 70% of the specific mRNA sequences (assayed with cDNA clones) were in the (A)⁺ class. It appears that many long capped heterogeneous nuclear RNA molecules are of a different sequence category from those molecules that are successfully processed into mRNA.

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