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Molecular and Cellular Biology, July 2000, p. 4838-4848, Vol. 20, No. 13
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Bypass of a Meiotic Checkpoint by Overproduction of Meiotic Chromosomal Proteins

Julie M. Bailis,1 Albert V. Smith,1,2,dagger and G. Shirleen Roeder1,2,3,*

Howard Hughes Medical Institute,2 Department of Molecular, Cellular, and Developmental Biology,1 and Department of Genetics,3 Yale University, New Haven, Connecticut 06520-8103

Received 17 December 1999/Returned for modification 4 February 2000/Accepted 6 April 2000

The Saccharomyces cerevisiae zip1 mutant, which exhibits defects in synaptonemal complex formation and meiotic recombination, triggers a checkpoint that causes cells to arrest at the pachytene stage of meiotic prophase. Overproduction of either the meiotic chromosomal protein Red1 or the meiotic kinase Mek1 bypasses this checkpoint, allowing zip1 cells to sporulate. Red1 or Mek1 overproduction also promotes sporulation of other mutants (zip2, dmc1, hop2) that undergo checkpoint-mediated arrest at pachytene. In addition, Red1 overproduction antagonizes interhomolog interactions in the zip1 mutant, substantially decreasing double-strand break formation, meiotic recombination, and homologous chromosome pairing. Mek1 overproduction, in contrast, suppresses checkpoint-induced arrest without significantly decreasing meiotic recombination. Cooverproduction of Red1 and Mek1 fails to bypass the checkpoint; moreover, overproduction of the meiotic chromosomal protein Hop1 blocks the Red1 and Mek1 overproduction phenotypes. These results suggest that meiotic chromosomal proteins function in the signaling of meiotic prophase defects and that the correct stoichiometry of Red1, Mek1, and Hop1 is needed to achieve checkpoint-mediated cell cycle arrest at pachytene.

* Corresponding author. Mailing address: Howard Hughes Medical Institute, Department of Molecular, Cellular, and Developmental Biology, Yale University, P.O. Box 208103, New Haven, CT 06520-8103. Phone: (203) 432-3501. Fax: (203) 432-3263. E-mail: shirleen.roeder{at}yale.edu.

dagger Present address: deCODE Genetics, Inc., 110 Reykjavik, Iceland.

Molecular and Cellular Biology, July 2000, p. 4838-4848, Vol. 20, No. 13
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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