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Molecular and Cellular Biology, May 2000, p. 3434-3441, Vol. 20, No. 10
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

SINE Retroposons Can Be Used In Vivo as Nucleation Centers for De Novo Methylation

Philippe Arnaud, Chantal Goubely, Thierry Pélissier, and Jean-Marc Deragon*

Biomove, UMR6547 CNRS, Université Blaise Pascal Clermont-Ferrand II, 63177 Aubière Cedex, France

Received 22 September 1999/Returned for modification 31 October 1999/Accepted 28 February 2000

SINEs (short interspersed elements) are an abundant class of transposable elements found in a wide variety of eukaryotes. Using the genomic sequencing technique, we observed that plant S1 SINE retroposons mainly integrate in hypomethylated DNA regions and are targeted by methylases. Methylation can then spread from the SINE into flanking genomic sequences, creating distal epigenetic modifications. This methylation spreading is vectorially directed upstream or downstream of the S1 element, suggesting that it could be facilitated when a potentially good methylatable sequence is single stranded during DNA replication, particularly when located on the lagging strand. Replication of a short methylated DNA region could thus lead to the de novo methylation of upstream or downstream adjacent sequences.

* Corresponding author. Mailing address: Biomove, UMR6547 CNRS, Université Blaise Pascal Clermont-Ferrand II, 24 Ave des Landais, 63177 Aubière Cedex, France. Phone: 33 473407752. Fax: 33 473407777. E-mail: J-Marc.Deragon{at}geem.univ-bpclermont.fr.

Molecular and Cellular Biology, May 2000, p. 3434-3441, Vol. 20, No. 10
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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