This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ochsenbauer, C.
Right arrow Articles by Hunter, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ochsenbauer, C.
Right arrow Articles by Hunter, E.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, January 2000, p. 249-260, Vol. 20, No. 1
0270-7306/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

The Rous Sarcoma Virus Env Glycoprotein Contains a Highly Conserved Motif Homologous to Tyrosine-Based Endocytosis Signals and Displays an Unusual Internalization Phenotype

Christina Ochsenbauer, Susan R. Dubay, and Eric Hunter*

Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294

Received 2 August 1999/Returned for modification 1 September 1999/Accepted 5 October 1999

The cytoplasmic domains of retroviral transmembrane (TM) glycoproteins contain conserved sequence motifs that resemble tyrosine-based (YXXØ-type) endocytosis signals. We have previously described a mutant Rous sarcoma virus (RSV) Env protein, Env-µ26, with an L165R mutation in the membrane-spanning domain (MSD) of TM, that exhibited dramatically decreased steady-state surface expression (G. L. Davis and E. Hunter, J. Cell Biol. 105:1191-1203, 1987; P. B. Johnston, J. Y. Dong, and E. Hunter, Virology 206:353-361, 1995). We now demonstrate that the tyrosine of the Y190RKM motif in the RSV TM cytoplasmic domain is crucial for the µ26 phenotype and is part of an efficient internalization signal in the context of a mutant MSD. In contrast, despite the presence of the Y190RKM motif, wild-type RSV Env is constitutively internalized at a slow rate (1.1%/min) more characteristic of bulk uptake during membrane turnover than of active clustering into endocytic vesicles. The µ26 mutation and two MSD mutations that abrogate palmitoylation of TM resulted in enhanced Env endocytosis indicative of active concentration into coated pits. Surprisingly, an Env-Y190A mutant was apparently excluded from coated pits since its uptake rate of 0.3%/min was significantly below that expected for the bulk rate. We suggest that in RSV Env an inherently functional endocytosis motif is silenced by a counteracting determinant in the MSD that acts to prevent clustering of Env into endocytic vesicles. Mutations in either the cytoplasmic tail or the MSD that inactivate one of the two counteracting signals would thus render the remaining determinant dominant.

* Corresponding author. Mailing address: Department of Microbiology, University of Alabama at Birmingham, 845 19th St. South, Birmingham, AL 35294. Phone: (205) 934-4321. Fax: (205) 934-1640. E-mail: ehunter{at}uab.edu.

Molecular and Cellular Biology, January 2000, p. 249-260, Vol. 20, No. 1
0270-7306/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Song, C., Micoli, K., Bauerova, H., Pichova, I., Hunter, E. (2005). Amino Acid Residues in the Cytoplasmic Domain of the Mason-Pfizer Monkey Virus Glycoprotein Critical for Its Incorporation into Virions. J. Virol. 79: 11559-11568 [Abstract] [Full Text]  
  • Song, C., Micoli, K., Hunter, E. (2005). Activity of the Mason-Pfizer Monkey Virus Fusion Protein Is Modulated by Single Amino Acids in the Cytoplasmic Tail. J. Virol. 79: 11569-11579 [Abstract] [Full Text]  
  • Chan, W.-E., Lin, H.-H., Chen, S. S.-L. (2005). Wild-Type-Like Viral Replication Potential of Human Immunodeficiency Virus Type 1 Envelope Mutants Lacking Palmitoylation Signals. J. Virol. 79: 8374-8387 [Abstract] [Full Text]  
  • Tanzi, G. O., Piefer, A. J., Bates, P. (2003). Equine Infectious Anemia Virus Utilizes Host Vesicular Protein Sorting Machinery during Particle Release. J. Virol. 77: 8440-8447 [Abstract] [Full Text]  
  • Song, C., Dubay, S. R., Hunter, E. (2003). A Tyrosine Motif in the Cytoplasmic Domain of Mason-Pfizer Monkey Virus Is Essential for the Incorporation of Glycoprotein into Virions. J. Virol. 77: 5192-5200 [Abstract] [Full Text]  
  • Aguilar, H. C., Anderson, W. F., Cannon, P. M. (2002). Cytoplasmic Tail of Moloney Murine Leukemia Virus Envelope Protein Influences the Conformation of the Extracellular Domain: Implications for Mechanism of Action of the R Peptide. J. Virol. 77: 1281-1291 [Abstract] [Full Text]  
  • Li, M., Yang, C., Tong, S., Weidmann, A., Compans, R. W. (2002). Palmitoylation of the Murine Leukemia Virus Envelope Protein Is Critical for Lipid Raft Association and Surface Expression. J. Virol. 76: 11845-11852 [Abstract] [Full Text]  
  • Ochsenbauer-Jambor, C., Delos, S. E., Accavitti, M. A., White, J. M., Hunter, E. (2002). Novel Monoclonal Antibody Directed at the Receptor Binding Site on the Avian Sarcoma and Leukosis Virus Env Complex. J. Virol. 76: 7518-7527 [Abstract] [Full Text]  
  • West, J. T., Weldon, S. K., Wyss, S., Lin, X., Yu, Q., Thali, M., Hunter, E. (2002). Mutation of the Dominant Endocytosis Motif in Human Immunodeficiency Virus Type 1 gp41 Can Complement Matrix Mutations without Increasing Env Incorporation. J. Virol. 76: 3338-3349 [Abstract] [Full Text]  
  • Ochsenbauer-Jambor, C., Miller, D. C., Roberts, C. R., Rhee, S. S., Hunter, E. (2001). Palmitoylation of the Rous Sarcoma Virus Transmembrane Glycoprotein Is Required for Protein Stability and Virus Infectivity. J. Virol. 75: 11544-11554 [Abstract] [Full Text]  
  • Zeilfelder, U., Bosch, V. (2001). Properties of Wild-Type, C-Terminally Truncated, and Chimeric Maedi-Visna Virus Glycoprotein and Putative Pseudotyping of Retroviral Vector Particles. J. Virol. 75: 548-555 [Abstract] [Full Text]  
  • Grange, M.-P., Blot, V., Delamarre, L., Bouchaert, I., Rocca, A., Dautry-Varsat, A., Dokhélar, M.-C. (2000). Identification of Two Intracellular Mechanisms Leading to Reduced Expression of Oncoretrovirus Envelope Glycoproteins at the Cell Surface. J. Virol. 74: 11734-11743 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»