Functional Characterization of the X-Linked Inhibitor of Apoptosis (XIAP) Internal Ribosome Entry Site Element: Role of La Autoantigen in XIAP Translation

doi:10.1128/MCB.20.13.4648-4657.2000

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Molecular and Cellular Biology, July 2000, p. 4648-4657, Vol. 20, No. 13
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Functional Characterization of the X-Linked Inhibitor of Apoptosis (XIAP) Internal Ribosome Entry Site Element: Role of La Autoantigen in XIAP Translation

Martin Holcik and Robert G. Korneluk^*

Apoptogen Inc.; Solange Gauthier Karsh Molecular Genetics Laboratory, Children's Hospital of Eastern Ontario; and Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, Ontario K1H 8L1, Canada

Received 9 February 2000/Returned for modification 22 March 2000/Accepted 11 April 2000

X-linked inhibitor of apoptosis protein (XIAP) is a key regulator of programmed cell death triggered by various apoptotictriggers. Translation of XIAP is controlled by a 162-nucleotide(nt) internal ribosome entry site (IRES) element located in the5' untranslated region of XIAP mRNA. XIAP IRES mediates efficienttranslation of XIAP under physiological stress and enhances cellprotection against serum deprivation and radiation-induced apoptosis.In the present report we describe the assembly of a sequence-specificRNA-protein complex consisting of at least four cytosolic proteinson the XIAP IRES element. We determine that the core binding sequenceis approximately 28 nt long and is located 34 nt upstream of theinitiation site. Moreover, we identify the La autoantigen as aprotein that specifically binds XIAP IRES in vivo and in vitro.The biological relevance of this interaction is further demonstratedby the inhibition of XIAP IRES-mediated translation in the absenceof functional La protein. The results suggest an important rolefor the La protein in the regulation of XIAP expression, possiblyby facilitating ribosome recruitment to the XIAPIRES.

^* Corresponding author. Mailing address: Solange Gauthier Karsh Molecular Genetics Laboratory, Children's Hospital of EasternOntario Research Institute, 401 Smyth Road, Room R306, Ottawa,Ontario K1H 8L1, Canada. Phone: (613) 738-3821. Fax: (613) 738-4833.E-mail: bob{at}mgcheo.med.uottawa.ca.

Molecular and Cellular Biology, July 2000, p. 4648-4657, Vol. 20, No. 13
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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