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Molecular and Cellular Biology, May 2000, p. 3558-3567, Vol. 20, No. 10
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Multiple Mechanisms Control Phosphorylation of PHAS-I in Five (S/T)P Sites That Govern Translational Repression

Isabelle Mothe-Satney,1 Daqing Yang,1,dagger Patrick Fadden,1 Timothy A. J. Haystead,1 and John C. Lawrence Jr.1,2,*

Departments of Pharmacology1 and Medicine,2 University of Virginia School of Medicine, Charlottesville, Virginia 22908

Received 16 September 1999/Returned for modification 1 November 1999/Accepted 3 February 2000

Control of the translational repressor, PHAS-I, was investigated by expressing proteins with Ser/Thr right-arrow Ala mutations in the five (S/T)P phosphorylation sites. Results of experiments with HEK293 cells reveal at least three levels of control. At one extreme is nonregulated phosphorylation, exemplified by constitutive phosphorylation of Ser82. At an intermediate level, amino acids and insulin stimulate the phosphorylation of Thr36, Thr45, and Thr69 via mTOR-dependent processes that function independently of other sites in PHAS-I. At the third level, the extent of phosphorylation of one site modulates the phosphorylation of another. This control is represented by Ser64 phosphorylation, which depends on the phosphorylation of all three TP sites. The five sites have different influences on the electrophoretic properties of PHAS-I and on the affinity of PHAS-I for eukaryotic initiation factor 4E (eIF4E). Phosphorylation of Thr45 or Ser64 results in the most dramatic decreases in eIF4E binding in vitro. However, each of the sites influences mRNA translation, either directly by modulating the binding affinity of PHAS-I and eIF4E or indirectly by affecting the phosphorylation of other sites.

* Corresponding author. Mailing address: Department of Pharmacology, University of Virginia Health System, P.O. Box 800735, Charlottesville, VA 22908. Phone: (804) 924-1584. Fax: (804) 982-3575. E-mail: JCL3p{at}Virginia.edu.

dagger Present address: Department of Hematology and Oncology, St. Judes Children's Hospital, 335 North Lauderdale, Memphis, TN 38105.

Molecular and Cellular Biology, May 2000, p. 3558-3567, Vol. 20, No. 10
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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