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Molecular and Cellular Biology, June 2000, p. 4295-4308, Vol. 20, No. 12
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Yeast Ran-Binding Protein 1 (Yrb1) Shuttles between the Nucleus and Cytoplasm and Is Exported from the Nucleus via a CRM1 (XPO1)-Dependent Pathway

Markus Künzler,1 Thomas Gerstberger,1 Françoise Stutz,2 F. Ralf Bischoff,3 and Ed Hurt1,*

Ruprecht-Karls-Universität Heidelberg, Biochemie-Zentrum Heidelberg (BZH),1 and Abteilung Molekulare Biologie der Mitose, Deutsches Krebsforschungszentrum,3 D-69120 Heidelberg, Germany, and Centre Hospitalier Universitaire Vaudois, Microbiologie, CH-1011 Lausanne, Switzerland2

Received 1 November 1999/Returned for modification 8 December 1999/Accepted 29 March 2000

The RanGTP-binding protein RanBP1, which is located in the cytoplasm, has been implicated in release of nuclear export complexes from the cytoplasmic side of the nuclear pore complex. Here we show that Yrb1 (the yeast homolog of RanBP1) shuttles between the nucleus and the cytoplasm. Nuclear import of Yrb1 is a facilitated process that requires a short basic sequence within the Ran-binding domain (RBD). By contrast, nuclear export of Yrb1 requires an intact RBD, which forms a ternary complex with the Xpo1 (Crm1) NES receptor in the presence of RanGTP. Nuclear export of Yrb1, however, is insensitive towards leptomycin B, suggesting a novel type of substrate recognition between Yrb1 and Xpo1. Taken together, these data suggest that ongoing nuclear import and export is an important feature of Yrb1 function in vivo.

* Corresponding author. Mailing address: Biochemie-Zentrum Heidelberg (BZH), Im Neuenheimer Feld 328, 4. OG, D-69120 Heidelberg, Germany. Phone: 49-6221-544173. Fax: 49-6221-544369. E-mail: cg5{at}ix.urz.uni-heidelberg.de.

Molecular and Cellular Biology, June 2000, p. 4295-4308, Vol. 20, No. 12
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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