This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dorris, D. R.
Right arrow Articles by Struhl, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dorris, D. R.
Right arrow Articles by Struhl, K.

 Previous Article  |  Next Article 

Molecular and Cellular Biology, June 2000, p. 4350-4358, Vol. 20, No. 12
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Artificial Recruitment of TFIID, but Not RNA Polymerase II Holoenzyme, Activates Transcription in Mammalian Cells

David R. Dorris and Kevin Struhl*

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115

Received 3 February 2000/Returned for modification 16 March 2000/Accepted 23 March 2000

In yeast cells, transcriptional activation occurs when the RNA polymerase II (Pol II) machinery is artificially recruited to a promoter by fusing individual components of this machinery to a DNA-binding domain. Here, we show that artificial recruitment of components of the TFIID complex can activate transcription in mammalian cells. Surprisingly, artificial recruitment of TATA-binding protein (TBP) activates transiently transfected and chromosomally integrated promoters with equal efficiency, whereas artificial recruitment of TBP-associated factors activates only chromosomal reporters. In contrast, artificial recruitment of various components of the mammalian Pol II holoenzyme does not confer transcriptional activation, nor does it result in synergistic activation in combination with natural activation domains. In the one case examined in more detail, the Srb7 fusion failed to activate despite being associated with the Pol II holoenzyme and being directly recruited to the promoter. Interestingly, some acidic activation domains are less effective when the promoter is chromosomally integrated rather than transiently transfected, whereas the Sp1 glutamine-rich activation domain is more effective on integrated reporters. Thus, yeast and mammalian cells differ with respect to transcriptional activation by artificial recruitment of the Pol II holoenzyme.

* Corresponding author. Mailing address: Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115. Phone: (617) 432-2104. Fax: (617) 432-2529. E-mail: kevin{at}hms.harvard.edu.

Molecular and Cellular Biology, June 2000, p. 4350-4358, Vol. 20, No. 12
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Young, E. T., Tachibana, C., Chang, H.-W. E., Dombek, K. M., Arms, E. M., Biddick, R. (2008). Artificial Recruitment of Mediator by the DNA-Binding Domain of Adr1 Overcomes Glucose Repression of ADH2 Expression. Mol. Cell. Biol. 28: 2509-2516 [Abstract] [Full Text]  
  • Copik, A. J., Webb, M. S., Miller, A. L., Wang, Y., Kumar, R., Thompson, E. B. (2006). Activation Function 1 of Glucocorticoid Receptor Binds TATA-Binding Protein in Vitro and in Vivo. Mol. Endocrinol. 20: 1218-1230 [Abstract] [Full Text]  
  • Das, S., Ward, S. V., Tacke, R. S., Suske, G., Samuel, C. E. (2006). Activation of the RNA-dependent Protein Kinase PKR Promoter in the Absence of Interferon Is Dependent Upon Sp Proteins. J. Biol. Chem. 281: 3244-3253 [Abstract] [Full Text]  
  • Hori, R. T., Xu, S., Hu, X., Pyo, S. (2004). TFIIB-facilitated recruitment of preinitiation complexes by a TAF-independent mechanism. Nucleic Acids Res 32: 3856-3863 [Abstract] [Full Text]  
  • Jacobson, S., Pillus, L. (2004). Molecular Requirements for Gene Expression Mediated by Targeted Histone Acetyltransferases. Mol. Cell. Biol. 24: 6029-6039 [Abstract] [Full Text]  
  • Wu, S.-Y., Zhou, T., Chiang, C.-M. (2003). Human Mediator Enhances Activator-Facilitated Recruitment of RNA Polymerase II and Promoter Recognition by TATA-Binding Protein (TBP) Independently of TBP-Associated Factors. Mol. Cell. Biol. 23: 6229-6242 [Abstract] [Full Text]  
  • Fujita, H., Fujii, R., Aratani, S., Amano, T., Fukamizu, A., Nakajima, T. (2003). Antithetic Effects of MBD2a on Gene Regulation. Mol. Cell. Biol. 23: 2645-2657 [Abstract] [Full Text]  
  • Louie, M. C., Yang, H. Q., Ma, A.-H., Xu, W., Zou, J. X., Kung, H.-J., Chen, H.-W. (2003). Androgen-induced recruitment of RNA polymerase II to a nuclear receptor-p160 coactivator complex. Proc. Natl. Acad. Sci. USA 100: 2226-2230 [Abstract] [Full Text]  
  • Park, J. M., Kim, J. M., Kim, L. K., Kim, S. N., Kim-Ha, J., Kim, J. H., Kim, Y.-J. (2003). Signal-Induced Transcriptional Activation by Dif Requires the dTRAP80 Mediator Module. Mol. Cell. Biol. 23: 1358-1367 [Abstract] [Full Text]  
  • Kobayashi, A., Miyake, T., Kawaichi, M., Kokubo, T. (2003). Mutations in the histone fold domain of the TAF12 gene show synthetic lethality with the TAF1 gene lacking the TAF N-terminal domain (TAND) by different mechanisms from those in the SPT15 gene encoding the TATA box-binding protein (TBP). Nucleic Acids Res 31: 1261-1274 [Abstract] [Full Text]  
  • Luciano, R. L., Wilson, A. C. (2002). An activation domain in the C-terminal subunit of HCF-1 is important for transactivation by VP16 and LZIP. Proc. Natl. Acad. Sci. USA 99: 13403-13408 [Abstract] [Full Text]  
  • Deckert, J., Struhl, K. (2002). Targeted Recruitment of Rpd3 Histone Deacetylase Represses Transcription by Inhibiting Recruitment of Swi/Snf, SAGA, and TATA Binding Protein. Mol. Cell. Biol. 22: 6458-6470 [Abstract] [Full Text]  
  • Vassallo, M. F., Tanese, N. (2002). Isoform-specific interaction of HP1 with human TAFII130. Proc. Natl. Acad. Sci. USA 99: 5919-5924 [Abstract] [Full Text]  
  • Markus, S. M., Taneja, S. S., Logan, S. K., Li, W., Ha, S., Hittelman, A. B., Rogatsky, I., Garabedian, M. J. (2002). Identification and Characterization of ART-27, a Novel Coactivator for the Androgen Receptor N Terminus. Mol. Biol. Cell 13: 670-682 [Abstract] [Full Text]  
  • Felinski, E. A., Quinn, P. G. (2001). The coactivator dTAFII110/hTAFII135 is sufficient to recruit a polymerase complex and activate basal transcription mediated by CREB. Proc. Natl. Acad. Sci. USA 10.1073/pnas.241337698v1 [Abstract] [Full Text]  
  • Licciardo, P., Ruggiero, L., Lania, L., Majello, B. (2001). Transcription activation by targeted recruitment of the RNA polymerase II CTD phosphatase FCP1. Nucleic Acids Res 29: 3539-3545 [Abstract] [Full Text]  
  • Aratani, S., Fujii, R., Oishi, T., Fujita, H., Amano, T., Ohshima, T., Hagiwara, M., Fukamizu, A., Nakajima, T. (2001). Dual Roles of RNA Helicase A in CREB-Dependent Transcription. Mol. Cell. Biol. 21: 4460-4469 [Abstract] [Full Text]  
  • Felinski, E. A., Kim, J., Lu, J., Quinn, P. G. (2001). Recruitment of an RNA Polymerase II Complex Is Mediated by the Constitutive Activation Domain in CREB, Independently of CREB Phosphorylation. Mol. Cell. Biol. 21: 1001-1010 [Abstract] [Full Text]  
  • Kobayashi, A., Miyake, T., Ohyama, Y., Kawaichi, M., Kokubo, T. (2001). Mutations in the TATA-binding Protein, Affecting Transcriptional Activation, Show Synthetic Lethality with the TAF145 Gene Lacking the TAF N-terminal Domain in Saccharomyces cerevisiae. J. Biol. Chem. 276: 395-405 [Abstract] [Full Text]  
  • Wu, S.-Y., Chiang, C.-M. (2001). TATA-binding Protein-associated Factors Enhance the Recruitment of RNA Polymerase II by Transcriptional Activators. J. Biol. Chem. 276: 34235-34243 [Abstract] [Full Text]  
  • Vassallo, M. F., Tanese, N. (2002). Isoform-specific interaction of HP1 with human TAFII130. Proc. Natl. Acad. Sci. USA 99: 5919-5924 [Abstract] [Full Text]  
  • Felinski, E. A., Quinn, P. G. (2001). The coactivator dTAFII110/hTAFII135 is sufficient to recruit a polymerase complex and activate basal transcription mediated by CREB. Proc. Natl. Acad. Sci. USA 98: 13078-13083 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»