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Molecular and Cellular Biology, January 2000, p. 363-371, Vol. 20, No. 1
0270-7306/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

E2F Is Required To Prevent Inappropriate S-Phase Entry of Mammalian Cells

Song He,1 Brian L. Cook,1 Benjamin E. Deverman,1 Ulrich Weihe,1 Fan Zhang,1 Vivek Prachand,2 Jie Zheng,1 and Steven J. Weintraub1,*

Departments of Internal Medicine and of Cell Biology and Physiology1 and Department of Surgery,2 Washington University School of Medicine, St. Louis, Missouri 63110

Received 27 July 1999/Returned for modification 13 September 1999/Accepted 5 October 1999

E2F is a family of transcription factors that regulates the cell cycle. It is widely accepted that E2F-mediated transactivation of a set of genes is the critical activity that governs cellular progression through G1 into S phase. In contrast to this hypothesis, we demonstrate that E2F actually suppresses the onset of S phase in two cell types when the cells are arrested by gamma irradiation. Our findings indicate that in these cells, the critical event triggering progression from G0/G1 arrest into S phase is the release of E2F-mediated transrepression of cell cycle genes, not transactivation by E2F. Furthermore, our data suggest that E2F-mediated transactivation is not necessary for the G1/S-phase transition in these cells.

* Corresponding author. Mailing address: Departments of Internal Medicine and of Cell Biology and Physiology, Washington University School of Medicine, 660 South Euclid Ave., Campus Box 8052, St. Louis, MO 63110. Phone: (314) 362-8964. Fax: (314) 362-8963. E-mail: weintrau{at}im.wustl.edu.

Molecular and Cellular Biology, January 2000, p. 363-371, Vol. 20, No. 1
0270-7306/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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