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Molecular and Cellular Biology, July 2000, p. 5269-5275, Vol. 20, No. 14
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

TATA Binding Protein Can Stimulate Core-Directed Transcription by Yeast RNA Polymerase I

Pavel Aprikian, Beth Moorefield, and Ronald H. Reeder*

Hutchinson Cancer Research Center, Seattle, Washington 98109

Received 10 December 1999/Returned for modification 21 January 2000/Accepted 25 April 2000

The TATA binding protein (TBP) interacts with two transcription factor complexes, upstream activating factor (UAF) and core factor (CF), to direct transcription by RNA polymerase I (polI) in the yeast Saccharomyces cerevisiae. Previous work indicates that one function of TBP is to serve as a bridge, ennabling UAF to recruit and stabilize the binding of CF (23, 24). In this work we show that, in addition to aiding recruitment, TBP also directly aids CF function. Overexpression of TBP in strains with UAF components deleted will stimulate CF-directed transcription nearly to wild-type levels in vivo. In vitro, increasing the concentration of TBP stimulates CF-directed transcription in the absence of either UAF or its DNA binding site. This dual function of TBP, serving as a critical member of a core promoter complex as well as a contact point for upstream activators, appears similar to the dual roles that TBP also plays in transcription by RNA polII.

* Corresponding author. Mailing address: Hutchinson Cancer Research Center, 1100 Fairview Ave. North, Seattle, WA 98109. Phone: (206) 667-4513. Fax: (206) 667-4082. E-mail: rreeder{at}fhcrc.org.

Molecular and Cellular Biology, July 2000, p. 5269-5275, Vol. 20, No. 14
0270-7306/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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