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Molecular and Cellular Biology, July 2000, p. 4859-4869, Vol. 20, No. 13
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Arginine N-Methyltransferase 1 Is Required for Early Postimplantation Mouse Development, but Cells Deficient in the Enzyme Are Viable

Maciej R. Pawlak, Christina A. Scherer,dagger Jin Chen,Dagger Michael J. Roshon,§ and H. Earl Ruley*

Department of Microbiology and Immunology, Nashville, Tennessee

Received 10 February 2000/Accepted 5 April 2000

Protein arginine N-methyltransferases have been implicated in a variety of processes, including cell proliferation, signal transduction, and protein trafficking. In this study, we have characterized essentially a null mutation induced by insertion of the U3beta Geo gene trap retrovirus into the second intron of the mouse protein arginine N-methyltransferase 1 gene (Prmt1). cDNAs encoding two forms of Prmt1 were characterized, and the predicted protein sequences were found to be highly conserved among vertebrates. Expression of the Prmt1-beta geo fusion gene was greatest along the midline of the neural plate and in the forming head fold from embryonic day 7.5 (E7.5) to E8.5 and in the developing central nervous system from E8.5 to E13.5. Homozygous mutant embryos failed to develop beyond E6.5, a phenotype consistent with a fundamental role in cellular metabolism. However, Prmt1 was not required for cell viability, as the protein was not detected in embryonic stem (ES) cell lines established from mutant blastocysts. Low levels of Prmt1 transcripts (approximately 1% of the wild-type level) were detected as assessed by a quantitative reverse transcription-PCR assay. Total levels of arginine N-methyltransferase activity and asymmetric NG,NG-dimethylarginine were reduced by 85 and 54%, respectively, while levels of hypomethylated substrates were increased 15-fold. Prmt1 appears to be a major type I enzyme in ES cells, and in wild-type cells, most substrates of the enzyme appear to be maintained in a fully methylated state.

* Corresponding author. Present address: Department of Microbiology and Immunology, Room AA4210 MCN, Vanderbilt University School of Medicine, 1161 21st Ave. South, Nashville, TN 37232-2363. Phone: (615) 343-1379. Fax: (615) 343-7392. E-mail: ruleye{at}ctrvax.vanderbilt.edu.

dagger Present address: Department of Microbiology, University of Washington, Seattle, WA 98195.

Dagger Present address: Department of Medicine, Division of Rheumatology, Vanderbilt University School of Medicine, Nashville, TN 37232-2363.

§ Present address: Department of Emergency Medicine, Carolinas Medical Center, Charlotte, NC 28232-2861.

Molecular and Cellular Biology, July 2000, p. 4859-4869, Vol. 20, No. 13
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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