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Molecular and Cellular Biology, January 2000, p. 12-25, Vol. 20, No. 1
Institute of Molecular and Cell Biology,
National University of Singapore, Singapore 117609, Singapore
Received 8 June 1999/Returned for modification 28 July
1999/Accepted 28 September 1999
The EH domain proteins Pan1p and End3p of budding yeast have been
known to form a complex in vivo and play important roles in
organization of the actin cytoskeleton and endocytosis. In this report,
we describe new findings concerning the function of the Pan1p-End3p
complex. First, we found that the Pan1p-End3p complex associates with
Sla1p, another protein known to be required for the assembly of
cortical actin structures. Sla1p interacts with the first long repeat
region of Pan1p and the N-terminal EH domain of End3p, thus leaving the
Pan1p-End3p interaction, which requires the second long repeat of Pan1p
and the C-terminal repeat region of End3p, undisturbed. Second, Pan1p,
End3p, and Sla1p are also required for normal cell wall morphogenesis.
Each of the Pan1-4, sla1
0270-7306/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Pan1p, End3p, and Sla1p, Three Yeast Proteins Required for Normal
Cortical Actin Cytoskeleton Organization, Associate with Each Other
and Play Essential Roles in Cell Wall Morphogenesis
, and
end3
mutants displays the abnormal cell wall morphology
previously reported for the act1-1 mutant. These cell wall
defects are also exhibited by wild-type cells overproducing the
C-terminal region of Sla1p that is responsible for interactions with
Pan1p and End3p. These results indicate that the functions of Pan1p,
End3p, and Sla1p in cell wall morphogenesis may depend on the formation
of a heterotrimeric complex. Interestingly, the cell wall abnormalities
exhibited by these cells are independent of the actin cytoskeleton
organization on the cell cortex, as they manifest despite the presence
of apparently normal cortical actin cytoskeleton. Examination of
several act1 mutants also supports this conclusion. These
observations suggest that the Pan1p-End3p-Sla1p complex is required not
only for normal actin cytoskeleton organization but also for normal
cell wall morphogenesis in yeast.
*
Corresponding author. Mailing address: Institute of
Molecular and Cell Biology, National University of Singapore, 30 Medical Dr., Singapore 117609, Singapore. Phone: (65)8743382. Fax:
(65)7791117. E-mail: mcbcaimj{at}imcb.nus.edu.sg.
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