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Molecular and Cellular Biology, June 2000, p. 4295-4308, Vol. 20, No. 12
Ruprecht-Karls-Universität Heidelberg,
Biochemie-Zentrum Heidelberg (BZH),1 and
Abteilung Molekulare Biologie der Mitose, Deutsches
Krebsforschungszentrum,3 D-69120 Heidelberg,
Germany, and Centre Hospitalier Universitaire Vaudois,
Microbiologie, CH-1011 Lausanne, Switzerland2
Received 1 November 1999/Returned for modification 8 December
1999/Accepted 29 March 2000
The RanGTP-binding protein RanBP1, which is located in
the cytoplasm, has been implicated in release of nuclear export
complexes from the cytoplasmic side of the nuclear pore complex. Here
we show that Yrb1 (the yeast homolog of RanBP1) shuttles between the
nucleus and the cytoplasm. Nuclear import of Yrb1 is a facilitated process that requires a short basic sequence within the Ran-binding domain (RBD). By contrast, nuclear export of Yrb1 requires an intact
RBD, which forms a ternary complex with the Xpo1 (Crm1) NES receptor in
the presence of RanGTP. Nuclear export of Yrb1, however, is
insensitive towards leptomycin B, suggesting a novel type of substrate
recognition between Yrb1 and Xpo1. Taken together, these data suggest
that ongoing nuclear import and export is an important feature of Yrb1
function in vivo.
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Yeast Ran-Binding Protein 1 (Yrb1) Shuttles between the Nucleus
and Cytoplasm and Is Exported from the Nucleus via a CRM1
(XPO1)-Dependent Pathway
*
Corresponding author. Mailing address:
Biochemie-Zentrum Heidelberg (BZH), Im Neuenheimer Feld 328, 4. OG,
D-69120 Heidelberg, Germany. Phone: 49-6221-544173. Fax:
49-6221-544369. E-mail:
cg5{at}ix.urz.uni-heidelberg.de.
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