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Molecular and Cellular Biology, May 2000, p. 3459-3469, Vol. 20, No. 10
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Fission Yeast Eso1p Is Required for Establishing Sister Chromatid Cohesion during S Phase

Koichi Tanaka,1 Toshihiro Yonekawa,1,dagger Yosuke Kawasaki,2 Mihoko Kai,1,Dagger Kanji Furuya,2 Masaomi Iwasaki,1,dagger Hiroshi Murakami,1,§ Mitsuhiro Yanagida,2 and Hiroto Okayama1,*

Department of Biochemistry and Molecular Biology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033,1 and Department of Gene Mechanisms, Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606-8502,2 Japan

Received 29 November 1999/Returned for modification 10 January 2000/Accepted 28 February 2000

Sister chromatid cohesion is essential for cell viability. We have isolated a novel temperature-sensitive lethal mutant named eso1-H17 that displays spindle assembly checkpoint-dependent mitotic delay and abnormal chromosome segregation. At the permissive temperature, the eso1-H17 mutant shows mild sensitivity to UV irradiation and DNA-damaging chemicals. At the nonpermissive temperature, the mutant is arrested in M phase with a viability loss due to a failure to establish sister chromatid cohesion during S phase. The lethal M-phase arrest phenotype, however, is suppressed by inactivation of a spindle checkpoint. The eso1+ gene is not essential for the onset and progression of DNA replication but has remarkable genetic interactions with those genes regulating the G1-S transition and DNA replication. The N-terminal two-thirds of Eso1p is highly homologous to DNA polymerase eta  of budding yeast and humans, and the C-terminal one-third is homologous to budding yeast Eco1p (also called Ctf7p), which is required for the establishment of sister chromatid cohesion. Deletion analysis and determination of the mutation site reveal that the function of the Eco1p/Ctf7p-homologous domain is necessary and sufficient for sister chromatid cohesion. On the other hand, deletion of the DNA polymerase eta  domain in Eso1p increases sensitivity to UV irradiation. These results indicate that Eso1p plays a dual role during DNA replication. The C-terminal region acts to establish sister chromatid cohesion, and the N-terminal region presumably catalyzes translesion DNA synthesis when template DNA contains lesions that block regular DNA replication.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, The University of Tokyo, Graduate School of Medicine, Bunkyo-ku, Tokyo 113-0033, Japan. Phone: 81-3-5689-0876. Fax: 81-3-3815-1490. E-mail: okayama{at}m.u-tokyo.ac.jp.

dagger Present address: Biochemical Research Laboratory, Eiken Chemical Co. Ltd., Tochigi, Japan.

Dagger Present address: Department of Pathology, School of Medicine, Stanford University, Stanford, CA 94305-5324.

§ Present address: Cell Cycle Laboratory, Imperial Cancer Research Fund, London WC2A 3PX, United Kingdom.

Molecular and Cellular Biology, May 2000, p. 3459-3469, Vol. 20, No. 10
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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