Repression of Ribosome and tRNA Synthesis in Secretion-Defective Cells Is Signaled by a Novel Branch of the Cell Integrity Pathway

doi:10.1128/MCB.20.11.3843-3851.2000

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Molecular and Cellular Biology, June 2000, p. 3843-3851, Vol. 20, No. 11
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Repression of Ribosome and tRNA Synthesis in Secretion-Defective Cells Is Signaled by a Novel Branch of the Cell Integrity Pathway

Yun Li,¹ Robyn D. Moir,¹ Indra K. Sethy-Coraci,¹^, Jonathan R. Warner,² and Ian M. Willis¹^,^*

Departments of Biochemistry¹ and Cell Biology,² Albert Einstein College of Medicine, Bronx, New York 10461

Received 12 January 2000/Returned for modification 14 February 2000/Accepted 8 March 2000

The transcription of ribosomal DNA, ribosomal protein (RP) genes, and 5S and tRNA genes by RNA polymerases (Pols) I, II, andIII, respectively, is rapidly and coordinately repressed uponinterruption of the secretory pathway in Saccharomyces cerevisiae.We find that repression of ribosome and tRNA synthesis in secretion-defectivecells involves activation of the cell integrity pathway. Transcriptionalrepression requires the upstream components of this pathway, includingthe Wsc family of putative plasma membrane sensors and proteinkinase C (PKC), but not the downstream Bck1-Mkk1/2-Slt2 mitogen-activatedprotein kinase cascade. These findings reveal a novel PKC effectorpathway that controls more than 85% of nuclear transcription.It is proposed that the coordination of ribosome and tRNA synthesiswith cell growth may be achieved, in part, by monitoring the turgorpressure of thecell.

^* Corresponding author. Mailing address: Department of Biochemistry, Albert Einstein College of Medicine, 1300 Morris Park Ave.,Bronx, NY 10461. Phone: (718) 430-2839. Fax: (718) 430-8565. E-mail:willis{at}aecom.yu.edu.

Present address: Department of Physiology and Cellular Biophysics, College of Physicians and Surgeons, Columbia University,New York, NY10032.

Molecular and Cellular Biology, June 2000, p. 3843-3851, Vol. 20, No. 11
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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